PTCH1 (NM_000264) Human 3' UTR Clone

CAT#: SC220803

3`UTR clone of patched homolog 1 (Drosophila) (PTCH1) transcript variant 1b for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PTCH1
• Synonyms: BCNS; NBCCS; PTC; PTC1; PTCH
• ACCN: NM_000264
• Insert Size: 3428 bp
• Sequence Data:
  Insert Sequence

  >SC220803 3'UTR clone of NM_000264
  The sequence shown below is from the reference sequence of NM_000264. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACGTGGAATGCGAGGAGAGGCCCCGGGGAAGCAGCTCCAACTGAGGGTGATTAAAATCTGAAGCAAAGAG
  GCCAAAGATTGGAAACCCCCCACCCCCACCTCTTTCCAGAACTGCTTGAAGAGAACTGGTTGGAGTTATG
  GAAAAGATGCCCTGTGCCAGGACAGCAGTTCATTGTTACTGTAACCGATTGTATTATTTTGTTAAATATT
  TCTATAAATATTTAAGAGATGTACACATGTGTAATATAGGAAGGAAGGATGTAAAGTGGTATGATCTGGG
  GCTTCTCCACTCCTGCCCCAGAGTGTGGAGGCCACAGTGGGGCCTCTCCGTATTTGTGCATTGGGCTCCG
  TGCCACAACCAAGCTTCATTAGTCTTAAATTTCAGCATATGTTGCTGCTGCTTAAATATTGTATAATTTA
  CTTGTATAATTCTATGCAAATATTGCTTATGTAATAGGATTATTTTGTAAAGGTTTCTGTTTAAAATATT
  TTAAATTTGCATATCACAACCCTGTGGTAGTATGAAATGTTACTGTTAACTTTCAAACACGCTATGCGTG
  ATAATTTTTTTGTTTAATGAGCAGATATGAAGAAAGCACGTTAATCCTGGTGGCTTCTCTAGGTGTCGTT
  GTGTGCGGTCCTCTTGTTTGGCTGTGCGTGTGAACACGTGTGTGAGTTCACCATGTACTGTACTGTGATT
  TTTTTTTTGTCTTGTTTTGTTTCTCTACACTGTCTGTAACCTGTAGTAGGCTCTGACCTAGTCAGGCTGG
  AAGCGTCAGGATATCTTTTCTTCGTGCTGGTGAGGGCTGGCCCTAAACATCCACCTAATCCTTTCAAATC
  AGCCCGGCAAAAGCTAGACTCTCCTCGTGTCTACGGCATCTCTTATGATCATTGGCTGCCATCCAGGACC
  CCAATTTGTGCTTCAGGGGGATAATCTCCTTCTCTCGGATCATTGTGATGGATGCTGGAACCTCAGGGTA
  TGGAGCTCACATCAGTTCATCATGGTGGGTGTTAGAGAATTCGGTGACATGCCTAGTGCTGAGCCTTGGC
  TGGGCCATGAGAGTCTGTATACTCTAAAAAGCATGCAGCATGGTGCCCCTCTTCTGACCAACACACACAC
  GACCCCTCCCCCAACACCCCCAAATTCAAGAGTGGATGTGGCCCTGTCACAGGTAGAAAAACCTATTTAG
  TTAATTCTTTCTTGGCCCACAGTCTCCCAGAAATGATGTTTTGAGTCCCTATAGTTTAAACTCCCTCTCT
  TAAATGGAGCAGCTGGTTGAGGCTTTCTAGATCTGTTTGCATCTTCTTTAAAACTAAGTGGTGAGCATGC
  ATTGTGGTGTAGAGGCAGGCATTATGTAGGATAAGAGCTCCGGGGGGATTCTTCATGCACCAGTGTTTAG
  GGTACGTGCTTCCTAAGTAAATCCAAACATTGTCTCCATCCTCCCCGTCATTAGTGCTCTTTCAATGTGA
  TGTGGGAAAGCAGGAGGATGGACACACCCCACTGAAAGATGTAGGCAGGGGCAGGTCTCTCAACCAGGCA
