VR1 (TRPV1) (NM_018727) Human 3' UTR Clone

CAT#: SC214359

3`UTR clone of transient receptor potential cation channel subfamily V member 1 (TRPV1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TRPV1
• Synonyms: VR1
• ACCN: NM_018727
• Insert Size: 1378 bp
• Sequence Data:
  Insert Sequence

  >SC214359 3'UTR clone of NM_018727
  The sequence shown below is from the reference sequence of NM_018727. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAGGTCTTCAAGAGTCCTGCCGCTTCCGGGGAGAAGTGAGGACGTCACGCAGACAGCACTGTCAACACTG
  GGCCTTAGGAGACCCCGTTGCCACGGGGGGCTGCTGAGGGAACACCAGTGCTCTGTCAGCAGCCTGGCCT
  GGTCTGTGCCTGCCCAGCATGTTCCCAAATCTGTGCTGGACAAGCTGTGGGAAGCGTTCTTGGAAGCATG
  GGGAGTGATGTACATCCAACCGTCACTGTCCCCAAGTGAATCTCCTAACAGACTTTCAGGTTTTTACTCA
  CTTTACTAAACAGTTTGGATGGTCAGTCTCTACTGGGACATGTTAGGCCCTTGTTTTCTTTGATTTTATT
  CTTTTTTTTGAGACAGAATTTCACTCTTCTCACCCAGGCTGGAATGCAGTGGCACAATTTTGGCTCCCTG
  CAACCTCCGCCTCCTGGATTCCAGCAATTCTCCTGCCTCGGCTTCCCAAGTAGCTGGGATTACAGGCACG
  TGCCACCATGTCTGGCTAATTTTTTGTATTTTTTTAATAGATATGGGGTTTCGCCATGTTGGCCAGGCTG
  GTCTCGAACTCCTGACCTCAGGTGATCCGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAG
  CCTCCACACCTGGCTGTTTTCTTTGATTTTATTCTTTTTTTTTTTTTCTGTGAGACAGAGTTTCACTCTT
  GTTGCCCAGGCTGGAGTGCAGTGGTGTGATCTTGGCTCACTGCAACTTCTGCCTCCCGGGTTCAAGCGAT
  TCTTCTGCTTCAGTCTCCCAAGTAGCTTGGATTACAGGTGAGCACTACCACGCCCGGCTAATTTTTGTAT
  TTTTAATAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCTTGACCTCAGGTGATCTGCC
  CGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCGCTGCGCTCGGCCTTCTTTGATTTTATAT
  TATTAGGAGCAAAAGTAAATGAAGCCCAGGAAAACACCTTTGGGAACAAACTCTTCCTTTGATGGAAAAT
  GCAGAGGCCCTTCCTCTCTGTGCCGTGCTTGCTCCTCTTACCTGCCCGGGTGGTTTGGGGGTGTTGGTGT
  TTCCTCCCTGGAGAAGATGGGGGAGGCTGTCCCACTCCCAGCTCTGGCAGAATCAAGCTGTTGCAGCAGT
  GCCTTCTTCATCCTTCCTTACGATCAATCACAGTCTCCAGAAGATCAGCTCAATTGCTGTGCAGGTTAAA
  ACTACAGAACCACATCCCAAAGGTACCTGGTAAGAATGTTTGAAAGATCTTCCATTTCTAGGAACCCCAG
  TCCTGCTTCTCCGCAATGGCACATGCTTCCACTCCATCCATACTGGCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_018727.5
• Summary: 'Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 7442