UBE2N (NM_003348) Human 3' UTR Clone

CAT#: SC216264

3`UTR clone of ubiquitin-conjugating enzyme E2N (UBC13 homolog yeast) (UBE2N) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UBE2N
• Synonyms: HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN
• ACCN: NM_003348
• Insert Size: 1740 bp
• Sequence Data:
  Insert Sequence

  >SC216264 3'UTR clone of NM_003348
  The sequence shown below is from the reference sequence of NM_003348. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CATGGACTAGGCTATATGCCATGAATAATATTTAAATTGATACGATCATCAAGTGTGCATCACTTCTCCT
  GTTCTGCCAAGACTTCCTCCTCTTTGTTTGCATTTAATGGACACAGTCTTAGAAACATTACAGAATAAAA
  AAGCCCAGACATCTTCAGTCCTTTGGTGATTAAATGCACATTAGCAAATCTATGTCTTGTCCTGATTCAC
  TGTCATAAAGCATGAGCAGAGGCTAGAAGTATCATCTGGATTGTTGTGAAACGTTTAAAAGCAGTGGCCC
  CTCCCTGCTTTTATTCATTTCCCCCATCCTGGTTTAAGTATAAAGCACTGTGAATGAAGGTAGTTGTCAG
  GTTAGCTGCAGGGGTGTGGGTGTTTTTATTTTATTTTATTTTATTTTATTTTTGAGGGGGGAGGTAGTTT
  AATTTTATGGGCTCCTTTCCCCCTTTTTTGGTGATCTAATTGCATTGGTTAAAAGCAGCTAACCAGGTCT
  TTAGAATATGCTCTAGCCAAGTCTAACTTTATTTAGACGCTGTAGATGGACAAGCTTGATTGTTGGAACC
  AAAATGGGAACATTAAACAAACATCACAGCCCTCACTAATAACATTGCTGTCAAGTGTAGATTCCCCCCT
  TCAAAAAAAGCTTGTGACCATTTTGTATGGCTTGTCTGGAAACTTCTGTAAATCTTATGTTTTAGTAAAA
  TATTTTTTGTTATTCTACTTTGCCTTTGTACAGTTTATTTTACTGTGTTTATTTCATTTTCCCAATTTGA
  CAATCGTATTTTAAAATTGAAACTGATGGAACATTCTTTCTTGGTCTTCACCATCTGACAAATTGAATGG
  CAAGAGGTGGATTTTGCCAGTTTCTTTTCACTGATGCAGATTTGTGTTAAGATAGTACTGAATGGAGTAT
  TTATAAACTGGCCCTGAGCATGCATAAAGCATCAGTATCTGACCTTTTTTTAACCTTCTAGGAATTTGAA
  ATAAATGTGTTTGTGTTGTCTGATTAGATGATCATTGGTGTCTTGCCACAATGTTTAAAAATTACTGTAC
  AGGAAAGTCACAGCAAAGATAGCAGTTGTGACTGACATGTAGGACTTTCACAGTTGTGCCACATTTTTGC
  CTAAAATTTGGGTTATGACATTTTTCTTGGTTCTTATCTGAAAATTTCATCTGTAACCTTTCATGTGTGT
  TAAGAAACACTGATCTGATCATTTGGGATTTGCTGAGGCATTTGTGAGTCTTCCTTATAAACCTGATGAG
  CAGATCTCAACTATCTAGCTTGTGTGTCATCAGAAAGGTTTATCCCTTTGAGAGTATCAAGTCCTCAGTT
  AATGATTCTTGCTTTCATCCCTCCAGTATTTGCTGTGGGAGCTCGTTTTATTCTTTAATTTGGAATTCAG
  TAATTTTTCTTCTTTATTGACGAATTCCTCCCCTCACAAAACTGTTCTTTCCCACCTCTCTCCATATCTA
  ATTCCTGATTCTTGTTATTTTTAAGTCATAAATGTAGCCAGTCATAAATACATAAATGTTAACCTTCGGG
  TTGCAACCTTGTCTCTTGCAGTTTAAGGTAATGGATATTGTAGCCCATTTGAATTTTCTTCACTCTTATT
  CTCGTAATTCTGGAGTTTCTTCAGATTGTGGTGTATTTTATTGTGCTCCTATGTAAGATGAAGAATTAAC
  TATTAAAATTACATTTTCAACATACAAAAGCTTTTGATGACTGGTAACTGGTATCCTTCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003348.3
• Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]'
• Locus ID: 7334