Endothelin 3 (EDN3) (NM_000114) Human 3' UTR Clone

CAT#: SC214102

3`UTR clone of endothelin 3 (EDN3) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: EDN3
• Synonyms: ET-3; ET3; HSCR4; PPET3; WS4B
• ACCN: NM_000114
• Insert Size: 1313 bp
• Sequence Data:
  Insert Sequence

  >SC214102 3'UTR clone of NM_000114
  The sequence shown below is from the reference sequence of NM_000114. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGCCTCTTTCAGGAAGGAGCCCCTTAGGAGGACAGGCCTGCACTCCGGTTTCATGCAGGAAATTGGTTT
  TGGAGAGTTTTGGCAAGTTGGAAAGCCACTTACTGGCTTTTGACATGACTTCTCTTGGAGAATAAGTGGA
  CTCCAAGCTAACTCTTTGCAAATGTAAACACATGTCCATCTTGTAATAAATGCAAAATGCCCGTGCAGCA
  GAAGCATGCGACTTTCATATCCTTGCCTAGAATAGGCTGCATGGTGTATGTCAGTGAGGGCCACGAGGCG
  TCGGCTTTAGACACAGATCATAGCTCTACAGGAGTTTATGAATTTGAAGCTTATGGGATTTTGGCAGAGA
  AATTTTCAGCTGTGCTTGATACCCACCAAAAGAATGTATCTCGAAAGAATGAAGGAAGAAGAAAAAAGGA
  TCCTTGATGTTTGTGACAAGAAAATGAGAAAGTTAGTATCTGCAATACAGAGCTTGTTCCTGTTCAGTGA
  CTGACCCTCTGTATTCTGTATAGACACCAGGCCGATACACAGTGGAGTTCCCAGGCCTTGTTTGCAGGAA
  GCCGACTGTAAAGACAGCCCCAGCTCAAGGCTATTAGGTTGAATATTTGCTTTCATGAGTAAATGTGGAT
  CTTTGGGGAATGGCTTCAAAATAAGTCACGAACACAAATTCTTTGTAAATTATGTAAATTCCTGTTTATA
  TAAATTGGCAACAACTTATACCGTCTGACAGTTCAAAATCTCTTTCAGCTGCGCTCTTCCCACCGAGCCG
  AGCTTACTGTGAGTGTGGAGATGTTATCCCACCATGTAAAGTCGCCTGCGCAGGGGAGGGCTGCCCATCT
  CCCCAACCCAGTCACAGAGAGATAGGAAACGGCATTTGAGTGGGTGTCCAGGGCCCCGTAGAGAGACATT
  TAAGATGGTGTATGACAGAGCATTGGCCTTGACCAAATGTTAAATCCTCTGTGTGTATTTCATAAGTTAT
  TACAGGTATAAAAGTGATGACCTATCATGAGGAAATGAAAGTGGCTGATTTGCTGGTAGGATTTTGTACA
  GTTTAGAGAAGCGATTATTTATTGTGAAACTGTTCTCCACTCCAACTCCTTTATGTGGATCTGTTCAAAG
  TAGTCACTGTATATACGTATAGAGAGGTAGATAGGTAGGTAGATTTTAAATTGCATTCTGAATACAAACT
  CATACTCCTTAGAGCTTGAATTACATTTTTAAAATGCATATGTGCTGTTTGGCACCGTGGCAAGATGGTA
  TCAGAGAGAAACCCATCAATTGCTCAAATACTCAGAAAGTACTGTCAAAAGCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000114.2
• Summary: 'The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]'
• Locus ID: 1908