SFRP4 (NM_003014) Human 3' UTR Clone

CAT#: SC215219

3`UTR clone of secreted frizzled-related protein 4 (SFRP4) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SFRP4
• Synonyms: FRP-4; FRPHE; FRZB-2; PYL; sFRP-4
• ACCN: NM_003014
• Insert Size: 1551 bp
• Sequence Data:
  Insert Sequence

  >SC215219 3'UTR clone of NM_003014
  The sequence shown below is from the reference sequence of NM_003014. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCAGAAGAGAACAAACCCGAAAAGAGTGTGAGCTAACTAGTTTCCAAAGCGGAGACTTCCGACTTCCTTA
  CAGGATGAGGCTGGGCATTGCCTGGGACAGCCTATGTAAGGCCATGTGCCCCTTGCCCTAACAACTCACT
  GCAGTGCTCTTCATAGACACATCTTGCAGCATTTTTCTTAAGGCTATGCTTCAGTTTTTCTTTGTAAGCC
  ATCACAAGCCATAGTGGTAGGTTTGCCCTTTGGTACAGAAGGTGAGTTAAAGCTGGTGGAAAAGGCTTAT
  TGCATTGCATTCAGAGTAACCTGTGTGCATACTCTAGAAGAGTAGGGAAAATAATGCTTGTTACAATTCG
  ACCTAATATGTGCATTGTAAAATAAATGCCATATTTCAAACAAAACACGTAATTTTTTTACAGTATGTTT
  TATTACCTTTTGATATCTGTTGTTGCAATGTTAGTGATGTTTTAAAATGTGATCGAAAATATAATGCTTC
  TAAGAAGGAACAGTAGTGGAATGAATGTCTAAAAGATCTTTATGTGTTTATGGTCTGCAGAAGGATTTTT
  GTGATGAAAGGGGATTTTTTGAAAAATCTAGAGAAGTAGCATATGGAAAACTATAATGTGTCTTTTTTAC
  AATGACTTCAGCTCTGTTTTTAGCTAGAAACTCTAAAAACAAAAATAATAATAAAGAAAAATAAATAAAA
  AGGAGAGGCAGACAATGTCTGGATTCCTGTTTTTTGGTTACCTGATTTCATGATCATGATGCTTCTTGTC
  AACACCCTCTTAAGCAGCACCAGAAACAGTGAGTTTGTCTGTACCATTAGGAGTTAGGTACTAATTAGTT
  GGCTAATGCTCAAGTATTTTATACCCACAAGAGAGGTATGTCACTCATCTTACTTCCCAGGACATCCACC
  CTGAGAATAATTTGACAAGCTTAAAAATGGCCTTCATGTGAGTGCCAAATTTTGTTTTCTTCATTTAAAT
  ATTTTCTTTGCCTAAATACATGTGAGAGGAGTTAAATATAAATGTACAGAGAGGAAAGTTGAGGTTCCAC
  CTCTGAAATGAGAATTACTTGACAGTTGGGATACTTTAATCAGAAAAAAAGAACTTATCTTGCAGCATTT
  TATCAACAAATTTCATAATTGTGGACAATTGGAGGCATTTATTTTAAAAAACAATTTTATTGGCCTTTTG
  CTAACACAGTAAGCATGTATTCTCTATAAGGCATTCAATAAATGCACAACGCCCAAAGGAAATAAAATCC
  TATCTAATCCTACTCTCCACTACACAGAGGTAATCACTATTAGTATTTTGGCATATTATTCTCCAGGTGT
  TTCTTATGCACTTATAAAATGATTTGAACAAATAAAACTAGGAACCTGCTATACATGTGTTTCATAACCT
  GCCTCCTTTGCTTGGCCCTTTATTGAGATAAGTTTTCCTGTCAAGAAAGCAGAAACCATCTCATTTCTAA
  CAGCTGTGTTATATTCCATAGTATGCATTACTCAACAAACTGTTGTGCTATTGGATACTTAGGTGGTTTC
  TTCACTGACAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003014.3
• Summary: 'Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]'
• Locus ID: 6424