SAE1 (NM_001145714) Human 3' UTR Clone

CAT#: SC214830

3`UTR clone of SUMO1 activating enzyme subunit 1 (SAE1) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SAE1
• Synonyms: AOS1; HSPC140; SUA1; UBLE1A
• ACCN: NM_001145714
• Insert Size: 1508
• Sequence Data:
  Insert Sequence

  >SC214830 3'UTR clone of NM_001145714
  The sequence shown below is from the reference sequence of NM_001145714. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGGAGGGATTTTGGCACAGGAAATTGTGAAGGCCCTGTCTCAGCGGGACCCTCCTCACAACAACTTCTTC
  TTCTTCGATGGCATGAAGGGGAATGGGATTGTGGAGTGCCTTGGCCCCAAGTGAACTCAAGATTTGGCAG
  CCCCAGAGATGCCAACTGCAGCATGCCCACCTGTATTCCCTGTCCCCTTCCTTCATGAAGGCATCTCCAG
  GCAAGGAAAACTGAAGTCATTGGCCCGATACAAAACATTTCCTGCAACGAAGGAGGTGGTGCCGACGTGC
  TGCTTCCCATCACCAGCAGCTGCTCGACAAGGGGCGCAGGGTGGCTGTCTTTGTTCCAGCACTGTTCAGG
  CTGCCTGTCATCCCGGGCCTGCCAGCTCCCCTGAGTGATGAGCACTTCCAAGCACCCCTCTGCCCTTTCT
  CTGTCCTTATGCTGTCCCGGCCTCGCCAGCCCTCTGGGGCATTGTGGGAGATGCCTGCCAGGAATGAGCA
  AGCTCTGTTGCTCGGGAGCCTCTTGTCACCTTCTTGGACTTATTCCCCACCTGATACCTTATAGAGAAAA
  GTGTGAATTCAGGTGGAGAGTAGGCCCAGGCCCCATGAGGCACCAGTGGAAGCACAGCTCCAAGTTCAGA
  CAGGTGCCCTTAGAGAGGAAAACCATGACAGGCAAATGCATTTCCTCTGGAGTTTGAGACCCTGACAAAC
  AACAGGTGGCATCTGGTGTGCTGTTCTTGAGTTTTCGTTTAGGATTAGTTGAGTTCCAGCTGGGTTTTGG
  GAGAAAGGAGATGCTACCAAGTCTTGGATGTTAGGGCGAGACCCTGCAAGTTGAGTATTAGAGAGCTTGT
  CTTTCAAGGCAGGTTCCTGGGGCTTCAGGGCTAGGAGGGAGGAGCCTGCCCTTTTAACAGAACCCCAGTC
  ACATGCGGCTCAAGTCACTCAGAGGCTGTTGCATTTCAGGGCTATGTTGGTCCTTTGTTTACCTCCTAAA
  CCACAGCTGTTTGTGTTTCACATATGTTGTGAATTTTCCTTGGTTCTTTTTAAAGGAATGATAATAAAGT
  TACTTGCTTTAGGATTTGCTTGTTTTTCTTCCACTTCAGAAGCTTCTGAGAGGGAATGGGATGATCCTAC
  CAGTTGCCTTTTCAGACCTGAGGCTCTAACTCAAGAGATTCCTCCTCTCCCTCACCATTCCTGCCACCAT
  TTTTTCTGGGTGATGCAGCAAGAGTTAAATTGTTCACATTCTAGAATGTGTAGAAGCTTCTGGCTCCAGT
  TGTCTAATGGATAATTCAGCTAAATAGTGGCTATTTTCAGTGGCAAGAATTATAATAATAAAGGGAAGTC
  AAAAGTGATGCTTCATATGCCCCAAGTGCCACAGCCGCTACGGGTCAGAGCTTTTATTGGAAGACCAGGT
  TTGCCTTGATGCAGCCAAAGTGCGTTCCAGTTCACCCCACTCTTGCTTGTGTTTCTTCTTTATGTAAATT
  GTGTGATGTTTCAATAAATCAGTCTCTTTCCTATTCTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001145714.1
• Summary: Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]). [supplied by OMIM, Mar 2010]
• Locus ID: 10055