PDE7A (NM_002603) Human 3' UTR Clone

CAT#: SC215670

3`UTR clone of phosphodiesterase 7A (PDE7A) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PDE7A
• Synonyms: HCP1; PDE7
• ACCN: NM_002603
• Insert Size: 1624 bp
• Sequence Data:
  Insert Sequence

  >SC215670 3'UTR clone of NM_002603
  The sequence shown below is from the reference sequence of NM_002603. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCTCAGGAAAATCGGTTATCATAACCCCCAGAACCAGTGGGACAAACTGCCTCCTGGAGGTTTTTAGAAA
  TGTGAAATGGGGTCTTGAGGTGAGAGAACTTAACTCTTGACTGCCAAGGTTTCCAAGTGAGTGATGCCAG
  CCAGCATTATTTATTTCCAAGATTTCCTCTGTTGGATCATTTGAACCCACTTGTTAATTGCAAGACCCGA
  ACATACAGCAATATGAATTTGGCTTTCATGTGAAACCTTGAATATGCAAAGCCCAGCAGGAGAGAATCCG
  AAAGGAGTAACAAAGGAAGTTTTGATATGTGCCACGACTTTTTCAAAGCATCTAATCTTCAAAACGTGAA
  ACTTGAATTGTTCAGCAACAATCTCTTGGAATTTAACCAGTCTGATGCAACAATGTGTATCTTGTACCTT
  CCACTAAGTTCTCTCTGAGAAAATGGAAATGTGAAGTGCCCAGCCTCTGCTGCCTCTGGCAAGACAATGT
  TTACAAATCAACTCTGAAAATATTGGTTCTAAATTGCCTTGGAGCATGATTGTGAAGGAACCACTCAAAC
  AAATTTAAAGATCAAACTTTAGACTGCAGCTCTTTCCCCCTGGTTTGCCTTTTTCTTCTTTGGATGCCAC
  CAAAGCCTCCCATTTGCTATAGTTTTATTTCATGCACTGGAAACTGAGCATTTATCGTAGAGTACCGCCA
  AGCTTTCACTCCAGTGCCGTTTGGCAATGCAATTTTTTTTAGCAATTAGTTTTTAATTTGGGGTGGGAGG
  GGAAGAACACCAATGTCCTAGCTGTATTATGATTCTGCAGTGAAGACATTGCATGTTGTTTTCACTACTG
  TACACTTGACCTGCACATGCGAGAAAAAGGTGGAATGTTTAAAACACCATAATCAGCTCAGGGTATTTGC
  CAATCTGAAATAAAAGTGGGATGGGAGAGTGTGTCCTTCAGATCAAGGGTACTAAAGTCCCTTTCGCTGC
  AGTGAGTGAGAGGTATGTTGTGTGTGAATGTACGGATGTGTGTTTGCGTGCATGTTTGTGCATGTGTGAC
  TGTGCATGTTATGTTTCTCCATGTGGGCAAAGATTTGAAATGTAAGCTTTTATTTATTATTTTAGAATGT
  GACATAATGAGCAGCCACACTCGGGGGAGGGGAAGGTTGGTAGGTAAGCTGTAACAGATTGCTCCAGTTG
  CCTTAAACTATGCACATAGCTAAGTGACCAAACTTCTTGTTTTGATTTGAAAAAAGTGCATTGTTTTCTT
  GTCCCTCCCTTTGATGAAACGTTACCCTTTGACGGGCCTTTTGATGTGAACAGATGTTTTCTAGGACAAA
  CTATAAGGACTAATTTTAAACTTCAAACATTCCACTTTTGTAATTTGTTTTAAATTGTTTTATGTATAGT
  AAGCACAACTGTAATCTAGTTTTAAGAGAAACCGGTGCTTTCTTTTAGTTCATTTGTATTTCCCTTGTTA
  CTGTAAAAGACTGTTTATTAATTGTTTACAGTTTGTTGCAACAGCCATTTTCTTGGGAGAAAGCTTGAGT
  GTAAAGCCATTTGTAAAAGGCTTTGCCATACTCATTTTAATATGTGCCTGTTGCTGTTAACTTTTGATGA
  ATAAAAACCTATCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002603.2
• Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE7 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]'
• Locus ID: 5150