ASAH3L (ACER2) (NM_001010887) Human 3' UTR Clone

CAT#: SC216792

3`UTR clone of alkaline ceramidase 2 (ACER2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ACER2
• Synonyms: ALKCDase2; ASAH3L
• ACCN: NM_001010887
• Insert Size: 1879
• Sequence Data:
  Insert Sequence

  >SC216792 3'UTR clone of NM_001010887
  The sequence shown below is from the reference sequence of NM_001010887. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCTCCTGTGTGCCAACAAGAAATCATCAGTCAAGATCACGTGATGGCAAGATGGTGGCTGGCTTCTCTGC
  TTATCGCCCCTCATGCAGTGGGCTTCCTTTGCTAGGAAGACAGCCAAGGGAGTTCGAATAGTTGGGGTGT
  GGGCTATCTTTTCAAAAATCTATTTGCTGGGGCTCTTAATTTCTTTAGTGTTCTTTGTATGTAGGGATTT
  AAACTTTGTCATATGGTACAAATATTCCCTGCCCCCCTGCAGTTTCCCATTTGTCTTTCAGTATGTTAAT
  ATTTTTGTGCCATACTGGTTTTAAACTTTCATGTTGTCACATCTGTTAATCTTTTCTTTAGGATTTCTGG
  ATTTTGTGTAATTTTTAAAAAGGTCCCCTCCTCCTCCCTAATGTGTCTGTGGACCACCTGGATTCCACTG
  TACAAGGGGAAAAGTGTCTATTCCTTTCCCAAAGATGGAAAATGGAGGGCTTAGGGACACTAGATGCATC
  TTTCTCAGCATCACTTCCAGATGCAGTGACTTGTTGGGCTGCGTCCTTAATGGCCATGGCAGAGCAGTCC
  CTTGGGGGATCCAGCCCTGTACAATGCATCTCTTCCTGGAGAAAGCTGGCCTGCTCCAGACCCCACCATT
  CCCAGGCGCCCTTGGAGTGGACTCTACTGATGACAGACAGACCCTCTGAGAGACAAGACCCTCTGACTCT
  GTGATGGAAGATGCCAGAGATTTTCCTTTGGGGTAATTGTCCTTAAACAAAACCAAACAGATGAAACACA
  CACAGGACTTGTGGCTAAAAAGGCTAGTTTTTCACTTGCATTTCTCAACTAACCCAGGTTTTACATGCAT
  CTGTGAATCCTTTTACTACTACCTCTGTGGAGAGATGGAGAGACTTCAGATAAACGTGAAGCTAATGAGT
  AAAACCCTCTCTGCCAAAACCTACACTCCACTTTAGGCCCTTCTTGAAGATGAGCACAATTTTTAAATAC
  TGAGCACAATTTTTAAATACTGACATCACTTCCTCTTCCCCCTCCCACCCCAGCTCAGCAGCCTCAAATC
  TACAGAGAAGAAGAATTATGGCATGAACATTCCCACAGACCCACCATCTTTAAGACTTGACCTCTGTAAG
  TTTACCAAAGGGCTCCTCACAATTGTGGTGGGGGTTCTGGTTCAAAATTTGGAGCAAACATGAAGTTTTT
  GGAAACGTTTTCTCATTTGAAGCCTCCAGTATGCTGTACTATTCTGGAAATTACCTTCAAGAGTCTCACT
  TCTTGTTTCTGTTGTGTTTTCTGTGGGCATCATGTTCTTCACGCTTGCAGTAGAAGGTGCTTTCTCGGTT
  TCCCAGAGTATCCAACGGCTCACCTTTCTCAAGTGCTGGCAGTAGCTATGCACTCACGGGCTGGTTTGGG
  TCGCTGGTGCAGCAGCGCAAATCTGTTGCCTTCTGAATTTTTCTCACCTAATGTGACACTGGCTACAATG
  AATCTTCTCTTCATCGGGCTGAATGAAAGATTCAAGAACCATCTTCAAGGTGCATGGTGGGAATTATCAA
  CCTCAGGGATACTCATTTTAACTCAGGCGTGTCCTGCTTTGTAACATTCCATTGTTGGGAGAGGGCAGGA
  CAGGTGTGTTCTTCTGTGGGCAGGAGTCATGTCACTGTCCTACATATGTAAGAGTTGGGAAGGTGACGAT
  TTTTGACACATCCAGGAACTCTTACTCTAGTTAGAATTTGTACCAGATCCAAGGTGAAAACCCCAATAAG
  CAACTGAATTTAGAGTTTAAAAATGAATGACTTTATGCTACATCTGTGGTTATCAAATTATATAGGTTGT
  TGAGAAGCAGAACGCTGTTTGTAGTAAGAAATCTTTGTGGAACCCCAGTGTGTGAAGTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001010887.2
• Summary: The sphingolipid metabolite sphingosine-1-phosphate promotes cell proliferation and survival, whereas its precursor, sphingosine, has the opposite effect. The ceramidase ACER2 hydrolyzes very long chain ceramides to generate sphingosine (Xu et al., 2006 [PubMed 16940153]). [supplied by OMIM, Jul 2010]
• Locus ID: 340485