PDE7A (NM_002604) Human 3' UTR Clone

CAT#: SC216132

3`UTR clone of phosphodiesterase 7A (PDE7A) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PDE7A
• Synonyms: HCP1; PDE7
• ACCN: NM_002604
• Insert Size: 1714 bp
• Sequence Data:
  Insert Sequence

  >SC216132 3'UTR clone of NM_002604
  The sequence shown below is from the reference sequence of NM_002604. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CATCCAGATTGGTAACTATACATATTTAGATATAGCTGGTTAGAAAAATGCCACTGTTTTTATCAAGAAG
  GGAAATATATTTGAAATATAAAATATTAAAATTATGCTCATTTCTATTTTTAAAAATAATTTAAGAAATT
  TTACCCTTGTTTTCCCTTGTTATGGCTCTTCTAATTCTCATTTAATTTTAGGATGTAAAAAGTATATTTT
  TGCAGAACAGGCAGCAGCAATAACTTGTTTCTGTTCTTATGTAAATAAGAATCCATTATTCGCTCATGTG
  GAAGCTTCTTTTGCATCATTTGGGACTGCCATTTAAAAAAGGATAGGTAAACAAAGAAATGACAAAAATA
  AAATAAATAAAATAAAAATGGATAGGTGGTGACCCACTGAGCCTGATCATAATACGAAGACCAGCTTCTG
  CCACTGCCTTTCCAGACTCTTACCACTGCCTGTTGATTAAATCTAACTCTTCAACATCCTAGACAGGCCC
  TTATAATCTTGCTTCAAATGCTGTGCAGCCATCTTGCCTCAACTTCCCTCTCATTTGCCTACAGCATCTC
  GGGACGCTTCTGTGTTTCCCAAGTATACGCTGTTCTTTCGCTCTTTGTGCCTCGCCAGTGCTTTCCATGT
  GCCTCGTAGAGTTATTTTTCTTGAAGAGGCAGCTCAAATGTCACCTTCTCCAGAAGCTGCTCTCCACTTG
  CTTTAGGCAGAGTCAGTCACTTTTCTTCTAGATTCCAAAGTGCCTGATCCACTTGGTTGTGGATTCCTGG
  AGCCTAGCACCACACCAGAAGCACGAGGCCCTTGAGAACTGTGTGTTGAGTGAACTAATAACTGTATTAT
  AGAAAGCATAATGAAAATGTCCTGTGACTGAAGTATGTGTAGCTTGTTGCAGGAGTTCACAGGAAAGTTG
  ACTAGGATTGAGTGTGTTGGGCTTTGGGTATAAAGGAGGGGGATTCTACGGGGGCAGTAGCTCAACAAGG
  AATAGAGGGAGGAGTGTAATTTTGGTAGCTGGTGTTGAATAGGGCCTTTGAGAATCAGACTGAACACAGT
  GAAATATGTGCCCAAAGTTCAGAAAGATGAAGTTTCCAGAAACTAAGAAGGTAGCACAATATGTGGCATC
  ATACTCAGAAAGGAAGACCATGCCATGGGGCCAGAAATTCAGAAACGTAATTCTTACATTGTGATTGCAA
  TGGATACTCATGGAAGAAAGTGGGTAGTGGCCGATTTGACTTCAGAGTGACAGGTAGAGAAGGGAAGAGC
  GTGTAGAACTGTGGCCAGACTTTAGGAGTGTGAGGGATGCTGAATCTCCCAGAGAGCTCACACTGGCCAG
  GAATGCTGAGAGTAGCAGATGCTTTTCTTTTGGGAGGATAGTAAAACAATTTAGAACCAGATATGCTTTG
  TCTTGATTCTCAAGTAGAATAATCTTCAAATGCAAAAGAATACATTAGAAATGGACAAAAGTGGCCAGGA
  GCGGTAGCTCATACTTGTAACCCAGCACTTTGGGAAGCCGAGGCGGGCTGATCGCTTGAGGTCAGGAGTT
  CGAGACCAGCCTGGCCAAAATAGTGAAACTCACGTTTCTACTAAAAATACAAAAATTAGCTGGGTGTGAT
  GGCCACTTGGGAGGCTGAGATAGGAGAATCGCTTGAACCTGGGAGGCAGAGGTTGCAGTGAGCCAATATC
  GTGCCACTGCATTCCAGCCTGGGTGACAGAATGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002604.2
• Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE7 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]'
• Locus ID: 5150