CXCR2 (NM_001557) Human 3' UTR Clone

CAT#: SC214255

3`UTR clone of interleukin 8 receptor beta (IL8RB) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CXCR2
• Synonyms: CD182; CDw128b; CMKAR2; IL8R2; IL8RA; IL8RB
• ACCN: NM_001557
• Insert Size: 1399 bp
• Sequence Data:
  Insert Sequence

  >SC214255 3'UTR clone of NM_001557
  The sequence shown below is from the reference sequence of NM_001557. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TTCTTCAGGGCACACTTCCACTACTCTCTAAGACCTCCTGCCTAAGTGCAGCCCCGTGGGGTTCCTCCCT
  TCTCTTCACAGTCACATTCCAAGCCTCATGTCCACTGGTTCTTCTTGGTCTCAGTGTCAATGCAGCCCCC
  ATTGTGGTCACAGGAAGTAGAGGAGGCCACGTTCTTACTAGTTTCCCTTGCATGGTTTAGAAAGCTTGCC
  CTGGTGCCTCACCCCTTGCCATAATTACTATGTCATTTGCTGGAGCTCTGCCCATCCTGCCCCTGAGCCC
  ATGGCACTCTATGTTCTAAGAAGTGAAAATCTACACTCCAGTGAGACAGCTCTGCATACTCATTAGGATG
  GCTAGTATCAAAAGAAAGAAAATCAGGCTGGCCAACGGGGTGAAACCCTGTCTCTACTAAAAATACAAAA
  AAAAAAAAAAATTAGCCGGGCGTGGTGGTGAGTGCCTGTAATCACAGCTACTTGGGAGGCTGAGATGGGA
  GAATCACTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATTGTGCCCCTGCACTCCAGCCTGAGCG
  ACAGTGAGACTCTGTCTCAGTCCATGAAGATGTAGAGGAGAAACTGGAACTCTCGAGCGTTGCTGGGGGG
  GATTGTAAAATGGTGTGACCACTGCAGAAGACAGTATGGCAGCTTTCCTCAAAACTTCAGACATAGAATT
  AACACATGATCCTGCAATTCCACTTATAGGAATTGACCCACAAGAAATGAAAGCAGGGACTTGAACCCAT
  ATTTGTACACCAATATTCATAGCAGCTTATTCACAAGACCCAAAAGGCAGAAGCAACCCAAATGTTCATC
  AATGAATGAATGAATGGCTAAGCAAAATGTGATATGTACCTAACGAAGTATCCTTCAGCCTGAAAGAGGA
  ATGAAGTACTCATACATGTTACAACACGGACGAACCTTGAAAACTTTATGCTAAGTGAAATAAGCCAGAC
  ATCAACAGATAAATAGTTTATGATTCCACCTACATGAGGTACTGAGAGTGAACAAATTTACAGAGACAGA
  AAGCAGAACAGTGATTACCAGGGACTGAGGGGAGGGGAGCATGGGAAGTGACGGTTTAATGGGCACAGGG
  TTTATGTTTAGGATGTTGAAAAAGTTCTGCAGATAAACAGTAGTGATAGTTGTACCGCAATGTGACTTAA
  TGCCACTAAATTGACACTTAAAAATGGTTTAAATGGTCAATTTTGTTATGTATATTTTATATCAATTTAA
  AAAAAAACCTGAGCCCCAAAAGGTATTTTAATCACCAAGGCTGATTAAACCAAGGCTAGAACCACCTGCC
  TATATTTTTTGTTAAATGATTTCATTCAATATCTTTTTTTTAATAAACCATTTTTACTTGGGTGTTTAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001557.3
• Summary: 'The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]'
• Locus ID: 3579