ALG2 (NM_033087) Human 3' UTR Clone

CAT#: SC215015

3`UTR clone of asparagine-linked glycosylation 2 alpha-13-mannosyltransferase homolog (S. cerevisiae) (ALG2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ALG2
• Synonyms: CDG1I; CDGIi; CMS14; CMSTA3; hALPG2; NET38
• ACCN: NM_033087
• Insert Size: 1545
• Sequence Data:
  Insert Sequence

  >SC215015 3'UTR clone of NM_033087
  The sequence shown below is from the reference sequence of NM_033087. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAGAACAGCTCTACCGATATGTTACCAAACTGCTGGTATAATCAGATTGTTTTTAAGATCTCCATTAATG
  TCATTTTTATGGATTGTAGACCCAGTTTTGAAACCAAAAAAGAAACCTAGAATCTAATGCAGAAGAGATC
  TTTTAAAAAATAAACTTGAGTCTTGAATGTGAGCCACTTTCCTATATACCACACCTCCCTGTCCACTTTT
  CAGAAAAACCATGTCTTTTATGCTATAATCATTCCAAATTTTGCCAGTGTTAAGTTACAAATGTGGTGTC
  ATTCCATGTTCAGCAGAGTATTTTAATTATATTTTCTCGGGATTATTGCTCTTCTGTCTATAAATTTTGA
  ATGATACTGTGCCTTAATTGGTTTTCATAGTTTAAGTGTGTATCATTATCAAAGTTGATTAATTTGGCTT
  CATAGTATAATGAGAGCAGGGCTATTGTAGTTCCCAGATTCAATCCACCGAAGTGTTCACTGTCATCTGT
  TAGGGAATTTTTGTTTGTCCTGTCTTTGCCTGGATCCATAGCGAGAGTGCTCTGTATTTTTTTTAAGATA
  ATTTGTATTTTTGCACACTGAGATATAATAAAAGGTGTTTATCATAAAAAAGAAACAGTATTAGATTTTG
  GTCTCCATAATCTATTTTGGTATTGTTACGAACATGGATATGACAACCAAACTGGAAATCAGAACACTAG
  GGTAAAGTGGATATTGAAATGAAGCAAGAATATTGTCACACATGTGTTGTGCATCTTGTTTAGGGTATAT
  TTCTTAATGTCATCTAGGTCATTAGTTTTGTTAATATTTGTGTTGTCTTGACCAAGCTCCTACTAAGTAT
  AGGACACAAATGTTTTTTATCTTCCAAGGCCTGGCTCAAATGCCACTGCTGCAAAGCTTTCTTTGACCCT
  CTGGCCACCTCCCAAGCCAGAAGTTATCTTCCCCCTCCATGTACTCTAGCCTTTTCATGACACTGATATT
  TTCGTGACACTGACTTATAGTTCACTGTTTACCTGTTGGTCTAACAGCAAATCATAAACAGTAGTTTTCA
  ACATACTTCTAAGGCTAAATTCATTTCCAGAAGTAGCAAAGCTTACTAGCCAAAGGCTCCACATTTATCA
  ACTCTTAAGAGCTAGACTTGAGAGCCTAACAGCCTATATGATTTTGTGTAAATTGATCTCTATACCTCAG
  CTTCTTCATCTGCAAAGTGAGATAATTGTACTATTCTACTTTCCTCATAGTGCGGTTATAACAGATTATG
  ATAATATTACAAAGTACTTAATACTTGGCATATAGTATATATATTAGCTATTAGTATTTTGCAATTTGGG
  GATATTGAAAATTAGAAATTGCATGTTAGTTCATGCTGCACTACCATATTGTTCTTTTGGGGCTCTTCAA
  TGGGTGAAATTCCATTTTACAGTTTATCATGTGCATCTGTATCACAGGAGTATTTTGGCAAAATGATTCT
  GTACCAAAAAGGAGTACTATAAAATGAGGGTGCCAAATATATTGAGTCTTTTATGGGATTAAAAGTTCTG
  TTGCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_033087.3
• Summary: This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]
• Locus ID: 85365