CEBP Alpha (CEBPA) (NM_004364) Human 3' UTR Clone

CAT#: SC214412

3`UTR clone of CCAAT/enhancer binding protein (C/EBP) alpha (CEBPA) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CEBPA
• Synonyms: C/EBP-alpha; CEBP
• ACCN: NM_004364
• Insert Size: 1418
• Sequence Data:
  Insert Sequence

  >SC214412 3'UTR clone of NM_004364
  The sequence shown below is from the reference sequence of NM_004364. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAGAGCTCCTTGGTCAAGGCCATGGGCAACTGCGCGTGAGGCGCGCGGCTGTGGGACCGCCCTGGGCCAG
  CCTCCGGCGGGGACCCAGGGAGTGGTTTGGGGTCGCCGGATCTCGAGGCTTGCCCGAGCCGTGCGAGCCA
  GGACTAGGAGATTCCGGTGCCTCCTGAAAGCCTGGCCTGCTCCGCGTGTCCCCTCCCTTCCTCTGCGCCG
  GACTTGGTGCGTCTAAGATGAGGGGGCCAGGCGGTGGCTTCTCCCTGCGAGGAGGGGAGAATTCTTGGGG
  CTGAGCTGGGAGCCCGGCAACTCTAGTATTTAGGATAACCTTGTGCCTTGGAAATGCAAACTCACCGCTC
  CAATGCCTACTGAGTAGGGGGAGCAAATCGTGCCTTGTCATTTTATTTGGAGGTTTCCTGCCTCCTTCCC
  GAGGCTACAGCAGACCCCCATGAGAGAAGGAGGGGAGCAGGCCCGTGGCAGGAGGAGGGCTCAGGGAGCT
  GAGATCCCGACAAGCCCGCCAGCCCCAGCCGCTCCTCCACGCCTGTCCTTAGAAAGGGGTGGAAACATAG
  GGACTTGGGGCTTGGAACCTAAGGTTGTTCCCCTAGTTCTACATGAAGGTGGAGGGTCTCTAGTTCCACG
  CCTCTCCCACCTCCCTCCGCACACACCCCACCCCAGCCTGCTATAGGCTGGGCTTCCCCTTGGGGCGGAA
  CTCACTGCGATGGGGGTCACCAGGTGACCAGTGGGAGCCCCCACCCCGAGTCACACCAGAAAGCTAGGTC
  GTGGGTCAGCTCTGAGGATGTATACCCCTGGTGGGAGAGGGAGACCTAGAGATCTGGCTGTGGGGCGGGC
  ATGGGGGGTGAAGGGCCACTGGGACCCTCAGCCTTGTTTGTACTGTATGCCTTCAGCATTGCCTAGGAAC
  ACGAAGCACGATCAGTCCATCCCAGAGGGACCGGAGTTATGACAAGCTTTCCAAATATTTTGCTTTATCA
  GCCGATATCAACACTTGTATCTGGCCTCTGTGCCCCAGCAGTGCCTTGTGCAATGTGAATGTGCGCGTCT
  CTGCTAAACCACCATTTTATTTGGTTTTTGTTTTGTTTTGGTTTTGCTCGGATACTTGCCAAAATGAGAC
  TCTCCGTCGGCAGCTGGGGGAAGGGTCTGAGACTCCCTTTCCTTTTGGTTTTGGGATTACTTTTGATCCT
  GGGGGACCAATGAGGTGAGGGGGGTTCTCCTTTGCCCTCAGCTTTCCCCAGCCCCTCCGGCCTGGGCTGC
  CCACAAGGCTTGTCCCCCAGAGGCCCTGGCTCCTGGTCGGGAAGGGAGGTGGCCTCCCGCCAACGCATCA
  CTGGGGCTGGGAGCAGGGAAGGACGGCTTGGTTCTCTTCTTTTGGGGAGAACGTAGAGTCTCACTCTAGA
  TGTTTTATGTATTATATC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_004364.3
• Summary: This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]
• Locus ID: 1050