ENAM (NM_031889) Human 3' UTR Clone

CAT#: SC217233

3`UTR clone of enamelin (ENAM) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ENAM
• Synonyms: ADAI; AI1C; AIH2
• ACCN: NM_031889
• Insert Size: 1965
• Sequence Data:
  Insert Sequence

  >SC217233 3'UTR clone of NM_031889
  The sequence shown below is from the reference sequence of NM_031889. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACTGCTTACTACTTCAGGCCTAGGGGTTATCCAACCAAGCATTCTTGGGGAAAGAGAAATCACTGACAT
  TCTATACCAATGGTTCCCAAAATTTTTTCCCCAAGACTTAATTAAGTGTCTGACTGTCTTGGTGATCCTT
  AAGTTTTCTCCCCTCTCAGCAATAGGAGTAGCGACCCAGGTGGAGCTGAGATTAGGCCTCTGGGGATCAC
  CAGATCTAGCACTTTCACAGATTAGTGCAGACCTTAAAAAAGCAAGAAGGCCATGACCATCCCTGCCTCG
  AAACTTGCAAGTCACTTGTCTGAGTTAGTTTCCTTTTGCTTGATGACTTCTTTTGCTCTCAAAGTTCCAT
  ACTTGCAAAATTGGTTTTTCAAGACTGAAAGGCAGATTAACTTTCCATTCTACTTATGGAGATCCACACA
  TCAGTATAGTCCTGATGCACTGTACGTTTTTTGCTTGTGTTGATCTTGTCAGGTTTTTTTCCTACTCAAT
  ATTCTCTTCAAGCATTTTTTTTCTTCCACTGCCACTCTCTTTACCTTTATCATTTCTTTTTCTTAGCTTT
  CTTCCTTCTTTTCTCTCTCCCTCAACCATTCTGTATTTAGCTTTCTAATCCTCATCTTTTTCTCTCTGTA
  TATCACTTCTTACATATTAAATTAGCTATTTTAACCATTTCTAGTCTCTTCTTTATGTCTACCTCCTCCA
  TTATGTCTTCGTCTCTTCCAACCTATCTGTTCTGTCTCCCAATGTACCACTTATCAACTAATCCCTCTCC
  TCTCTGATTCCTTTCTTCCCTTCAATTTATCTTTTTCCCTGTTACACCATTTTCATCTTAAGACTGCATG
  TCACTCTCTTCCTTTTTGTATCCACTCTCCAAATCGTACCCTAAATTTTTCTTGACCCAGCTTAACCATC
  ATTACAGAATATATTTTGGAAATGGTAGACACTGATAGCTACTTTGTTTTTAGTTATCTTAGGGTCTCTA
  CCAAGTAGACACAGTTCAGTTAGAGAGACGTATTTAAGGAGAAGGGAAGGCAGGATACCCAGAACTAGCA
  GCCTAGGGAAAAGGCTTAGGAAGATGGAGAATGAGGAACTGAAGAAACTGCCGCTCTGAGACTGCACATC
  CAGGAAAAAAGACTAGGACTTGCCACACACTGCCAATTCATAATCCGCAGGATCTGTAGAAGACATTTTG
  GGAAACACTGTCTTAAATAAAAACTCAATGTTTAGTTGTTTTTTTTTTTTTTTACTAGTTCAGCATAAGG
  GATTCTACCAGTATGTGCTACTACTAACATTCTTCATGTCCATGCAGAATTACTTACAGACACTCTTTCT
  TGGTATCTTGAATAAATTGTGTAAATTATTTTGAAAAGTGGATATTTCTATATGCCTTGCTTATAATACA
  TCAGGGATTATTATTCTTATTAATAACATGTTGGCCTATTATCTGGATGTCCCTTGCCTAGAGCAGGAAA
  AAAGTCTCAGAATTACCTAAAAACATACTTTATAACTTTAAGAGATTTCTTAGGATACATCTTCAGGGGC
  AGATTTCATAACAACATCTCAAAAATTTGGGGAAACACAACGTTGAAAACTTCCTAAGAAAAGGAATCTT
  GCCCTTGAATCTTCTGAAGTACCTAGCACAGTGTCTTCAACATAGAAGGTACCCAATAAATATATATCAA
  TTCATTTTTTAATGACTTTCAACACTCCCATGAAAACACCGTAGTCTGCTTTTCCTGAAAGGTGACTGTC
  TAGGATGCTGAATGAGCCACCACTCCGTAGCTTTATATATTCAGCAACATTTCCATCATAGAATTTCAGC
  GTTAGAAGGGATCTTAGAGGTAGATTATCCAGGCCATCCCCTACTGCTTACATTCCCCTCCCCCTGGCCA
  GTTTTATATTTTAATCACACATTGAAAAATTCATTAACTTATTCTGTATAAAACTGTCTTTTAAGAATTA
  TCCTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_031889.2
• Summary: Dental enamel forms the outer cap of teeth and is the hardest substance found in vertebrates. This gene encodes the largest protein in the enamel matrix of developing teeth. The protein is involved in the mineralization and structural organization of enamel. Defects in this gene result in amelogenesis imperfect type 1C. [provided by RefSeq, Oct 2009]
• Locus ID: 10117