CHMP2B (NM_014043) Human 3' UTR Clone

CAT#: SC216252

3`UTR clone of chromatin modifying protein 2B (CHMP2B) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHMP2B
• Synonyms: ALS17; CHMP2.5; DMT1; VPS2-2; VPS2B
• ACCN: NM_014043
• Insert Size: 1739
• Sequence Data:
  Insert Sequence

  >SC216252 3'UTR clone of NM_014043
  The sequence shown below is from the reference sequence of NM_014043. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGAACGGCAACTCAAGGCTTTAGGAGTAGATTAGTCAAAAGAAGTCATACTATTTTGCTTACTTATAATT
  ATGTAGTATAAACCAAGCACAGTGCAGATTTCTTTTACAAAACACATGTATTTTGCAAAAAAAAAAAAAA
  TGAAGACCATGAGTGAACAGTTGTTTCCTAACCCATGGCTATTTAGAATCTTTTGCCAAAGAATGACAAT
  GATGCAAAAATGGGAACAGTTTGGATTTTAATTAGAACTGTTTAGGAGTGATGATGTGTAAAAAGTTGAC
  TTCTCTTTTGCATGGCACAGAGAAATTATATTCCTTACTTCATGTCAGTTTATGTTCTAAATCTTTTTCA
  CTGAATATAAAAATCTTGTTAAATGCCATTAGGCACCAACTTAAAGAGGGTTGTAAAAATATTAAAAGTA
  TATCGTTAATTCTGTATCTGTTGCTTGTCTTTTGTAAGTGATTATGTGTTATGACCATAGGTGGTTACAG
  CTGCCAAATTATTTTTAAATGGTCAAAAAGAAGAGTGCTATTTAAACATCTGTCTTAAACAAAAACTGTC
  ATAACTTTTCTTTTTTCTTTTTCCATTAGGAGAACATTCTAGTTGGTAAATTTCAAAATGTGCTTGACAC
  CTGCCTTAAATAGCACAGACCTATTGTGCACATCTTTAAATTATTTCAGCTGGCAGAAAAGAATTACATT
  TAAAACTGAAATCAAGGCCTCAATACAAAGATTATCCTGGCTCTTTTCTATCTCTGTGGGCCTAATTGAA
  ATATGTACTCTTATTTTAGACACGCCTCTGTTAAAACAGACCAGGTTTTCCTGGTCTCAGACCTATGATG
  ACTTGTCCCTTTGATGTCACTACTGTGAATTGAATATAATTAGTAAAAATAGACGATGAATAAATAACAC
  TTTATAGTAAGAAAACAATATATTTTGGCCATCTAAAAATGAGAATTATAATTATATGAATTATAATTTA
  AACTGTTTAATTTTGTTTAATGTGTATATTGAATCTTCCAAATTGAAGCCATTATTCTCAATTAAGTACT
  ACAACTATGACAATGCTTGACCTACATTTCTAAAATAAAAATTCACATTTTTTGATAAATAAACTACAGT
  TTTACCAGAAATTACTATCTAAATGTGTATTAGCAGTATTTTTTAAGGTGAAATTGCCTTGGTATCTAAT
  GAATGTGTAGACAGGGAGATAAAATGAAGGATTGCCAGACTAGTTAGAATAGAATTTAGGATTAGGTTAG
  TTTTGAAAAATGATGTTGTAATATATGGGTTCTAACACATCCTACCATAAAAACTGGAGGAGATATGTGT
  AACCTGGTTAATTTGGGATGGTGGACATTTTGGGCTAATACTGACAAAATACATCTTAGGACTAGTATAC
  ATGTGACACGGATTGCTAGGAGGAATGAAAAACTAAACTGTATAGTTTATATTCCGTAAACCATTTTATA
  ATGTTCAAAGATTAGGTTTTGTTATTGATAGTATTAAATACACAGTTTCTCTTAACAGTGATGGGTGAAA
  ACATTTTACCGGATTATGGAATGTTTACCAGAACATGTTTTGATTCTTGAATGTACATAATAATGCCATC
  TAACTTATTTACGTTCTTGTTTACATGTGGGAGCTTTTGTTTTCAAAAATTATTTTGTTAAAAAATCTCA
  ATAAAGATTTATTATTGTTGTTCTTTTCTTACCTTTTTTGCTCTTTTTGGTTCCTGCTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_014043.3
• Summary: This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration. [provided by RefSeq, Jul 2008]
• Locus ID: 25978