SRD5A2 (NM_000348) Human 3' UTR Clone

CAT#: SC215601

3`UTR clone of steroid-5-alpha-reductase alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SRD5A2
• ACCN: NM_000348
• Insert Size: 1638 bp
• Sequence Data:
  Insert Sequence

  >SC215601 3'UTR clone of NM_000348
  The sequence shown below is from the reference sequence of NM_000348. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCAAATCTCGGAAAGCCCTTATTCCATTCATCTTTTAAAGGAACCAAATTAAAAAGGAGCAGAGCTCCCA
  CAATGCTGATGAAAACTGTCAAGCTGCTGAAACTGTAATTTTCATGATATAATAGTCCCGTATATATGTA
  ATAGTAGGTCTCCTGGCGTTCTGCCAGCTGGCCTGGGGATTCTGAGTGGTGTCTGCTTAGAGTTTACTCC
  TACCCTTCCAGGGACCCCTATCCTGATCCCCAACTGAAGCTTCAAAAAGCCACTTTTCCAAATGGCGACA
  GTTGCTTCTTAGCTATTGCTCTGAGAAAGTACAAACTTCTCCTATGTCTTTCACCGGGCAATCCAAGTAC
  ATGTGGCTTCATACCCACTCCCTGTCAATGCAGGACAACTCTGTAATCAAGAATTTTTTGACTTGAAGGC
  AGTACTTATAGACCTTATTAAAGGTATGCATTTTATACATGTAACAGAGTAGCAGAAATTTAAACTCTGA
  AGCCACAAAGACCCAGAGCAAACCCACTCCCAAATGAAAACCCCAGTCATGGCTTCCTTTTTCTTGGTTA
  ATTAGGAAAGATGAGAAATTATTAGGTAGACCTTGAATACAGGAGCCCTCTCCTCATAGTGCTGAAAAGA
  TACTGATGCATTGACCTCATTTCAAATTTGTGCAGTGTCTTAGTTGATGAGTGCCTCTGTTTTCCAGAAG
  ATTTCACAATCCCCGGAAAACTGGTATGGCTATTCTTGAAGGCCAGGTTTTAATAACCACAAACAAAAAG
  GCATGAACCTGGGTGGCTTATGAGAGAGTAGAGAACAACATGACCCTGGATGGCTACTAAGAGGATAGAG
  AACAGTTTTACAATAGACATTGCAAACTCTCATGTTTTTGGAAACTAGTGGCAATATCCAAATAATGAGT
  AGTGTAAAACAAAGAGAATTAATGATGAGGTTACATGCTGCTTGCCTCCACCAGATGTCCACAACAATAT
  GAAGTACAGCAGAAGCCCCAAGCAACTTTCCTTTCCTGGAGCTTCTTCCTTGTAGTTCTCAGGACCTGTT
  CAAGAAGGTGTCTCCTAGGGGCAGCCTGAATGCCTCCCTCAAAGGACCTGCAGGCAGAGACTGAAAATTG
  CAGACAGAGGGGCACGTCTGGGCAGAAAACCTGTTTTGTTTGGCTCAGACATATAGTTTTTTTTTTTTTT
  ACAAAGTTTCAAAAACTTAAAAATCAGGAGATTCCTTCATAAAACTCTAGCATTCTAGTTTCATTTAAAA
  AGTTGGAGGATCTGAACATACAGAGCCCACATTTCCACACCAGAACTGGAACTACGTAGCTAGTAAGCAT
  TTGAGTTTGCAAACTCTTGTGAAGGGGTCACCCCAGCATGAGTGCTGAGATATGGACTCTCTAAGGAAGG
  GGCCGAACGCTTGTAATTGGAATACATGGAAATATTTGTCTTCTCAGGCCTATGTTTGCGGAATGCATTG
  TCAATATTTAGCAAACTGTTTTGACAAATGAGCACCAGTGGTACTAAGCACAGAAACTCACTATATAAGT
  CACATAGGAAACTTGAAAGGTCTGAGGATGATGTAGATTACTGAAAAATGCAAATTGCAATCATATAAAT
  AAGTGTTTTTGTTGTTCATTAAATACCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000348.3
• Summary: 'This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008]'
• Locus ID: 6716