JNK2 (MAPK9) (NM_001135044) Human 3' UTR Clone

CAT#: SC214189

3`UTR clone of mitogen-activated protein kinase 9 (MAPK9) transcript variant JNK2-g for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MAPK9
• Synonyms: JNK-55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; p54a; p54aSAPK; PRKM9; SAPK; SAPK1a
• ACCN: NM_001135044
• Insert Size: 1388 bp
• Sequence Data:
  Insert Sequence

  >SC214189 3'UTR clone of NM_001135044
  The sequence shown below is from the reference sequence of NM_001135044. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGACCGTATCCTTCCCCGCGACCTTGGCCCCGCCATGCTTTCTTAATGCCTCAGGCAGCATGACCACCT
  GCTTACACGAGCGCTCACGAGGACAAAGTCGTAAAATCCACTCCAGACTGTGTTCTAAGGGGGGCTTTAC
  TGTATTTGTTAATTAGTGTCACAAGCCAAGTGGTTCAATTTCATTCTTTATTTTTAACTTGACTACCTGT
  GATGACTTTGAGACTTAGAGTTCAGTTAAACCGATTATATTAGCTCTTAATTTTGCCAAAAATAGCTGGA
  CACATAGAAGAAATTATTTACTCTTTGATAAGTATATTCTGATATTTAAATGAAAATACTGTATTATTTA
  GTTTATTCCGTTAATTTAACTAGGAAAGATGCTATCTAAATTTAATAGGCTAGTGTGCTTGTGTGCTTTA
  ATTAAGTACTATTTTGTTAGTTACTGGTATGCATTGAAATATTTTCCCCCAAAGTAAGAACTGAAGCACA
  TTACAAAGGACCTAAGTGAAAGAGATTAAAATGAAAATTTTGTGAGGAACAATATGTTAAACTTTCTTGT
  TTGTTACTGTGCTGACATTTTGTAGAATTTTTTTTAAAAATCTGTCTAGTGGGTTATTTTGTGACACACT
  GGGGAAGTCAAAATAAATGTAAAATGAAAGAGATAAACTCATACTGTAGTCAGCAAGTCATGTTAGAGAT
  ACAGCAGAAAGGAGAATAACTACTCTAATTGTTACAATAGCCTTCTTTCCTCATTTGCCATTCTTCCATC
  CTCAGTATTTTTTACCCTAACATGGTTCTTCACATCTTCCCTTTTTCATTTGTGTTTTAACCACTTAGTT
  TTTCATTAGTCTTTGACTTTGTTTTCGTTATGAAAGTTTAAATTGGTCTCCATCCTTGGGCAGATTGATG
  GTTGCTTTTAAGGAAATCAAGCTCCATAGTCAGTGTTTTGTATGTATCTTAGGAAAATAATTTCAGATTT
  TAATAATTTTATCAAAGTGGTCTCCTTATAAATTCTTCACAGTGCAAACTTAAAGTGAAAATATTTTTAT
  TTCTCTCATTTTATTTACCTTTCTTTACATTTTCTATATCATAGATTATATAATATGTCAAGTTATTTTA
  TTTGTTCTACTAAATTTTCTAGCAGTTTCCATAGCCTCATCTCAGATTCACTTGTTGCAACCTATAAATA
  AGGACGTCTTTATTTTGCAAGTAAACTTAATTAAAAAGGGAAAAGTTGAAAAATTAAAAGACTAATAATT
  TAGAAATATCTACTTAATGTTCAGTTTGTCTCAATTCTTACTCATTTTGTGCCATTCCTTGTTTTATTAC
  AAAATGTTTTCTTCAAAAAGTACAAAAATGAAAAATGTGAGTACTTTTTGGTGCATTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001135044.1
• Summary: 'The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]'
• Locus ID: 5601