GLRB (NM_000824) Human 3' UTR Clone

CAT#: SC214262

3`UTR clone of glycine receptor beta (GLRB) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GLRB
• Synonyms: HKPX2
• ACCN: NM_000824
• Insert Size: 1381 bp
• Sequence Data:
  Insert Sequence

  >SC214262 3'UTR clone of NM_000824
  The sequence shown below is from the reference sequence of NM_000824. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCTGCTTCTTGTTCTTCAATGTTATATATTGGTCTATATATTTATGATAAATCTTTTCCATTTGTACAAA
  ATAAAATTCCATTTCATTGTGACCTACTCCTTTCATAAATGCCAATCTGTGAGAACTTTTGAATTTTCAT
  AGCAACATTGCATTTTGGATGCCATTTGATTGTAATAAAACTGTGGCACCTTAATTTTGAATGGCAGCAT
  GATCATGTAATATCTGTGCTCTAATAACGATGTATATATGTATAGTGAACATATTGCTTAGTAACAAATG
  AAGGACAAGCATACTACATAATATAATCCATACAATTCTCTTCAGTTAGTGTAAACTGCAAATACTACAG
  ATAATTCTGATAATAAAATGATATGCACGCTGAATCCTGCTATGGTCACCATTCTAATGTATGTAGTATT
  TCAAATTTCCTTCCTTGTAACTTTCAAAGAAAGCCATCTTATTCTTGTAAAATTTTAGATGGTATTATCA
  CAGATTTAAAAAGGTTGTATTACATATTGTTTAAACTTTGTAAGTAGAAATATATCTGTTATAATTATAC
  AGGCTCTGTGGAGAAATAAAGTTCAAAATATATTAATTTGTAAAATCAGCTCGTTTTAAAGTGTGCTTGT
  GTTGTCAAAAATATCAGATAGTAATACACAGTGAGCATTTTTAAACAAAGGGAAACCTATATTTATGTAA
  CTGTATACTGAATTCTGACAAAATAAAAAAAGATACCTTATTGACGAAATATTTAGGATAAACAAAATTC
  TATTTAATCCACCTTAAAACCTAAATGTATTTTCATGGATTTCATTTGTTGGTACATATTACACAAAACA
  TTGTGCCTTAAAATGAGTCATACATCTTTTAAATTGGAATGCAGTAATAGATATGTGATTTTACATCATT
  TTTAAGAAACCAAGGGGAAGTAATAAGTTGAAAAAGAAATCCATAACTATTAAAAGATTTTAACTTTTTT
  ATTTTATTAAAATGCTTGCATATTTTAAGTAAAATTAAAAATGTTTACTGAATTTATTTTTTTATTTGAA
  TATTTTGGGATTAGTTACAAAATATTTAGAGATTAAATAAATAATAGCTTTGTTTGTGTTAGACTTCACT
  TCTACTTGTATTTTCTTTCTTGTTAAAAGTCTAGACAACTGAGATTTACTTACTTTGAACTTGTTCCAAT
  CCAAAAGTAAGCACTAAGTCTCATTTTATGAGACCACCATTTCTTAATATCACATCCAGTGCACCTTTGT
  CTTTCTGCCATATCTGAAATAAAACTATCAGTAATTCACATAGATAAACATAAGACTAAAGAAGTATATT
  TACATTATCTGGAGAGTTTTGTTGCAGCTACGAGTTTGTATGGCAAATTCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000824.4
• Summary: 'This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. Mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]'
• Locus ID: 2743