UCK (UCK1) (NM_001135954) Human 3' UTR Clone

CAT#: SC214317

3`UTR clone of uridine-cytidine kinase 1 (UCK1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UCK1
• Synonyms: URK1
• ACCN: NM_001135954
• Insert Size: 1393
• Sequence Data:
  Insert Sequence

  >SC214317 3'UTR clone of NM_001135954
  The sequence shown below is from the reference sequence of NM_001135954. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCATCAACCTGATCGTGCAGCACATCCAGGACATTCTGAATGGTGACATCTGCAAATGGCACCGAGGAGG
  GTCCAATGGGCGGAGCTACAAGCGGACCTTTTCTGAGCCAGGGGACCACCCTGGGATGCTGACCTCTGGC
  AAACGGTCACATTTGGAGTCCAGCAGCAGACCCCACTGAGGGGCTGCCGAGCCTCAGGGCAGGTCTCCCG
  CCCGGCATGTGTGTTCAGGGACTGAGCCTGGGGACGCCCACCCACACCCACTGCTTCCTCTCGGCGCACC
  CCAGGGGAGTGTTAGCAGCGAGGCCTTCCTCACTCAGGAGTGGAAACTCAGATGTGTCACTCAGACTCAA
  CTTGCTGGGACACTGACAGGCGTTCCTGAGGTTTTCAGCCACTTAGGCTCGTTGCGGTTTAAAGATCCCT
  CTAGGTCACTGAGAAATGCCACAGAATGTGCAGGAAGCCTGGGAGGCTTCTGTGAGGAATGTGAGGCACA
  TTATTGGGGAAATTGAGGAGACAGCCTAGACACTGGCTGGCCTGATGTTTTGTTGACAGTGAACCCACAG
  TGGGAGAGAGTTTTTTCCAGTCTGATCTGGTTCTTACACACTCACACACATAACTCAAAAGTTTTGTGAA
  CAAGTACTTTCCTTTTTTACATGTTACATGTCCTCATGTTTTCTGTTTTCTGTTTCATAACACAAGGCTG
  GTTGTGGCCTACAAACCTAATTTCATGACCCAGTGGTTTGCAGTCCAGCGTGGCCTACACGGATATGGGG
  AGCCACTGAGGGATGTTTTCCCCCCTTGCTTGTGCCTTAAAGGCAGAGAAGCGAGGCGGATGCCCTGGAA
  GCACCCAGCATCACACCCAGGCTTGTGCGGGGCCAGGCTGGGAGCCCATGCAGTAGGGCAGAAGGCAGCG
  GAGGCCAGGTCTGTCCCGGCTGGAGAACAGCGTCCACGCAGTCCTGCCTGGGGTCAGGCCCTCACTGACC
  CTCAGGGGAGCCCCCAAGGTGCTGTCTGTCTAGACAGGCTGTCCCACCCCAGGGTGGCTAGTGGCCATAT
  GCAGGGAATGGTGCTTCTGGCTGTGGCACGCACTGGATCACCCAGGTCCCAGCAGACATGGCCGCCCAAA
  GTCAGCAAGCCTCCTTTGTGCTATGTGGAGCTCACAGCCTCACATGTGAACACCCGTGTCTGGGTTGCCT
  GGGGTGATCCTCCCTCCTGCGTGGTGGCTGTCTCTGGAAAGCATCCCTTGCCGCTGCCATGGGCAGCCCC
  AGCCCCCGTCCGTCCAGGCTCACCCACAGTAGTGATGCAGACGTGACGTGGGGGAAGGGGGCTGAGCCCT
  GTGGCTGGGTTCTGACAACTGTAACGGTTTTGTCGAGCTTAGGCCCCTTTGGAGGGAGAATCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001135954.1
• Summary: This gene encodes a uridine-cytidine kinase that catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP) but not the phosphorylation of deoxyribonucleosides or purine ribonucleosides. This enzyme can also phosphorylate uridine and cytidine analogs and uses both ATP and GTP as a phosphate donor. Alternative splicing results in multiple splice variants encoding distinct isoforms. [provided by RefSeq, May 2012]
• Locus ID: 83549