  TATTTTTAAAAGTTGCTTCTGTACTGGTTCTCTTCTTTTGCTCTGAGGTGTGGGCTCCCTCATCTCGTAA
  CCAGAGACCAGCACATGTCAGGGAAGCACCCAGTGTCGGCTCCCCATCCAAATCCACACCAGCACCTTGT
  TACAGACAAGAAGTCAGAGGAAAGGGCGGGGTCCCTGCAGGGCTGAAGCCTAAGCTACTGTGAGGCGCTC
  ACGAGTGGCAGCTCCTGTTACTCCCTTTTAAATTACCTGGGAAATCTTAACAGAAAGGTAATGGGCCCCC
  AGAAATACCCACAGCATAGTGACCTCAGACCCTGATACTCACCACAAAACTTTTAAGATGCTGATTGGGA
  GCCGCTTGTGGCTGCTGGGTGTGTGTGTGTGTGTGTGCGTGCGTGCGTGTGTGTGTGTCTCTGCTGGGGA
  CCCTGGCCACCCCCCTGCTGCTGTCTTGGTGCCTGTCACCCACATGGTCTGCCATCCTAACACCCAGCTC
  TGCTCAGAAAACGTCCTGCGTGGAGGAGGGATGATGCAGAATTCTGAAGTCGACTTCCCTCTGGCTCCTG
  GCGTGCCCTCGCTCCCTTCCTGAGCCCAGCTCGTGTTGCGCCGGAGGCTGCGCGGCCCCTGATTTCTGCA
  TGGTGTAGAACTTTCTCCAATAGTCACATTGGCAAAGGGAGAACTGGGGTGGGCGGGGGGTGGGGCTGGC
  AGGGAATTAGAATTTCTCTCTCTCTTTTAATAGTTTTATTTTGTCTGTCCTGTTTGTTCATTTGGATGTT
  TTAATTTTTAAAAAAAAAAAAACTTTGCTGATATTTATAATTTTGTATCATAAGAATGTTTTCCTCTACA
  GTATTTGTCATGCCAGTTTATAACAAAAAAAAATGCAGGGATTTTATTTCTATTGGAAACATTACAGCTA
  TGTTTTACTTTTGGACAGAATTTTTATTTGTATAGAGTGCTTACTAATGTTAAATAGTTCAGAGTATATA
  ACATTTACATTAAGGACTCATGGTAGGTTTTAGGGTAAGGAGTTTAAAGGAAATAAATATTCAAACTGGG
  TCTCATTGCCAATTTTGGTGGAAATGAGTTTGTGTCATTTCAATTACAAAGATAAAAGTATGCCATATAA
  TTTATTTATATGAAGATTTATTTTTGTAGTGTACATAGTAGTCATCAAGTCTTTTGACAGAAGTATATTT
  TTAAAGAATTTATATGTGATGAATCCATAATGTCTGGAACTTTGCTGAGACATGAGTGGGCACAGTTTTC
  ATTGTAAATTACAGCAAGGAAAGAAAATGTTTAACAGTGTTAAGAGAGTCAGAGCAGAGTGGATATTCAT
  GCGATTATGAAGTGTTTATTAGTTACCATTGGCGACCTAGCATGCTTCTCATTTCAAACCTTGGAAGGTG
  AAAATGTACAAACTCTCTAAATAATTAATGTTCAAACACTGATAGAAATTCTAACATGAATAAAAAATAA
  TATAACTTGTTGGTTATATCTTTGTTTGTAGAATGCTTTTTTTCTTTAAAATACTTGGGAGAGACAGTTA
  GTGTTGGAGCCATCAGAAACTGTTGTTTCATCTTGCTGACCTTGTGACACTCTTCTTTTTTGCTCTATCT
  GATGGAGAGAGTTTTAGGTTTTCCTCCTTTTTGTTTTGTATAAATAGTGGGTATAAGCATAGCTCTGTTA
  CATGGATGTATTACAAAGTGGGGGCTGAGCTTTTAGTGTGATCATCACCCAAATAGTGTACATTGTACCC
  ATTAATTAATTTCTTATCACCTGACCCTATTTTTGTTTTCTCATACCAAGAAACTTCCTAAGTTAACCAT
  CAAAATTAGTCCTTCTGTCATCTCTCTTAGCATACTTATTTCCCTTATGTGATGCCTAATAATGAGGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000264.3
• Summary: 'This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017]'
• Locus ID: 5727