FPGT (NM_003838) Human 3' UTR Clone

CAT#: SC215556

3`UTR clone of fucose-1-phosphate guanylyltransferase (FPGT) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: FPGT
• Synonyms: GFPP
• ACCN: NM_003838
• Insert Size: 1624
• Sequence Data:
  Insert Sequence

  >SC215556 3'UTR clone of NM_003838
  The sequence shown below is from the reference sequence of NM_003838. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TTAAAAAGCAGTTTGATGTAGAGATATTTTAAATATTGTACACTTTGCCTTTTTGAGTAACATTCCAGAG
  ATAGGTATTTTTTGTAGGCTGTTTCACTGAACTCAGTTAATGAAAACTGTATTAACATAATTGTTGTAGC
  ATAATATTAATAGTGCAAAAGTACATATAAGTCATTTTGATGAAAAATATTCCAAGACTAAGTTGAGAAA
  AGAGATACTATTTTGGATGTGTATCAGTATTTTTGTTTTTAATAATGATTGATTTGTGGAGCATTGTTTT
  TTCACATAATTAGTTTTAAAGGTAATTTTCTAAGCATACCTTTGGAATTTTTCCATCTTTTTTGAGGCTT
  TTGGTCCAGTGAAGTTCTAAGTATTCACTGGCACTTCTCTCCTCAACTGTAATTCTATTTTTAATAATAA
  AAATGGCATACTGTAGGGTCTTCAGAGTAGTGTAGGAATACTGTAGAAATACTTTTTCAGAAACGAATCC
  ATAGCTGACAAATTCACTCAGTGCCCAATATATTGTGATTATTTTCGTTGATAAAGAACTAGATACAAAG
  ACCTCTGAAATTGATGATAAAATTTGTATCTCATTAATTTTATCAAAATGAAACTAAAAGTACATATGTA
  TTATACACTTGAACATGTGTTTGTATATCTTTAAAATTTTCCTTTATGTTCCATTCCATAGGAAAACACA
  CATATGCACACAAAACTCAGTTATCTGTGGGGAAAGTGGTAGTAATAATTGAGATTCATCAATAATCATA
  TAATTTCACTATAGACATTACTTGCATTATCTCCTATCAGTCCTTCTAACAAAACTTCAATTACCAGATG
  AACTGACTTTAGTTTTCATCTTATTTTTTGTCCACATGCTGGTGATGCTGAGAAACAATAATTGGCCCAA
  ATGCAGACATCAAGAAGCAGGCAGGAAAATGGAAAAACTGAGATAATACACAGGTGATAAACAGTTTCGA
  GAAAATAAAAATTGGCTAGAATCTCAAGTTATGTGTTTCTATATTGGTATAAGCATATAAGTGAAAAGTC
  TTATAAGTGTAATAGAGAAAAGATTTGTCAGTGTGTTTTTTTAAATGAAATAACTAGTCTGTGCTACTTT
  ATGTCAATATAAAAATTGGTAAACTAGAAGTAACTTGTCCACAACCCTCAGTTATGATACTTATGTGCGT
  GTTTTTTTTTCCAAAGTTTTTCCCAAGGAGAAGATACAAATATATGGTAGCTATTGTTTAAAAATGATTG
  ATTTACTTGCAGATTTTTCAGAGGATGTTATGTCTTTGTCATTCATTAAATGTTCAAAATTGTAGTTTTA
  CCAAATCTGAAGTGCCATCAGTTATAAGAATCACCATTATTTTATGTTCCATTAAGAAAAACACAATAAA
  ACATGACACTGCTTTTTTTGTTACTTGAAATTTTCATATTTATTGGAGAGCTCTTTTAGATGTTTTTAAA
  TACAGATTTTTATTTAACCCTCTTGCATATACATATAAAATAACAAAAAAATAAAATATTGAGATATCAT
  CAAGAAATTTCTACAGCTTCACATTCAAACTCTTCAGCACTTTCAGAGCTATTGATATGCATATGTCCAC
  CTAGTACCATCTTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_003838.2
• Summary: L-fucose is a key sugar in glycoproteins and other complex carbohydrates since it may be involved in many of the functional roles of these macromolecules, such as in cell-cell recognition. The fucosyl donor for these fucosylated oligosaccharides is GDP-beta-L-fucose. There are two alternate pathways for the biosynthesis of GDP-fucose; the major pathway converts GDP-alpha-D-mannose to GDP-beta-L-fucose. The protein encoded by this gene participates in an alternate pathway that is present in certain mammalian tissues, such as liver and kidney, and appears to function as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids. This pathway involves the phosphorylation of L-fucose to form beta-L-fucose-1-phosphate, and then condensation of the beta-L-fucose-1-phosphate with GTP by fucose-1-phosphate guanylyltransferase to form GDP-beta-L-fucose. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream TNNI3 interacting kinase (TNNI3K) gene. [provided by RefSeq, Dec 2010]
• Locus ID: 8790