Col4a2 (NM_009932) Mouse Untagged Clone

CAT#: MC224842

Col4a2 (untagged) - Mouse collagen, type IV, alpha 2 (Col4a2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Col4a2
• Synonyms: Col4a-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224842 representing NM_009932
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGAGTGCGGTTCAAAGCGTCTGGCCCTCCCCTGCGAGGGTGGCTGCTGCTAGCAACTGTGACAG
  TGGGACTCCTGGCTCAGAGCGTCTTGGGGGGTGTGAAGAAGTTGGATGTGCCCTGCGGAGGGAGAGACTG
  CAGTGGGGGTTGCCAGTGCTACCCGGAGAAAGGAGCAAGGGGTCAGCCAGGGGCAGTGGGCCCCCAGGGG
  TACAATGGCCCCCCAGGGTTGCAAGGATTCCCAGGACTACAGGGCCGCAAAGGAGACAAGGGCGAACGGG
  GAGTTCCTGGACCAACTGGACCAAAAGGAGATGTGGGAGCGAGAGGCGTCTCTGGATTCCCCGGTGCAGA
  TGGAATTCCTGGGCATCCAGGGCAAGGCGGACCCCGAGGAAGGCCAGGCTACGATGGTTGCAACGGAACC
  AGGGGAGACGCGGGTCCACAGGGGCCCTCTGGATCTGGGGGCTTCCCTGGCCTCCCTGGGCCCCAAGGAC
  CCAAGGGGCAGAAAGGCGAGCCTTACGCACTCTCTAAAGAAGACCGTGACAAATACCGGGGTGAACCTGG
  AGAGCCTGGCCTGGTTGGCTACCAGGGACCTCCTGGCCGCCCAGGGCCCATAGGACAGATGGGTCCCATG
  GGAGCACCTGGAAGACCGGGACCACCGGGACCCCCTGGACCCAAGGGACAACCAGGCAACCGAGGGCTCG
  GTTTTTACGGACAGAAGGGTGAAAAGGGTGACATAGGACAGCCAGGACCCAACGGGATCCCATCTGACAT
  CACACTTGTTGGGCCCACAACATCAACGATCCACCCGGATCTGTACAAGGGTGAAAAAGGAGACGAAGGA
  GAGCAAGGGATACCCGGCGTAATCTCAAAAGGCGAGGAAGGCATCATGGGGTTCCCAGGAATACGGGGTT
  TTCCTGGCCTTGATGGAGAAAAAGGAGTCGTAGGACAGAAAGGAAGCAGAGGCCTGGATGGTTTCCAAGG
  CCCCAGTGGACCTCGAGGACCCAAGGGAGAACGGGGGGAACAAGGACCCCCAGGACCCTCTGTCTACTCG
  CCCCATCCATCCCTGGCAAAAGGTGCCCGAGGTGACCCAGGATTCCAAGGTGCTCATGGGGAGCCAGGAA
  GCCGAGGCGAACCGGGAGAGCCTGGAACCGCGGGTCCTCCAGGCCCATCCGTCGGAGATGAAGATTCAAT
  GAGAGGCCTTCCAGGGGAGATGGGACCCAAAGGCTTCTCAGGAGAACCAGGGTCCCCTGCACGCTATCTT
  GGCCCACCAGGAGCAGATGGAAGACCAGGTCCCCAAGGAGTCCCTGGACCTGCAGGCCCACCTGGACCAG
  ATGGCTTCCTGTTTGGCCTGAAAGGATCAGAAGGGAGAGTGGGCTACCCGGGACCTTCAGGTTTCCCAGG
  AACACGAGGGCAGAAAGGATGGAAAGGTGAAGCTGGAGACTGCCAATGTGGCCAGGTCATCGGGGGTCTT
  CCGGGACTGCCAGGACCCAAAGGTTTTCCCGGTGTCAATGGGGAGCTCGGAAAGAAAGGCGACCAAGGAG
  ACCCAGGCCTGCACGGTATCCCTGGGTTCCCAGGATTCAAGGGAGCCCCGGGCGTTGCTGGAGCTCCTGG
  ACCCAAAGGAATAAAGGGAGACTCTAGAACAATCACCACCAAAGGGGAGCGAGGACAGCCAGGTATCCCT
  GGTGTACACGGAATGAAAGGTGATGACGGCGTCCCAGGACGGGATGGGCTGGATGGATTCCCTGGCCTGC
