USP33 (NM_015017) Human 3' UTR Clone

CAT#: SC214080

3`UTR clone of ubiquitin specific peptidase 33 (USP33) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: USP33
• Synonyms: VDU1
• ACCN: NM_015017
• Insert Size: 1325
• Sequence Data:
  Insert Sequence

  >SC214080 3'UTR clone of NM_015017
  The sequence shown below is from the reference sequence of NM_015017. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTAGAAACTCGGTCTTTGTAATTTTTAGGATGTAGAGAGTTCTAATGAGGAATCATTTTCATGTGCCCTG
  ACATGTACACATGCGAAAACATTCCTAAAAGCGTGTTTATTTGCTTTATTTTTTTTCATCATTTATCCCA
  TTTATTTCTTCTTAGTGGGCATTATGGAAGAATATATTAAAATGTGTAATATACCACAGGTTGGTATATT
  TAGTTTTAAATACTTACCATAAAGTCTTTCAGTGTAATTTTTTTTTGAGACAGAGTCTTGCTTTGTCACC
  CAGGCTGGAGTGCTGTGGTGTTACCTCAGCTCACTGCAGCCTCCACCTCCTGGGTTCAAGCGATTCTCCT
  GCCTCAGCCTCTCGAGTAGCTGGGATTACAGGCACCTGCCACCATGCCCGGCTAATTTTTGTATTTTAGT
  AGAGATGGGGTTTCACCATGTTGGCCAGGCTAGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCG
  GCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACAGCGCCTGGCCCAGTGTAATATTTTTGAAAGAGG
  AGGGACAATTGTGAAATCAGTAGGTTATCTTTAATCTTTACACTACATGCAGATCCATAGTATCCTTTGT
  AGTGTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTAAATCACCCTGACTCTGACCAGTCTTT
  CAGTTCTCCAAAAGCCCAATTTAATTGTATAGTTTTGTCATGGCTTCATATAATAAAGAGCCTATTTTAA
  GTTGAAAGTAGTAGTCAGAAAATTGTTAATTTCCTAAAGCTCAGGAAACTAGGGTGTCACTTTTTTTGCA
  CTGCAGCATATACACTAACTAGCTTATTAAAATTTACAAAATGTCTTTTTGAATGTATCAAGGATATATT
  TAGTTTGAGTGGAATTTGTCAGCAGATATCAGTAACTTATTGCCGCTTATATTGTACAATGTTAAACTTC
  AATTCCTGTAACCTGGTTAGTATTAATGTCAGTGACTAAAAAACTTAGAGTTAGTTTTAGGGCACTTTTT
  ATTTTGAGAGCATGAAGTGTGGAATGTGTCACTACGATTGTTGATAAAGCTGAGGCCACTTGCAACTTGA
  TTTTTTAAATGAAATAGATAAAGTCTTTTTGAATAATATAGTATGCACTGCTATTTGCTTGATTATGTAA
  TGTCAAAAGTTTAACTATATTCCAAGTACAAAAACATACTGGATTACATTGAGGATGTTGAATAGCATTC
  ATGATGGCTTTGTTTTGGTTTGGGGCAGCTGTCACCAGCTAAAGCAATGTTGTTAAAATTAGCTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_015017.3
• Summary: This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]
• Locus ID: 23032