Factor VII (F7) (NM_000131) Human 3' UTR Clone

CAT#: SC216011

3`UTR clone of coagulation factor VII (serum prothrombin conversion accelerator) (F7) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: F7
• Synonyms: SPCA
• ACCN: NM_000131
• Insert Size: 1683 bp
• Sequence Data:
  Insert Sequence

  >SC216011 3'UTR clone of NM_000131
  The sequence shown below is from the reference sequence of NM_000131. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGAGTCCTCCTGCGAGCCCCATTTCCCTAGCCCAGCAGCCCTGGCCTGTGGAGAGAAAGCCAAGGCTGC
  GTCGAACTGTCCTGGCACCAAATCCCATATATTCTTCTGCAGTTAATGGGGTAGAGGAGGGCATGGGAGG
  GAGGGAGAGGTGGGGAGGGAGACAGAGACAGAAACAGAGAGAGACAGAGACAGAGAGAGACTGAGGGAGA
  GACTCTGAGGACATGGAGAGAGACTCAAAGAGACTCCAAGATTCAAAGAGACTAATAGAGACACAGAGAT
  GGAATAGAAAAGATGAGAGGCAGAGGCAGACAGGCGCTGGACAGAGGGGCAGGGGAGTGCCAAGGTTGTC
  CTGGAGGCAGACAGCCCAGCTGAGCCTCCTTACCTCCCTTCAGCCAAGCCCACCTGCACGTGATCTGCTG
  GCCTCAGGCTGCTGCTCTGCCTTCATTGCTGGAGACAGTAGAGGCATGAACACACATGGATGCACACACA
  CACACGCCAATGCACACACACAGAGATATGCACACACACGGATGCACACACAGATGGTCACACAGAGATA
  CGCAAACACACCGATGCACACGCACATAGAGATATGCACACACAGATGCACACACAGATATACACATGGA
  TGCACGCACATGCCAATGCACGCACACATCAGTGCACACGGATGCACAGAGATATGCACACACCGATGTG
  CGCACACACAGATATGCACACACATGGATGAGCACACACACACCAATGCGCACACACACCGATGTACACA
  CACAGATGCACACACAGATGCACACACACCGATGCTGACTCCATGTGTGCTGTCCTCTGAAGGCGGTTGT
  TTAGCTCTCACTTTTCTGGTTCTTATCCATTATCATCTTCACTTCAGACAATTCAGAAGCATCACCATGC
  ATGGTGGCGAATGCCCCCAAACTCTCCCCCAAATGTATTTCTCCCTTCGCTGGGTGCCGGGCTGCACAGA
  CTATTCCCCACCTGCTTCCCAGCTTCACAATAAACGGCTGCGTCTCCTCCGCACACCTGTGGTGCCTGCC
  ACCCACTGGGTTGCCCATGATTCATTTTTGGAGCCCCCGGTGCTCATCCTCTGAGATGCTCTTTTCTTTC
  ACAATTTTCAACATCACTGAAATGAACCCTCACATGGAAGCTATTTTTTAAAAACAAAAGCTGTTTGATA
  GATGTTTGAGGCTGTAGCTCCCAGGATCCTGTGGAATTGGATGTTCTCTCCCTGCCACAGCCCTTGTCAA
  TGATATTTCACAGAGACCCTGGGAGCACCTGCTCAAGAGTCAGGGACACACGCATCACTAAATGCAAGTT
  CCCAGGCCCTGGCTGCAGTGGGAGGACCTGGCAAGCTGCACTCTTGCTGAGTCCCCAGGGTGGTGGAAGA
  AGAATGAGAAACACATGAACAGAGAAATGGGGAGGTGACAAACAGTGCCCCCACTCAGACTCCGGCAAGC
  ACGGCTCAGAGAGTGGACTCGATGCCATCCCTGCAGGGCCGTCCTGGGCACCACTGGCACTCACAGCAGC
  AAGGTGGGCACCATTGGCACTCACAGCAGCAAGGCAGGCACCAGCAACCCACCTCGGGGGCACTCAGGCA
  TCATCTACTTCAGAGCAGACAGGGTCTATGAACTACAGCCGTGGGCTGCTTCCAAGGCACCCTGCTCTTG
  TAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000131.3
• Summary: 'This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]'
• Locus ID: 2155