Cortactin (CTTN) (NM_005231) Human 3' UTR Clone

CAT#: SC214697

3`UTR clone of cortactin (CTTN) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CTTN
• Synonyms: EMS1
• ACCN: NM_005231
• Insert Size: 1442 bp
• Sequence Data:
  Insert Sequence

  >SC214697 3'UTR clone of NM_005231
  The sequence shown below is from the reference sequence of NM_005231. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAGCCAACTATGTGGAGCTGCGGCAGTAGGGCCCCCAGCCCCCCCCCGGAGCTGCGCCCTGGATCCTCAC
  ACTACAGATCAGGCCTTCTTTGGTTCTTGGGTGGTTTTGGGTTTTTTCTGTTTTTTTTTTTTTTTTTTTT
  TTTTTGAAGGTGGGGAGGGGAATATACACATTGCTTTTATATTTAATACTTTTGCTGATGCTTTTGAAAA
  TGTTTATGCCACAGAATTTGCTAATATATTGTAATCACATTCCTTAGGAGGACTTTGGTAATTGGTTTTA
  TGCATTGATGGTTTTTTTTTTCTTTTTTGCCAAATTGACTGTCACGCGGCAGCTTCAGGGAGCTCGCATT
  CTCTTGTGTTCGTGTTGCCCTCGTGCCCATCAAGTGCAGTCGGGACCTCCCAGGACAAGCACGAGGCCTC
  AGGTCGGCCCTGTGGCGGGTAGGCAGGAAGGACTGTCCCAGACGAGGGGCTTCCTCTAGAGTCTCACTGC
  TGGGGAGGAGAGGACTGGGCCTGATGGAAGTTAACCCGGAGCTAAGTCACCCAGAGCACAGGAGCTGCCA
  TGTCAGATGGGAAATCTGCCTATGTCATACCGTGACAGCCCGCAGGATCAGGTGACTTCTAGCAGAGACC
  CTGGTTTTTTTCCTGTGCCCACTCCGGCTTGTCCTCATCTCTACCCATCCCCTGATGCCCAGGTCACCGG
  GAGGGCTGCTGGGAGCCTCTCCTGTCCCCGCCGGGCAGTGTCACTGAGTCCTTGAAATCCTCCCCTGCCC
  CGCGGGTCTCTGGATTGGGACGCACAGTGCAGTTGAGGTCTGCGTCGGGCTTGGCTTTTCACAAAGGCTG
  ATGTCTTAACTGTCACCCATATGGTCCCTGGGCCACCGGGCAGCCTGGGGCGGTGTGTGTGCCATGTCAC
  AGCATGGCCTCTCGGCCTTGGGAAGGAAGGCAGTGCCTGCTCTGCTGTGAGCCGCCAGGAACCCTCCTCC
  TGTCAATGGGGGTGTAGTATTTTTGCCAAAATATCATGTTCAATTTCAGTAGTTTGATCAGTTGAAGGCT
  AGAAGTGTGAAGTGCAGATGAGTGTGTGTTCTTCCCCAAGGTCCCCCCACAGCTCCAGGACACCGCTGTC
  CTGGCATTTGTGGCCACTCACTTTGTAGGAAACTCATCTCCTTCCTGAGGAGCCGGGAGGCTGGACCAGT
  CCCGTCGTGCAGTCAGGTGGGCGGTGTGTCTTTCCAGAAGGTCACGTGGAAATGTCTCGGGACTTGGGTC
  CCGGAGTGCCCGTGAAGCGTGTTTTTGCTCCTGAGGTGCATTTTCTCATCATCCTTGCTTTACCACAATG
  AGCAATGAGGTCGGGTTTTATATGCAACTTATTGTATCTGAATTCCTGTAGCACACCTCATAGGTATGAT
  TTTTTTAAATTAAAGAATTCAGAATAAACATTTTTTGATCCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005231.3
• Summary: 'This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]'
• Locus ID: 2017