NBL1 (NM_005380) Human 3' UTR Clone

CAT#: SC213934

3`UTR clone of neuroblastoma suppression of tumorigenicity 1 (NBL1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NBL1
• Synonyms: D1S1733E; DAN; DAND1; NB; NO3
• ACCN: NM_005380
• Insert Size: 1333 bp
• Sequence Data:
  Insert Sequence

  >SC213934 3'UTR clone of NM_005380
  The sequence shown below is from the reference sequence of NM_005380. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCCACACAGAGGAAGAGGGGGCTGAGGACTGAGGCCCCCCCAACTCTTCCTCCCCTCTCATCCCCCTGTG
  GAATGTTGGGTCTCACTCTCTGGGGAAGTCAGGGGAGAAGCTGAAGCCCCCCTTTGGCACTGGATGGACT
  TGGCTTCAGACTCGGACTTGAATGCTGCCCGGTTGCCATGGAGATCTGAAGGGGCGGGGTTAGAGCCAAG
  CTGCACAATTTAATATATTCAAGAGTGGGGGGAGGAAGCAGAGGTCTTCAGGGCTCTTTTTTTGGGGGGG
  GTGGTCTCTTCCTGTCTGGCTTCTAGAGATGTGCCTGTGGGAGGGGGAGGAAGTTGGCTGAGCCATTGAG
  TGCTGGGGGAGGCCATCCAAGATGGCATGAATCGGGCTAAGGTCCCTGGGGGTGCAGATGGTACTGCTGA
  GGTCCCGGGCTTAGTGTGAGCATCTTGCCAGCCTCAGGCTTGAGGGAGGGCTGGGCTAGAAAGACCACTG
  GCAGAAACAGGAGGCTCCGGCCCACAGGTTTCCCCAAGGCCTCTCACCCCACTTCCCATCTCCAGGGAAG
  CGTCGCCCCAGTGGCACTGAAGTGGCCCTCCCTCAGCGGAGGGGTTTGGGAGTCAGGCCTGGGCAGGACC
  CTGCTGACTCGTGGCGCGGGAGCTGGGAGCCAGGCTCTCCGGGCCTTTCTCTGGCTTCCTTGGCTTGCCT
  GGTGGGGGAAGGGGAGGAGGGGAAGAAGGAAAGGGAAGAGTCTTCCAAGGCCAGAAGGAGGGGGACAACC
  CCCCAAGACCATCCCTGAAGACGAGCATCCCCCTCCTCTCCCTGTTAGAAATGTTAGTGCCCCGCACTGT
  GCCCCAAGTTCTAGGCCCCCCAGAAAGCTGCCAGAGCCGGCCGCCTTCTCCCCTCTCCCAGGGATGCTCT
  TTGTAAATATCGGATGGGTGTGGGAGTGAGGGGTTACCTCCCTCGCCCCAAGGTTCCAGAGGCCCTAGGC
  GGGATGGGCTCGCTGAACCTCGAGGAACTCCAGGACGAGGAGGACATGGGACTTGCGTGGACAGTCAGGG
  TTCACTTGGGCTCTCTCTAGCTCCCCAATTCTGCCTGCCTCCTCCCTCCCAGCTGCACTTTAACCCTAGA
  AGGTGGGGACCTGGGGGGAGGGACAGGGCAGGCGGGCCCATGAAGAAAGCCCCTCGTTGCCCAGCACTGT
  CTGCGTCTGCTCTTCTGTGCCCAGGGTGGCTGCCAGCCCACTGCCTCCTGCCTGGGGTGGCCTGGCCCTC
  CTGGCTGTTGCGACGCGGGCTTCTGGAGCTTGTCACCATTGGACAGTCTCCCTGATGGACCCTCAGTCTT
  CTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005380.4
• Summary: 'This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013]'
• Locus ID: 4681