POLR1B (NM_019014) Human 3' UTR Clone

CAT#: SC214609

3`UTR clone of polymerase (RNA) I polypeptide B 128kDa (POLR1B) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: POLR1B
• Synonyms: RPA2; RPA135; Rpo1-2
• ACCN: NM_019014
• Insert Size: 1457
• Sequence Data:
  Insert Sequence

  >SC214609 3'UTR clone of NM_019014
  The sequence shown below is from the reference sequence of NM_019014. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGGCAGCTATGAACATCAAAGTGAAACTGGATGTTGTTTAACTTGATGTTGACCTTTTGGATTAAGAGGA
  CTATCAGATTAAAGCAAAATGTAATTTTAATTCAATGAAGATATCATTACCAGGTTACTCTTGAGATTTT
  TCAACGGTGTTAGAACTCTCAACCAAGACCTGAAAACCAAGTATGCAAGGTTTCTGAATCTCTCTGGTAG
  ATTAACTATTGACAATGATTTTCTGTTATCTTTGTTCAAAAAGTTCATGTCTTCTCAAAATATGAAATAT
  TGATAAATGGAAGAGCATACGGTGACAAGTCTCCTTTCCAACCCCAGGTTCCCTACACCCTGCTCTCAGC
  AGGCAGTGAGTGTCACACACCTGTTAATCCATCTTGAGCAGGACAGTACTATACAAATAGAATGCAAGCT
  GTAATGTAATTTTATATTTTCTTATAGCCACGTTGAAGTAAAAACAAACAGGTACAGTGTTTTTTACCAG
  CTTTATAGAAGTACAGTTGTTACATATTTAATGAATACAATTTGATGGGTCTGACTATATGCACACACCT
  TTGATACCATCACCACAATCAGGGTAATAAACATACCTGTCATCTCCACAAGTTTCCTCCTGCCCCTTTG
  TTTTTTGCTTTTTGGTTGCTGTTGAGTTTTTGTTTTGTCTTCTGTGGTAAGAACACTTAACTCAAGACCT
  ACCCTCTTAACAAATCTTTAAGTGCACGATATAGTATTGTTAATTCCAGGCACCATGTTGTACAACAGAT
  CTTTAGACCTTACTTGTCTTGCATAACTGAAGCTTTATACCTGTTGAACAACTCTCCATTTCCCTGGCCC
  CTAGCAACCACCCTTCTACCCTGTTTCTATGAGTTTGACTATTACAGATATCTCATATAGTGGGATCATG
  CAATATTTGTCCTGTGACTGGCTTATTTCACTTAGCATAGTGAAATAAGATTCATCCATTTTGGAAGCCA
  GGCATGGTGCTGTGCATCTATAGTCCCTGCTATTTGAGAGGCTGAGGTGGGAGGATCATTTGAGTGCAGG
  AGTTCAAGGACAGCCTGGGTAATATAGGAAGACCCTGTCTTGAAGACCCTGACCTCAAGTGATCCACCCA
  CCTCGGCCTCCGAAAGTGCTAGGATTACAGGTGTGAGCCACTGTGCCTGGCCTCCGGTGAGTATTTTATA
  TTTAGTCTACACTTCCATACTTGGCTTTTTTCTGCTTTTATATTGATCTGCTTTCATAGCAGTGTGTAGA
  GTGCCACTTATGTTTTCTTTCTTGTGTACAGTATTTTATTGTATGGATTTACCATCCCCTGTGTATTTAA
  GTTGTTCCATTCTTTGGCCATTATAACTTTTTTCTGCAAATATTCTGGTGACTTATCTTTGGCCATTATA
  AACTGTTGATAATAGATCATCTTGTATATACTTCTGCAATTATAAGATGTTTTTTGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_019014.4
• Summary: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]). [supplied by OMIM, Mar 2008]
• Locus ID: 84172