DGKI (NM_004717) Human 3' UTR Clone

CAT#: SC215442

3`UTR clone of diacylglycerol kinase iota (DGKI) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DGKI
• Synonyms: DGK-IOTA
• ACCN: NM_004717
• Insert Size: 1598
• Sequence Data:
  Insert Sequence

  >SC215442 3'UTR clone of NM_004717
  The sequence shown below is from the reference sequence of NM_004717. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATGAGGACCTGGAAACTGCTGTTTGACCCTGGTATTCGGGCAAAGAGGACATGAGCAAGCGTATCACATC
  TGCCCTCCCTGCAATTGGGCAGCTCCCCTGGAAGAAGCTGATGGAATTCATATATCTGTCTCTCTCCTGC
  AAGAATCTACCTGAGACCATGCCACTAGCTTTTAAGGGCTACCAAGATGTACAACAGAACATGATAGCCC
  ATTGAGAAGGAGGCAGGATACCTGGAGATTTGTGGAATACAGTACGAGTTCCACAAAATTTGATCCTTAT
  TGCTTCCAGCAAGTAGCATGAACTTCTGTGTTCACCTGTATAATTTATTTTAAAGATTCAAAGGATGTTC
  GTATAAATGGCACTGCTCCATCCTCCCCCTATGCATTGGTTTTTTTCCCTGTACCATACAATTCTACTGT
  AACTACCCATCAACTTAAAGAAAAATATTATCTCTTCTCTTTACATTCAGTCTTGGAAGACCACAAGATT
  GTCTGAAGGCCTTCTAAAACCTTCTGAATGTCCTGCAGAAATATAACTGTAAAACCACTTCCATTTCTAA
  GACTAAATATATCAAGACTATTTAGTGACTCTCTCTGCATGTCCCCCTCACCCGCCAACCCTCCGTTTCA
  TTATATAGGAGCTGGGAAGTGCCACATGGATAATGTCAACTTGTGTGCTATATCTCTGAGGAATGGTGAG
  GTGGCATGGGAGATGTCTGTGCTTGGAGGTACCTCAGAGAGGTAACCCAGGGGTCAGCCCAGGCTGCTGG
  GCTGTAGCCAATAGCCATGCAGGACTGGTTCAGCTTGGGCTGTCTGTACAGCTCCGTACTGCCTATGTGT
  AGCCATCTTTGCCTTTTGCTGCAATAGAAGATGAGCAAAGGATTAAACAGAGGCCCACAGCTAGTTTGCA
  GAACCACTCAATTTTAAGTGCTGTTTAAATTGCAGAGCAAATAATCCTGTGTGGGAACTGTGGTTACAGG
  AAATGGAGCACTCTAACAATGTTTACTTCTAAACTTTGTTGAATGATAATAGAAAGCACCCTAATTGACT
  TGGAAAAAAAAAACAGCAAAAGCAAAAGTAGCAACATATGTCAACATATGTCACTGAAATAGGAAACAGT
  CATTGGAATGTTGCACAGAGGCTAATAACTATAGACTGTTGGATACAGTGGTGAGAGGAGCCCCATTTTA
  GGTCTTTCTTTTAGGTTTTTGGTTTTCATTACTCCAAGTAATCCTTGACCCAAGAACAAAGGCTTGTTGT
  ATGAGTTCCACTGCCAGATTTATGGGATGCCTGGATCATTCAGAAGGATGCTTCAACTATTATTTGTCAG
  GTCCAAAGGTCGTACTTAATAACCCCATTTTCTATGTATGGGGTAGTCTAATATATTATTTTATCTACTT
  TATTTTTCCCTTTTCAGAAAGTCCTTAGTGCAAACCACCATTGGAATCTAGCCAGAAATGTCTGTCAGAT
  AGTTAGAATTGTAACATCTAAACCTGCCACGGATCGAATGGTACTTACAGGTACCTCTCTTAGGGACTCT
  GTGATCCCTAAAATATCAGAAGAAAATGTCTGTCTTTCTGTCCAAATATCTACTTGAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_004717.2
• Summary: This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008]
• Locus ID: 9162