  CTGGCCCCCCGGGCGATGGCATCAAAGGCCCTCCAGGGGATGCAGGTCTCCCTGGTGTACCCGGAACCAA
  GGGCTTTCCAGGAGACATCGGTCCTCCAGGACAGGGCTTACCAGGTCCGAAAGGCGAGCGTGGTTTCCCT
  GGAGACGCTGGGTTGCCTGGACCTCCGGGCTTCCCTGGTCCTCCAGGTCCCCCAGGAACCCCAGGACAGA
  GAGATTGTGACACGGGTGTGAAAAGACCTATCGGAGGCGGCCAGCAAGTGGTCGTTCAGCCAGGTTGCAT
  TGAAGGCCCCACAGGATCACCTGGCCAGCCAGGACCCCCAGGCCCCACAGGTGCCAAAGGCGTCAGAGGA
  ATGCCAGGATTCCCAGGAGCTTCTGGAGAACAAGGGCTCAAGGGCTTTCCAGGAGACCCAGGTCGAGAAG
  GCTTCCCAGGGCCCCCAGGGTTCATGGGACCCCGAGGATCCAAAGGTACCACAGGCCTCCCTGGCCCAGA
  TGGACCCCCAGGCCCCATTGGACTTCCAGGACCAGCTGGACCCCCTGGGGACAGAGGGATTCCAGGAGAA
  GTGCTAGGGGCCCAGCCTGGGACACGAGGAGATGCTGGACTTCCAGGACAGCCTGGGCTGAAAGGGCTCC
  CAGGAGAAACAGGAGCACCGGGATTCAGAGGAAGCCAAGGGATGCCCGGGATGCCAGGGCTTAAAGGTCA
  GCCAGGCTTCCCAGGACCTTCGGGACAGCCAGGACAGTCTGGGCCCCCAGGACAACATGGATTCCCAGGA
  ACTCCTGGCCGAGAGGGGCCTTTGGGGCAGCCAGGCTCCCCTGGCCTTGGAGGTTTGCCTGGAGACAGGG
  GCGAGCCTGGTGACCCTGGTGTCCCTGGCCCTGTGGGCATGAAAGGTCTGTCTGGGGACAGAGGTGACGC
  CGGTATGTCAGGCGAGCGTGGTCACCCTGGAAGCCCTGGATTTAAAGGAATGGCAGGGATGCCTGGTATC
  CCTGGGCAGAAAGGTGATCGAGGGTCGCCTGGAATGGACGGTTTCCAGGGCATGTTGGGACTTAAGGGAA
  GACAGGGCTTCCCAGGGACCAAAGGAGAAGCTGGATTTTTTGGAGTTCCTGGTTTGAAGGGCCTGCCTGG
  CGAGCCAGGGGTTAAAGGAAACCGAGGAGACCGGGGGCCCCCAGGACCACCTCCTCTCATCCTGCCGGGG
  ATGAAGGACATCAAGGGAGAAAAGGGAGATGAAGGACCAATGGGCCTGAAAGGGTATCTGGGCTTAAAAG
  GCATCCAAGGAATGCCCGGAGTCCCCGGAGTGTCTGGATTCCCTGGGCTACCTGGAAGGCCTGGCTTCAT
  CAAAGGAGTCAAGGGAGACATCGGAGTCCCTGGCACACCAGGCTTGCCGGGATTCCCTGGGGTGTCTGGC
  CCTCCTGGAATTACCGGGTTTCCAGGATTCACAGGCAGCCGGGGCGAGAAGGGTACTCCAGGAGTAGCAG
  GAGTTTTTGGCGAGACCGGCCCTACTGGGGACTTTGGTGACATTGGGGACACTGTGGACTTACCAGGGAG
  CCCAGGCCTGAAGGGGGAACGGGGCATCACGGGAATTCCAGGTCTCAAGGGATTCTTTGGAGAGAAAGGA
  GCTGCGGGTGACATTGGATTTCCTGGGATAACGGGCATGGCTGGAGCTCAGGGCTCTCCTGGACTTAAAG
  GACAGACAGGCTTTCCAGGGCTCACAGGGCTGCAAGGGCCACAGGGAGAGCCTGGACGGATCGGAATACC
  CGGTGACAAGGGTGATTTCGGCTGGCCAGGCGTACCAGGTCTACCAGGTTTTCCTGGGATCCGGGGCATC
  AGCGGATTGCACGGCCTGCCAGGCACCAAAGGCTTCCCTGGATCACCAGGTGTTGATGCTCATGGAGACC
  CAGGGTTCCCAGGCCCCACTGGAGACAGGGGTGACCGGGGAGAGGCCAACACACTTCCAGGCCCCGTGGG
  AGTTCCAGGGCAGAAAGGGGAGCGGGGAACCCCAGGGGAACGTGGCCCAGCTGGAAGCCCAGGACTTCAG
