LAMC2 (NM_005562) Human 3' UTR Clone

CAT#: SC216193

3`UTR clone of laminin gamma 2 (LAMC2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: LAMC2
• Synonyms: B2T; BM600; CSF; EBR2; EBR2A; LAMB2T; LAMNB2
• ACCN: NM_005562
• Insert Size: 1733 bp
• Sequence Data:
  Insert Sequence

  >SC216193 3'UTR clone of NM_005562
  The sequence shown below is from the reference sequence of NM_005562. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGCTCTTGAGCAACAGTGAAGCTGCCATAAATATTTCTCAACTGAGGTTCTTGGGATACAGATCTCAGG
  GCTCGGGAGCCATGTCATGTGAGTGGGTGGGATGGGGACATTTGAACATGTTTAATGGGTATGCTCAGGT
  CAACTGACCTGACCCCATTCCTGATCCCATGGCCAGGTGGTTGTCTTATTGCACCATACTCCTTGCTTCC
  TGATGCTGGGCAATGAGGCAGATAGCACTGGGTGTGAGAATGATCAAGGATCTGGACCCCAAAGAATAGA
  CTGGATGGAAAGACAAACTGCACAGGCAGATGTTTGCCTCATAATAGTCGTAAGTGGAGTCCTGGAATTT
  GGACAAGTGCTGTTGGGATATAGTCAACTTATTCTTTGAGTAATGTGACTAAAGGAAAAAACTTTGACTT
  TGCCCAGGCATGAAATTCTTCCTAATGTCAGAACAGAGTGCAACCCAGTCACACTGTGGCCAGTAAAATA
  CTATTGCCTCATATTGTCCTCTGCAAGCTTCTTGCTGATCAGAGTTCCTCCTACTTACAACCCAGGGTGT
  GAACATGTTCTCCATTTTCAAGCTGGAAGAAGTGAGCAGTGTTGGAGTGAGGACCTGTAAGGCAGGCCCA
  TTCAGAGCTATGGTGCTTGCTGGTGCCTGCCACCTTCAAGTTCTGGACCTGGGCATGACATCCTTTCTTT
  TAATGATGCCATGGCAACTTAGAGATTGCATTTTTATTAAAGCATTTCCTACCAGCAAAGCAAATGTTGG
  GAAAGTATTTACTTTTTCGGTTTCAAAGTGATAGAAAAGTGTGGCTTGGGCATTGAAAGAGGTAAAATTC
  TCTAGATTTATTAGTCCTAATTCAATCCTACTTTTCGAACACCAAAAATGATGCGCATCAATGTATTTTA
  TCTTATTTTCTCAATCTCCTCTCTCTTTCCTCCACCCATAATAAGAGAATGTTCCTACTCACACTTCAGC
  TGGGTCACATCCATCCCTCCATTCATCCTTCCATCCATCTTTCCATCCATTACCTCCATCCATCCTTCCA
  ACATATATTTATTGAGTACCTACTGTGTGCCAGGGGCTGGTGGGACAGTGGTGACATAGTCTCTGCCCTC
  ATAGAGTTGATTGTCTAGTGAGGAAGACAAGCATTTTTAAAAAATAAATTTAAACTTACAAACTTTGTTT
  GTCACAAGTGGTGTTTATTGCAATAACCGCTTGGTTTGCAACCTCTTTGCTCAACAGAACATATGTTGCA
  AGACCCTCCCATGGGGGCACTTGAGTTTTGGCAAGGCTGACAGAGCTCTGGGTTGTGCACATTTCTTTGC
  ATTCCAGCTGTCACTCTGTGCCTTTCTACAACTGATTGCAACAGACTGTTGAGTTATGATAACACCAGTG
  GGAATTGCTGGAGGAACCAGAGGCACTTCCACCTTGGCTGGGAAGACTATGGTGCTGCCTTGCTTCTGTA
  TTTCCTTGGATTTTCCTGAAAGTGTTTTTAAATAAAGAACAATTGTTAGATGCCTGGGGTCAGTTTCTGA
  TTTACTAAACTCTAAGCCTATGATGGTACCAAAGTAATGGAAATGAAAATGCCACAGAAATGGCAATAGG
  TCCTAGAGTTAGCATCTTCAGTTCCCACTTCTGAGCATCTTACTTCGCCCATTCCAACCATACAGGATTG
  ATGATGCTGTCAATGAGATTTTTATGAGGTTTAACTGATGCTGTCTTCTAAGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005562.2
• Summary: 'Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two tr'
• Locus ID: 3918