Collagen IV alpha 6 (COL4A6) (NM_001847) Human 3' UTR Clone

CAT#: SC214469

3`UTR clone of collagen type IV alpha 6 (COL4A6) transcript variant A for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: COL4A6
• Synonyms: CXDELq22.3; DELXq22.3; DFNX6
• ACCN: NM_001847
• Insert Size: 1399 bp
• Sequence Data:
  Insert Sequence

  >SC214469 3'UTR clone of NM_001847
  The sequence shown below is from the reference sequence of NM_001847. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCAGTCGCTGCCAGGTGTGTATGAAAAGCCTGTAGGGTGGCACCTGCCACTCTGCCCCTTGCCCTCCCCT
  GCCCCTCACAACAGTCACCTCACAAACCTGAATGGTCTGAAGAAGGAAGGCCTGAGCCCCTTTGCCTGTC
  AAGTTGTACATTGGAGTCTCATTTGGGCTAGACTACCGGACACTCGTCACCCCAGCCCTCGGGTCCATAG
  AGATGAGCCCACCCTGCTGAGATCTGCTGTCCTGTTTCTGTCAAGCTGGTGCTACTGTTTGATTTGGATG
  ATTGTGTGACTATTCATGGCTACCTCAGAAAGATTTGATGGGCCACAACTGTCTTAGACTGCTAGCTTTC
  TCCTTACCGTCTTGATCGGAAAGCTCTTCCTAATCGCTAATCAGTCATTTCTTCATGTACAGAGGTCAGC
  ACACATTATTTGGCTTAAACCAGAACCCAGTGTTTCCACACTTAAATTCTCTAACCGAATATTCATGGAT
  GGCTCAAGTCTGCACAGAGCAAGTCCTCACTCTTCAAGGAGGCCCACTGTGTCTAGGCAGGCAAGAGAAT
  TGAAATGAGGTGCCACCCAGTAGCCCAGAGTGAGCTTTAGCTCTCTCTAGAATGAGCAAGACTGGGCCCC
  ACATGGCTTAGAGAGGCTTGAAGGCCAGCAGCTGGGTTGGGGGTGGTGGTCATTAATGGCATATGGTCCT
  AGACAAACCATCTCCTCCTTGCCGGCTCCCCCTCCAGCCAGAGACAGAGGATGTGGCCTGGTTCAAAGTA
  AAGCAGAGGATGCAACAAATGTGGCCAAGCCTATCAAAGGAAATGAGAATGACAGCCTTTTTTCCTGGGC
  CAGAAGTAGAGGGGTGGGTGCGTAAGGATGTGTGAGTTTTGCTTTTGACTCCAGGAACAAAAAGGTAAAT
  CCCACATCCCAGTTTCTCAGAAGTCCCTGTTTATTCCAAATGCCATCCAGATGTGTGCAATGTGGCAAAC
  TGAAGCTGCACAGTGTTGGTTTCCTTGTATTCTGAGGATGTTAAAGACTTTGTTAAATGGTTATCCAATT
  GCTCTTTCACAGGTAGCCTATTAAACTATTTTAATATGTTTTTTTAAACCTCATAAAAATCTAGCACACT
  CTTCTCTTGAGCAGTTAGCAGACCTAAAGCAAGCCTGAATTGGCTATGCAGTACATTGTATTCTGTTTGG
  GGGAATTTGTTTTAGCCATTTTCTTTAATTACCAGTTTTCCAGAACACTCTTAGCTATGTTGACATGAGG
  CAGTTCCTTCCAGGTGATTCTGTTTCCTTAAGTATTATATAAACTGTGCCAATACAGACAAAGCATAATC
  AATATAATCTGAATTATTGTTATCTTTACCTCCTGAGTAATAAGCATGGTGTCAGTTTTGTACATAGCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001847.2
• Summary: 'This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]'
• Locus ID: 1288