  GGTTTCCCTGGTATCTCTCCACCATCCAACATCTCTGGGTCACCTGGTGATGTAGGCGCACCAGGAATAT
  TTGGCTTGCAAGGCTACCAAGGCCCTCCAGGACCACCTGGGCCAAACGCACTTCCTGGAATCAAAGGAGA
  TGAGGGGAGCTCTGGAGCTGCAGGATTCCCTGGACAGAAAGGATGGGTTGGGGACCCAGGGCCCCAGGGC
  CAGCCTGGTGTACTCGGTCTTCCGGGGGAGAAAGGGCCCAAGGGTGAACAAGGATTCATGGGCAACACCG
  GGCCCTCTGGGGCCGTGGGTGACAGAGGCCCCAAAGGACCCAAAGGCGACCAAGGATTCCCAGGTGCTCC
  TGGCTCTATGGGGTCCCCAGGAATTCCTGGCATCCCCCAGAAGATTGCTGTCCAGCCTGGAACGCTGGGT
  CCCCAGGGCAGGAGAGGCCTTCCTGGGGCCCTGGGAGAGATAGGGCCGCAGGGCCCTCCTGGAGATCCAG
  GCTTCCGTGGGGCCCCAGGCAAGGCTGGGCCTCAGGGCAGAGGTGGCGTGTCTGCTGTTCCAGGGTTCCG
  GGGAGACCAAGGGCCCATGGGACACCAGGGTCCAGTTGGCCAGGAAGGGGAGCCAGGCCGTCCAGGGAGC
  CCAGGCCTGCCAGGGATGCCAGGCCGCAGTGTGAGCATCGGCTACCTCCTGGTGAAGCACAGCCAAACGG
  ACCAGGAACCCATGTGCCCTGTGGGCATGAACAAGCTCTGGAGTGGGTACAGCCTGCTATATTTTGAGGG
  CCAGGAGAAAGCGCACAACCAGGACCTAGGACTGGCAGGCTCCTGCCTGGCACGCTTCAGCACCATGCCT
  TTCCTGTACTGCAATCCGGGTGACGTCTGCTACTATGCCAGCCGCAACGACAAGTCCTACTGGCTCTCCA
  CCACGGCCCCTCTGCCCATGATGCCCGTGGCTGAGGAGGAAATCAAGCCCTACATCAGCCGCTGCTCTGT
  GTGCGAGGCTCCGGCCGTGGCCATTGCCGTGCACAGCCAGGATACCTCTATACCCCACTGCCCGGCTGGG
  TGGCGGAGTTTGTGGATCGGATATTCATTCCTCATGCACACTGCAGCCGGGGATGAAGGCGGTGGCCAGT
  CACTGGTGTCGCCGGGCAGCTGTCTAGAGGACTTCCGTGCAACGCCATTTATCGAGTGTAACGGGGGCCG
  TGGTACCTGCCACTACTTCGCTAACAAGTACAGCTTCTGGCTGACCACGATCCCAGAGCAGAACTTCCAG
  AGCACACCATCCGCTGACACGCTCAAGGCTGGCCTCATCCGCACGCACATCAGCCGCTGCCAAGTGTGCA
  TGAAGAATCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009932
• ORF Size: 5124 bp
• Insert Size: 5124
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_009932.4, NP_034062.3
• RefSeq Size: 6467
• RefSeq ORF: 5124
• Locus ID: 12827
• Gene Summary: This gene encodes the alpha-2 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of alpha-1 and alpha-2 subunits that assembles into a type IV collagen network. Canstatin, a peptide derived fom the C-terminus of the collagen chain, is a matrikine that has been shown to inhibit angiogenesis. Homozygous knockout mice for this gene exhibit impaired basement membrane integrity and embryonic lethality. This gene shares a bi-directional promoter with a related gene on chromosome 8. [provided by RefSeq, Nov 2015]