TPP1 (NM_000391) Human 3' UTR Clone

CAT#: SC216446

3`UTR clone of tripeptidyl peptidase I (TPP1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TPP1
• Synonyms: CLN2; GIG1; LPIC; SCAR7; TPP-1
• ACCN: NM_000391
• Insert Size: 1805 bp
• Sequence Data:
  Insert Sequence

  >SC216446 3'UTR clone of NM_000391
  The sequence shown below is from the reference sequence of NM_000391. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCTGAAGACTCTACTCAACCCCTGACCCTTTCCTATCAGGAGAGATGGCTTGTCCCCTGCCCTGAAGCTG
  GCAGTTCAGTCCCTTATTCTGCCCTGTTGGAAGCCCTGCTGAACCCTCAACTATTGACTGCTGCAGACAG
  CTTATCTCCCTAACCCTGAAATGCTGTGAGCTTGACTTGACTCCCAACCCTACCATGCTCCATCATACTC
  AGGTCTCCCTACTCCTGCCTTAGATTCCTCAATAAGATGCTGTAACTAGCATTTTTTGAATGCCTCTCCC
  TCCGCATCTCATCTTTCTCTTTTCAATCAGGCTTTTCCAAAGGGTTGTATACAGACTCTGTGCACTATTT
  CACTTGATATTCATTCCCCAATTCACTGCAAGGAGACCTCTACTGTCACCGTTTACTCTTTCCTACCCTG
  ACATCCAGAAACAATGGCCTCCAGTGCATACTTCTCAATCTTTGCTTTATGGCCTTTCCATCATAGTTGC
  CCACTCCCTCTCCTTACTTAGCTTCCAGGTCTTAACTTCTCTGACTACTCTTGTCTTCCTCTCTCATCAA
  TTTCTGCTTCTTCATGGAATGCTGACCTTCATTGCTCCATTTGTAGATTTTTGCTCTTCTCAGTTTACTC
  ATTGTCCCCTGGAACAAATCACTGACATCTACAACCATTACCATCTCACTAAATAAGACTTTCTATCCAA
  TAATGATTGATACCTCAAATGTAAGATGCGTGATACTCAACATTTCATCGTCCACCTTCCCAACCCCAAA
  CAATTCCATCTCGTTTCTTCTTGGTAAATGATGCTATGCTTTTTCCAACCAAGCCAGAAACCTGTGTCAT
  CTTTTCACCCCACCTTCAATCAACAAGTCCTCAATCAACAAGTCCTACTGACTGCACATCTTAAATATAT
  CTTTATCAGTCCACAAGTCCTTCCAATTATATTTCCCAAGTATATCTAGAACTTATCCACTTATATCCCC
  ACTGCTACTACCTTAGTTTAGGGCTATATTCTCTTGAAAAAAAGTGTCCTTACTTCCTGCCAATCCCCAA
  GTCATCTTCCAGAGTAAAATGCAAATCCCATCAGGCCACTTGGATGAAAACCCTTCAAGGATTACTGGAT
  AGAATTCAGGCTTTCCCCTCCAGCCCCCAATCATAGCTCACAAACCTTCCTTGCTATTTGTTCTTAAGTA
  AAAAATCATTTTTCCTCCTCCCTCCCCAAACCCCAAGGAACTCTCACTCTTGCTCAAGCTGTTCCGTCCC
  CTTACCACCCCTGATACAACTGCCAGGTTAATTTCCAGAATTCTTGCAAGACTCAGTTCAGAAGTCACCT
  TCTTTCGTGAATGTTTTGATTCCCTGAGGCTACTTTATTTTGGTATGGCTGAAAAATCCTAGATTTTCTA
  AACAAAACCTGTTTGAATCTTGGTTCTGATATGGACTAGGAGAGAGACTGGGTCAAGTAAGCTTATCTCC
  CTGAGGCTGTTTCCTCGTCTGTTAAGTGTGAATATCAATACCTGCCTTTCATAATCACCAGGGAATAAAG
  TGGAATAATGTTGATAACAGTGCTTGGCACCTGGAAGTAGGTGGCAGATGTTAACGCCCTTCCTCCCTTG
  CACTGCGCCCCCTGTGCCTACCTCTAGCATTGTAACGACCACGTAGTATTGAAATGGCCAGTTTACTTGT
  CTGCCTTCCTTTCCAAGACCGTTGGTGCCTAGAGGACTAGAATCGTGTCCTATTTAACTTTGTGTTCCCA
  GGTCCTAGCTCAGGAGTTGGCAAATAAGAATTAAATGTCTGCTACACCGAAAACC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000391.3
• Summary: 'This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq, Jul 2008]'
• Locus ID: 1200