Yaf2 (BC002192) Mouse Tagged ORF Clone

CAT#: MG201598

Yaf2 (GFP-tagged) - Mouse YY1 associated factor 2 (cDNA clone MGC:7415 IMAGE:3488691)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Yaf2
• Synonyms: 2810021M11Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201598 representing BC002192
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACAAGAAGAGCCCCACCAGGCCGAAGCGGCAGCCGAAGCCCGCCTCGGATGAGGGCTACTGGG
  ACTGCAGTGTCTGCACCTTCCGGAACAGCGCCGAGGCCTTCAAGTGCATGATGTGCGATGTGCGGAAGGG
  CACCTCCACCCGGAAGCCACGCCCTGTCTCCCAGCTGGTCGCACAGCAGGTCACGCAGCAGTTCGTGCCC
  CCTACCCAGTCGAAGAAAGAGAAGAAAGACCGAGTAGAGAAGGACAAGAGTGAGAAGGAAGCTGCTAGCA
  AAAAGAACTGTCACAAGAAAACCAGACCAAGATTAAAAAATGTGGATCGGAGTAGCGCTCAGCATTTGGA
  AGTCACTGTTGGAGATCTAACAGTCATTATCACAGACTTTAAGGAGAAAGCCAAGTCAGCGCCTGCGTCC
  AGCGCTGCTGGCGATCAGCACAGCCAGGGCAGCTGCAGCTCGGACAGCACTGAGCGGGGCGTGTCCAGGT
  CCTCCTCGCCGCGGGGAGAAGCCTCCTCCCTCAACGGAGAGTCTCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201598 representing BC002192
  Red=Cloning site Green=Tags(s)
  
  MGDKKSPTRPKRQPKPASDEGYWDCSVCTFRNSAEAFKCMMCDVRKGTSTRKPRPVSQLVAQQVTQQFVP
  PTQSKKEKKDRVEKDKSEKEAASKKNCHKKTRPRLKNVDRSSAQHLEVTVGDLTVIITDFKEKAKSAPAS
  SAAGDQHSQGSCSSDSTERGVSRSSSPRGEASSLNGESH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC002192
• ORF Size: 539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC002192, AAH02192
• RefSeq Size: 1691
• RefSeq ORF: 539
• Locus ID: 67057
• Gene Summary: Binds to MYC and inhibits MYC-mediated transactivation. Also binds to MYCN and enhances MYCN-dependent transcriptional activation. Increases calpain 2-mediated proteolysis of YY1 in vitro. Component of the E2F6.com-1 complex, a repressive complex that methylates 'Lys-9' of histone H3, suggesting that it is involved in chromatin-remodeling. [UniProtKB/Swiss-Prot Function]