CD31 (PECAM1) (NM_000442) Human 3' UTR Clone

CAT#: SC213947

3`UTR clone of platelet/endothelial cell adhesion molecule (PECAM1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PECAM1
• Synonyms: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1
• ACCN: NM_000442
• Insert Size: 1314 bp
• Sequence Data:
  Insert Sequence

  >SC213947 3'UTR clone of NM_000442
  The sequence shown below is from the reference sequence of NM_000442. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGAACGGAAGGCTCCCTTGATGGAACTTAGACAGCAAGGCCAGATGCACATCCCTGGAAGGACATCCATG
  TTCCGAGAAGAACAGATAATCCCTGTATTTCAAGACCTCTGTGCACTTATTTATGAACCTGCCCTGCTCC
  CACAGAACACAGCAATTCCTCAGGCTAAGCTGCCGGTTCTTAAATCCATCCTGCTAAGTTAATGTTGGGT
  AGAAAGAGATACAGAGGGGCTGTTGAATTTCCCACATACCCTCCTTCCACCAAGTTGGAACATCCTTGGA
  AATTGGAAGAGCACAAGAGGAGATCCAGGGCAAGGCCATTGGGATATTCTGAAACTTGAATATTTTGTTT
  TGTGCAGAGATAAAGACCTTTTCCATGCACCCTCATACACAGAAACCAATTTTCTTTTTTATACTCAATC
  ATTTCTAGCGCATGGCCTGGTTAGAGGCTGGTTTTTTCTCTTTTCCTTTGGTCCTTCAAAGGCTTGTAGT
  TTTGGCTAGTCCTTGTTCTTTGGAAATACACAGTGCTGACCAGACAGCCTCCCCCTGTCCCCTCTATGAC
  CTCGCCCTCCACAAATGGGAAAACCAGACTACTTGGGAGCACCGCCTGTGAAATACCAACCTGAAGACAC
  CGTTCATTCAGGCAACGCACAAAACAGAAAATGAAGGTGGAACAAGCACAGATGTTCTTCAACTGTTTTT
  GTCTACACTCTTTCTCTTTTCCTCTACCATGCTGAAGGCTGAAAGACAGGAAGATGGTGCCATCAGCAAA
  TATTATTCTTAATTGAAAACTTGAAATGTGTATGTTTCTTACTAATTTTTAAAAATGTATTCCTTGCCAG
  GGCAGGCAAGGTGGCTCACGCCTGTAATCCCAGCACTTCAGGAGGCTGAGGTGGGCGGATCACCTGAGGT
  CAGGAGTTTGAGACCAGCCTGATGAAACCCTGTCTCTACTAAAAATACAAGAATTAGCCGGGCGTGGTGG
  CGCATGCCTGTAGTATCAGCTACTCAAGAGGCTGAGGTGAGATTATCGCTTGAACCCAGGAAACGGAGGT
  TGTAGTGAGCGGAGATCGCGCCACTGCACTCCAGCCTGAGTGACAGAGTGAGAATCCATCTCAAAAAAAA
  CAAAAAACAAAATTGCTTGCTAAAGAAGTGGTCTCCTGAGGTCTTAAGACATTCCTGACAGTGTCTTGAG
  TGGGTGGGAGAGAGGCTGCTGTCATTGCGCTGTGGAATTTCACAGATGAGAACCACGCCTAGCCAAAATC
  ACTTTTCCTGTTTGCCTCAGTGACACAGCTGCAGGGACCCTCGTGGATGTTGTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000442.3
• Summary: 'The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]'
• Locus ID: 5175