DCK (NM_000788) Human 3' UTR Clone

CAT#: SC215224

3`UTR clone of deoxycytidine kinase (DCK) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DCK
• ACCN: NM_000788
• Insert Size: 1550 bp
• Sequence Data:
  Insert Sequence

  >SC215224 3'UTR clone of NM_000788
  The sequence shown below is from the reference sequence of NM_000788. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AAGTCTGGTTGAAAAGGTCAAAGAGTTTTTGAGTACTTTGTGATCTTGCTGAAGACTACAGGCAGCCAAA
  TGGTTCCAGATACTTCAGCTTTGTGTATCTTCGTAACTTCATATTAATATAAGTTTCTTTAGAAAACCCA
  AGTTTTTAATCGTTTTTGTTTTAAGGAAAAAAGATTTTTAAAATGAATCTTATGCAAAACTTTTTGACCA
  GTTTCTTTTCTTTTGTTTTTTTTTTAAAAAAGACATTTAAAGACAAAGACATTATTTCTCATAGCAGGAA
  ATGTAGAGGTAGATGGTTCCAGTATCAGCATAGTGACTAAACTACATTATAAAAGATCCAGCTTCCTTCT
  GTCATTCCCCTCTTTTGTCTTCCTCAGCAGGTTGGCTTTTTTCCCTGGTGCCTCTCACTTCGTTGGTGAC
  CAGTTTCTTAAACTGAAAGCTTTAATGTTACATAGTAAATGGTAGTGTGTCCTGTGTAAATTAGTGTACC
  TATTAAAAGTTGCAAAGTGGAATTAAAGGAATCCCTAGAATAAGGATTCTGAAGTTTTATTTTAAATTAT
  TATCTTCTTAACAGTTTAGTCCCACCTCTTACTTCCTGCCTCAGTCTGCTTTCTCTACTGTCTGGATTAA
  TTAGGCAGCCTGCTATAAAGTTAAAGTCACACATTTCTATTTTGCAAACACTGTGATTACTCTTTGCTTT
  GTAGTTTGCTTTGCTTTGTAGGGTTCTGCTTTTAAGTTTTTCTCTTTTTCAGACAAATTACTGATAAAAA
  TGATATTGCTCTATATGTAATATATCCTGAAAGCATTATTTTTTGTTGAATAGGAAATAAAATTAATGAA
  GACAGAGGCTAGAAAGCATCCATTAATTAATGAGACACACTTAACTACTTATCTCTAAACCATCTATGTG
  AATATTTGTAAAAATAATGAATGGACTCATCTTAGTTCTGTATATAAATATATTTTCTTTCTAGTTTGTT
  TAGTTAAGGTGTGCAGTGTTTTTCCTGTGTATTAAACCTTTCCATTTTACGTTTTAGAAAATTTTATGTA
  TTTTAAAATAAGGGGAAGAGTCATTTTCACTTTTAAACTACTATTTTTCTTTCCAAGTCATTTTTGTTTT
  TGGTTTCTTATTCAAAGATGATAATTTAGTGGATTAACCAGTCCAGACGCACTGATCTTTGCAAAGGAGA
  CTTAATTTCAAATCTGTAATTACCATACATAAACTGTCTCATTATACGTATGCATTTTTTTAGTTTGTTT
  TTGTTTGGTATAAATTAATTTGTTAATTAAATATTTCTTAAGTATAAACCTTATGAACTACAGTGGAGCT
  ACACTCATTGAAATGTAATTTCAGTTCTAAAAAGATGTAATAATCATTTTAGAATTAAAATTTATTCTAC
  TTTTAAATAAATTATGAATATTAAAGGTGAAAATTGTATAAATTACTTTGATTCCATTTTAAGTGGAGAC
  ATATTTCAGTGATTTTTAGTAACCTTTAAAAATGTATAATGACTTTTAAAATTTGTAGAATTGAAAAGAC
  GCTAATAAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000788.2
• Summary: ' Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008]'
• Locus ID: 1633