Dclk1 (NM_001195539) Mouse Untagged Clone

CAT#: MC215927

Dclk1 (untagged) - Mouse doublecortin-like kinase 1 (Dclk1), transcript variant 6, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dclk1
• Synonyms: 1700113D08Rik; 2810480F11Rik; AI836758; Click-I; Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215927 representing NM_001195539
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAGAGCTCATAGAAGTTAATGGAACCCCTGGTAGTCAGCTCTCTACTCCACGCTCGGGCAAGTCAC
  CAAGTCCATCACCCACCAGCCCAGGAAGCCTGCGGAAGCAGAGGATCTCTCAGCATGGCGGCTCCTCGAC
  TTCACTTTCATCCACTAAAGTTTGCAGCTCAATGGATGAGAATGATGGCCCTGGGGAAGGTGATGAGCTT
  GGGAGAAGGCACAGCTTGCAGAGGGGATGGAGGAGGGAAGAGTCTGAGGAAGGCTTCCAGATTCCTGCCA
  CAATAACAGAGAGATACAAAGTCGGGAGAACAATAGGAGACGGAAATTTTGCTGTTGTCAAGGAATGTAT
  AGAGAGGTCGACTGCTCGGGAGTATGCCCTGAAAATCATCAAGAAAAGCAAATGCCGAGGCAAAGAGCAC
  ATGATCCAGAACGAGGTCTCCATCCTACGGAGGGTGAAGCACCCCAACATTGTCCTCCTGATTGAAGAGA
  TGGATGTGCCGACTGAACTGTATCTTGTAATGGAATTAGTGAAGGGTGGAGACCTTTTCGATGCCATCAC
  CTCCACTAGCAAATACACAGAGAGAGATGCCAGCGGGATGCTGTACAACCTGGCCAGCGCCATCAAATAC
  CTGCACAGCCTGAACATCGTCCACCGTGACATCAAGCCAGAGAATCTGCTGGTGTATGAGCACCAGGATG
  GCAGTAAGTCACTCAAGTTGGGTGACTTTGGCCTGGCCACAATTGTCGACGGCCCCCTGTACACAGTCTG
  TGGCACCCCAACATATGTGGCTCCAGAAATCATTGCAGAGACTGGATATGGCCTCAAGGTGGACATCTGG
  GCAGCTGGCGTGATCACTTATATCCTGCTGTGTGGCTTCCCTCCGTTCCGTGGAAGTGGGGATGACCAGG
  AGGTGCTTTTTGACCAGATCTTGATGGGCCAAGTGGACTTTCCATCTCCGTATTGGGACAATGTGTCAGA
  TTCTGCTAAGGAGCTCATCAACATGATGCTGTTGGTTAACGTGGACCAGAGATTTTCAGCCGTGCAGGTC
  CTTGAGCATCCCTGGGTTAATGATGATGGTCTCCCAGAAAATGAGCATCAGCTGTCAGTAGCTGGCAAAA
  TCAAGAAGCATTTCAACACAGGCCCCAAGCCGAGCAGCACTGCAGCAGGAGTTTCTGTAATAGCAACCAC
  CGCTCTTGATAAGGAGAGGCAGGTTTTCCGACGAAGACGCAACCAGGATGTGAGGAGCCGGTACAAGGCG
  CAGCCAGCTCCACCGGAATTGAACTCGGAATCGGAGGACTACTCCCCCAGCTCCTCTGAGACTGTTCGCT
  CCCCCAATTCGCCCTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001195539
• ORF Size: 1350 bp
• Insert Size: 1350
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_001195539.1, NP_001182468.1
• RefSeq Size: 7004
• RefSeq ORF: 1350
• Locus ID: 13175
• Gene Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the biological validity of some variants has not been determined. These variants encode different isoforms, which are differentially expressed and have different kinase activities. [provided by RefSeq, Sep 2010]
  Transcript Variant: This variant (6, also known as DCLK-short-C or DCK-beta2-R) is produced from the 3' promoter. It lacks several 5' exons but has an alternate 5' exon, and has an additional in-frame segment in the CDS, as compared to variant 5. The resulting isoform (6) has a shorter and different N-terminus, and lacks doublecortin domains but has an additional Arg-rich segment, as compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.