Cytochrome P450 1A2 (CYP1A2) (NM_000761) Human 3' UTR Clone

CAT#: SC215008

3`UTR clone of cytochrome P450 family 1 subfamily A polypeptide 2 (CYP1A2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CYP1A2
• Synonyms: CP12; CYPIA2; P3-450; P450(PA)
• ACCN: NM_000761
• Insert Size: 1505 bp
• Sequence Data:
  Insert Sequence

  >SC215008 3'UTR clone of NM_000761
  The sequence shown below is from the reference sequence of NM_000761. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGCGCTTCTCCATCAACTGAAGAAGACACCACCATTCTGAGGCCAGGGAGCGAGTGGGGGCCAGCCACG
  GGGACTCAGCCCTTGTTTCTCTTCCTTTCTTTTTTTAAAAAATAGCAGCTTTAGCCAAGTGCAGGGCCTG
  TAATCCCAGCATTTTAGGAGGCCAAGGTTGGAGGATCATTTGAGCCCAGGAATTGGAAAGCAGCCTGGCC
  AACATAGTGGGACCCTGTCTCTACAAAAAAAAAATTTGCCAAGAGCCTGAGTGACAGAGCAAGACCCCAT
  CTCAAAAAAAAAAACAAACAAACAAAAAAAAAACCATATATATACATATATATATAGCAGCTTTATGGAG
  ATATAATTCTTATGCCATATAATTCACCTTCTTTTTTTTTTTTTGTCTGAGACAGAATCTCAGTCTGTCA
  CCCAGGTTGGAGTGCAGTGGCGTGATCTCAGCTCACTGCAACCTCCACCTCGCAGGTTCAAGCAATCCTC
  CCACTTCAGCCTCCCAAGCACCTGGGATTACAAGCATGAGTCACTACGCCTGGCTGATTTTTGTAGTTTT
  AGTGGAGATGGGGTTTCACCATGTTGGCCAGGCTTGTCTCGAACTCCTGACCCCAAGTTATCCACCTGCC
  TTGGCTTCCCAAAGTCCTGGGATTACAGGTGTGAGCCACCACATCCAGCCTAACTTACATTCTTAAAGTG
  TCGAATGACTTCTAGTGTAGAATTGTGCAACCATCACCAGAATTAATTTTATTATTCTTATTATTTTTGA
  GACAGAGTCTTACTCTGTTGCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTACAACCTCCGCCTC
  CCATGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTATAGGCATGCGCCACCATGGCC
  AGCTAATTTTTGTATTTTTAGTAGAGACGAGGTTTCACTGTGTTGGCCAGGATGGTCTCCATCTCTTGAC
  CTCGTGATCCACCCGCCTCAGCCTCCCAAAGTGCTGGGATTAACAGGTATGAACCACCGCGCCCAGCCTT
  TTTGTTTTTTTTTTTTTTGAGACAGAGTCTTCCTCTGTCTCCTAAGCTGGAGTGCAGTGGCATCATCTCA
  GCTCACTGCAACCTCTGCCTCCCAGGTTCAAGTGCTTCTCCAGCCTCAGCCTCCCAAGTAGCTGAGACTA
  CAGGCACACACCACCACGCCTGGCTAATTTTTGTATTTTTAGTAGAGACGGGTTTCACCATGTTGGCTAG
  ACTAGTCTCAAACTCCTGACCTCAAGTGATCTGCCCGCCTCGACCTCTCTCAAAGTGCTGGCATTACAGG
  TGTGAGCCACGGTGCCCGGCCCACAATTAATTTTAGAACATTTTCATCACCCCTAAAAGAAACCCTGCAC
  CCATTAGCAGTCCCTCCACATTTCCCCCTAGCCTGCCTCCCCTGCCTCACCAGCCCTGGCAACTGCTAAT
  CTACTTTCTGTGTCTATGGATTTGCCTTCTCTAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000761.3
• Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq, Jul 2008]'
• Locus ID: 1544