HDGF (NM_004494) Human 3' UTR Clone

CAT#: SC214105

3`UTR clone of hepatoma-derived growth factor (high-mobility group protein 1-like) (HDGF) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HDGF
• Synonyms: HMG1L2
• ACCN: NM_004494
• Insert Size: 1344 bp
• Sequence Data:
  Insert Sequence

  >SC214105 3'UTR clone of NM_004494
  The sequence shown below is from the reference sequence of NM_004494. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCAGGCATCAGAGATCATGAGAGCCTGTAGCCACCAATGTTTCAAGAGGAGCCCCCACCCTGTTCCTGCT
  GCTGTCTGGGTGCTACTGGGGAAACTGGCCATGGCCTGCAAACTGGGAACCCCTTTCCCACCCCAACCTG
  CTCTCCTCTTCTACTCACTTTTCCCACTCCAAGCCCAGCCCATGGAGATTGACCTGGATGGGGCAGGCCA
  CCTGGCTCTCACCTCTAGGTCCCCATACTCCTATGATCTGAGTCAGAGCCATGTCTTCTCCCTGGAATGA
  GTTGAGGCCACTGTGTTCCTTCCGCTTGGAGCTATTTTCCAGGCTTCTGCTGGGGCCTGGGACAACTGCT
  CCCACCTCCTGACACCCTTCTCCCACTCTCCTAGGCATTCTGGACCTCTGGGTTGGGATCAGGGGTAGGA
  ATGGAAAGGATGGAGCATCAACAGCAGGGTGGGCTTGTGGGGCCTGGGAGGGGCAATCCTCAAATGCGGG
  GTGGGGGCAGCACAGGAGGGCGGCCTCCTTCTGAGCTCCTGTCCCCTGCTACACCTATTATCCCAGCTGC
  CTAGATTCAGGGAAAGTGGGACAGCTTGTAGGGGAGGGGCTCCTTTCCATAAATCCTTGATGATTGACAA
  CACCCATTTTTCCTTTTGCCGACCCCAAGAGTTTTGGGAGTTGTAGTTAATCATCAAGAGAATTTGGGGC
  TTCCAAGTTGTTCGGGCCAAGGACCTGAGACCTGAAGGGTTGACTTTACCCATTTGGGTGGGAGTGTTGA
  GCATCTGTCCCCCTTTAGATCTCTGAAGCCACAAATAGGATGCTTGGGAAGACTCCTAGCTGTCCTTTTT
  CCTCTCCACACAGTGCTCAAGGCCAGCTTATAGTCATATATATCACCCAGACATAAAGGAAAAGACACAT
  TTTTTAGGAAATGTTTTTAATAAAAGAAAATTACAAAAAAAAATTTTAAAGACCCCTAACCCTTTGTGTG
  CTCTCCATTCTGCTCCTTCCCCATCGTTGCCCCCATTTCTGAGGTGCACTGGGAGGCTCCCCTTCTATTT
  GGGGCTTGATGACTTTCTTTTTGTAGCTGGGGCTTTGATGTTCCTTCCAGTGTCATTTCTCATCCACATA
  CCCTGACCTGGCCCCCTCAGTGTTGTCACCAGATCTGATTTGTAACCCACTGAGAGGACAGAGAGAAATA
  AGTGCCCTCTCCCACCCTCTTCCTACTGGTCTCTCTATGCCTCTCTACAGTCTCGTCTCTTTTACCCTGG
  CCCCTCTCCCTTGGGCTCTGATGAAAAATTGCTGACTGTAGCTTTGGAAGTTTAGCTCTGAGAACCGTAG
  ATGATTTCAGTTCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004494.2
• Summary: 'This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016]'
• Locus ID: 3068