Thymine DNA glycosylase (TDG) (NM_003211) Human 3' UTR Clone

CAT#: SC216483

3`UTR clone of thymine-DNA glycosylase (TDG) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TDG
• Synonyms: hTDG
• ACCN: NM_003211
• Insert Size: 1785 bp
• Sequence Data:
  Insert Sequence

  >SC216483 3'UTR clone of NM_003211
  The sequence shown below is from the reference sequence of NM_003211. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAGGAAGAAGAAAGCCATGCTTAAGAATGGTGCTTCTCAGCTCTGCTTAAATGCTGCAGTTTTAATGCAG
  TTGTCAACAAGTAGAACCTCAGTTTGCTAACTGAAGTGTTTTATTAGTATTTTACTCTAGTGGTGTAATT
  GTAATGTAGAACAGTTGTGTGGTAGTGTGAACCGTATGAACCTAAGTAGTTTGGAAGAAAAAGTAGGGTT
  TTTGTATACTAGCTTTTGTATTTGAATTAATTATCATTCCAGCTTTTTATATACTATATTTCATTTATGA
  AGAAATTGATTTTCTTTTGGGAGTCACTTTTAATCTGTAATTTTAAAATACAAGTCTGAATATTTATAGT
  TGATTCTTAACTGCATAAACCTAGATATACCATTATCCCTTTTATACCTAAGAAGGGCATGCTAATAATT
  ACCACTGTCAAAGAGGCAAAGGTGTTGATTTTTGTATATGAAGTTAAGCCTCAGTGGAGTCTCATTTGTT
  AGTTTTTAGTGGTAACTAAGGGTAAACTCAGGGTTCCCTGAGCTATATGCACACTCAGACCTCTTTGCTT
  TACCAGTGGTGTTTGTGAGTTGCTCAGTAGTAAAAACTGGCCCTTACCTGACAGAGCCCTGGCTTTGACC
  TGCTCAGCCCTGTGTGTTAATCCTCTAGTAGCCAATTAACTACTCTGGGGTGGCAGGTTCCAGAGAATGC
  AGTAGACCTTTTGCCACTCATCTGTGTTTTACTTGAGACATGTAAATATGATAGGGAAGGAACTGAATTT
  CTCCATTCATATTTATAACCATTCTAGTTTTATCTTCCTTGGCTTTAAGAGTGTGCCATGGAAAGTGATA
  AGAAATGAACTTCTAGGCTAAGCAAAAAGATGCTGGAGATATTTGATACTCTCATTTAAACTGGTGCTTT
  ATGTACATGAGATGTACTAAAATAAGTAATATAGAATTTTTCTTGCTAGGTAAATCCAGTAAGCCAATAA
  TTTTAAAGATTCTTTATCTGCATCATTGCTGTTTGTTACTATAAATTAAATGAACCTCATGGAAAGGTTG
  AGGTGTATACCTTTGTGATTTTCTAATGAGTTTTCCATGGTGCTACAAATAATCCAGACTACCAGGTCTG
  GTAGATATTAAAGCTGGGTACTAAGAAATGTTATTTGCATCCTCTCAGTTACTCCTGAATATTCTGATTT
  CATACGTACCCAGGGAGCATGCTGTTTTGTCAATCAATATAAAATATTTATGAGGTCTCCCCCACCCCCA
  GGAGGTTATATGATTGCTCTTCTCTTTATAATAAGAGAAACAAATTCTTATTGTGAATCTTAACATGCTT
  TTTAGCTGTGGCTATGATGGATTTTATTTTTTCCTAGGTCAAGCTGTGTAAAAGTCATTTATGTTATTTA
  AATGATGTACTGTACTGCTGTTTACATGGACGTTTTGTGCGGGTGCTTTGAAGTGCCTTGCATCAGGGAT
  TAGGAGCAATTAAATTATTTTTTCACGGGACTGTGTAAAGCATGTAACTAGGTATTGCTTTGGTATATAA
  CTATTGTAGCTTTACAAGAGATTGTTTTATTTGAATGGGGAAAATACCCTTTAAATTATGACGGACATCC
  ACTAGAGATGGGTTTGAGGATTTTCCAAGCGTGTAATAATGATGTTTTTCCTAACATGACAGATGAGTAG
  TAAATGTTGATATATCCTATACATGACAGTGTGAGACTTTTTCATTAAATAATATTGAAAGATTTTAAAA
  TTCATTTGAAAGTCTGATGGCTTTTACAATAAAAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003211.4
• Summary: 'The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]'
• Locus ID: 6996