OXCT1 (NM_000436) Human 3' UTR Clone

CAT#: SC215951

3`UTR clone of 3-oxoacid CoA transferase 1 (OXCT1) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: OXCT1
• Synonyms: OXCT; SCOT
• ACCN: NM_000436
• Insert Size: 1675 bp
• Sequence Data:
  Insert Sequence

  >SC215951 3'UTR clone of NM_000436
  The sequence shown below is from the reference sequence of NM_000436. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACTCATGCCAATGCAGCAGATCGCAAATTGAAATATGGATATTTGTACCAGGCTGCGTGTTTTTCATTTT
  AAACACACAAGATTTAATTGAAAGGACATCAATAATCATAATTGTGTATTTAACAGGTGGTTTTTTATTA
  GTTTTCTTGTGTTTCAGACTTTATGCAGCCATATAAACTGTTCTCTAGGCATGCTGTGACATTTTAATAA
  AAAGCAAAAGGAGCATTTATAATTATCTCATTTGTTAAGGCTGAGAAGGTTGTTTTTATAATAGGTAATT
  ATATTGAATGCATTTTCACTGAATATGGTATGTATGCTAAATTATATGAACCTTTCCCCAAGAAGGGCCC
  TAGAAATTGATGTGGCTTTCCTCTTAAATATTAATTATTAGTCCTGAAAGAAAGATAACATATGTGATTT
  TTGTGGTTAGGAGAGTTGCTGTCATGATTGTTTTTTCTTCAGCCTCCTCTGACTTTTCTTTTGGGGCTTC
  AGATTTTATGATTACATCTTGTCCCCCTAGAACATCCCCCTTCCTCCCATACTGCTTTTAAACAGATGCC
  CAAGAAGGCAAGCAGGAATGCCTCTTGTGGGGGAGGGCAGGGAGAAATAACTAGTTCAAACCAACTATCT
  ATCTATGCTTTGCAAAGACTAAGGCGTATTATAGGAAGAGGGCTAGAAACCTAACTGATTCTTCTCAGTT
  TTCTCATTTTAAAACAGCCCAGTATTCCTTTGTATCCTCAAGGGTCCTTGAGAATACTTCTGTTATTGAG
  ACCCTGTGGGCTACTTGTACTGTACCTCCTCTCAAGCCAAGAAGGGCTGTGGGATAATTTACCATGAATC
  CTTAGTAGCAATGACAGCAGAGTTAAAAAATAAAAGGTGTTTTACTTTCAGGCTCTTGTTTTGGTTCAGA
  GGAGATTTTAAATATTGAATGACACTTCTACAGAACAACGGTTTTTCTTCTGCCAAGGCTACTTCCTTTA
  ACGAAGTGCCTTTAATTCAGCCTTATCCAACTAGGGAAAATAATGTTGGACAAGTCTAGGATTTGAAGAG
  TCAGTGAACTTTTAGTGTCAGGGAATAAACATGGTGGGTAGATTAGGTTTGAAAAAAACTTCCTTAGAGG
  TATTTATTCTCAATACCTGACAGGGGCCCATGGGAATGACTTCAGAAGCATCCCGGATAATAGATGGGTA
  AAAAGTCTAGGCACCCTGAAGAACAGGTGAGACAGCTGGCCTCTGGACAGAGGTAGGCATAGTACAGTAC
  GATATATCATTCCTCTGGTCCTAAATATACAAACTTATTCATGTTTTTAGGTGATGATGGTCATTGAAAC
  TCACTTCTTTTCAGGTGTAGCTACAATTGTGTAATGTACAATATTAGAGAAAGGACAGGCTTTTTATGAG
  TAACACACACCATATATAAAACAGCCTTTCTGGCTGACCACATGGTTAAATGCATACCTTCCCAGTACTG
  GGGGGAAAAAATGACCCTTCTTAGAATGTGCAAGTTCCATGAGAGTAATATATTGATATGATTTTGAAAA
  GAATTGTTGATAGTTACATCTTCAAACTTATCATTCCAGTATGCATCTTTAAGATAATGTGATTCTAAGT
  AGATGACTTTATATTCTTGATTAAAGAGTGCTATACATGTTAAGAAATGCATTAAGGAATACAAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000436.3
• Summary: 'This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. [provided by RefSeq, Jul 2008]'
• Locus ID: 5019