Complement C7 (C7) (NM_000587) Human 3' UTR Clone

CAT#: SC214316

3`UTR clone of complement component 7 (C7) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: C7
• ACCN: NM_000587
• Insert Size: 1387 bp
• Sequence Data:
  Insert Sequence

  >SC214316 3'UTR clone of NM_000587
  The sequence shown below is from the reference sequence of NM_000587. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCTGTCACCAGCATAAGGCCTTGTGCTGCGGAAACCCAGTAGGCTCCTGGAGGCCCTGGTCAGCTTGCTT
  GGAATCCAGCAGGCAGCTGGGGCTGAGTGAAAACATCTGCACAACTGGGCACTGGACAGCTTTTCCTTCT
  TCTCCAGTGTCTACCTTCCTCCTCAACTCCCAGCCATCTGTATAAACACAATCCTTTGTTCTCCCAAATC
  TGAATCGAATTACTCTTTTGCCTCCTTTTTAATGTCAGTAAGGATATGAGCCTTTGCACAGGCTGGCTGC
  GTGTTCTTGAAATAGGTGTTACCTTCTCTGGGCCTTGGTTTTTTAAAATCTGTAAAATTAGAGGATTGCA
  CTAGAGAAACTTGAATGCTCCATTCAGGCCTATCATTTTATTAAGTATGATTGACACAGCCCATGGGCCA
  GAACACACTCTACAAAATGACTAGGATAACAGAAAGAACGTGATCTCCTGATTAGAGAGGGTGGTTTTCC
  TCAATGGAACCAAATATAAAGAGGACTTGAACAAAAATGACAGATACAAACTATTTCTATCCTGAGTAGT
  AATCTCACACTTCATCCTATAGAGTCAACCACCACAGATAGGAATTCCTTATTCTTTTTTTAATTTTTTT
  AAGACAGAGTCTCACTTTGTTGCCCAGGCTGGAGCGCAGTGGGGTGATCTCATCTCCCTGCAACCTCCGC
  CTCCTGGGTTCAAGCGATTCTTGTGCCTCAGCTTCCCAAGCAGCTGGGATTACAGGTGCCCGCCACCACG
  CCCAGCTAATTTTTGCATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCACGCTCGTCTCCAACTCCT
  GACCTCAGGTAATCCGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGACATGAACCACCACGCCTGG
  CTGGAATACTTACTCTTGTCGGGAGATTGAACCACTAAAATGTTAGAGCAGAATTCATTATGCTGTGGTC
  ACAGGGGTGTCTTGTCTGAGAACAAATACAATTCAGTCTTCTCTTTGGGGTTTTAGTATGTGTCAAACAT
  AGGACTGGAAGTTTGCCCCTGTTCTTTTTTCTTTTGAAAGAACATCAGTTCATGCCTGAGGCATGAGTGA
  CTGTGCATTTGAGAATAGTTTTCCCTATTCTGTGGATACAGTCCCAGAGTTTTCAGGGAGTACACAGGTA
  GATTAGTTTGAAGCATTGACCTTTTATTTATTCCTTATTTCTCTTTCATCAAAACAAAACAGCAGCTGTG
  GGAGGAGAAATGAGAGGGCTTAAATGAAATTTAAAATAAGCTATATTATACAAATACTATCTCTGTATTG
  TTCTGACCCTGGTAAATATATTTCAAAACTTCAGATGACAAGGATTAGAACACTCAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000587.2
• Summary: 'This gene encodes a serum glycoprotein that forms a membrane attack complex together with complement components C5b, C6, C8, and C9 as part of the terminal complement pathway of the innate immune system. The protein encoded by this gene contains a cholesterol-dependent cytolysin/membrane attack complex/perforin-like (CDC/MACPF) domain and belongs to a large family of structurally related molecules that form pores involved in host immunity and bacterial pathogenesis. This protein initiates membrane attack complex formation by binding the C5b-C6 subcomplex and inserts into the phospholipid bilayer, serving as a membrane anchor. Mutations in this gene are associated with a rare disorder called C7 deficiency. [provided by RefSeq, Nov 2016]'
• Locus ID: 730