Cldn15 (NM_021719) Mouse Tagged ORF Clone

CAT#: MG202708

  • TrueORF®

Cldn15 (GFP-tagged) - Mouse claudin 15 (Cldn15)


  "NM_021719" in other vectors (4)

Reconstitution Protocol

USD 460.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Cldn15
Synonyms 2210009B08Rik; BB107105
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG202708 representing NM_021719
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGTAGCTGTGGAGACCTTCGGCTTCTTCATGTCAGCCCTGGGACTGCTGATGCTGGGGTTGACCC
TTTCAAACAGCTACTGGAGAGTGTCTACGGTCCATGGCAACGTCATCACCACCAACACCATCTTTGAGAA
CCTGTGGTACAGCTGTGCCACCGACTCCCTGGGAGTCTCCAACTGCTGGGACTTTCCGTCCATGCTGGCC
CTCTCTGGGTATGTCCAGGGCTGCCGGGCTCTCATGATCACCGCCATCCTCCTGGGCTTCCTGGGCCTCT
TTCTAGGCATGGTGGGACTCCGCTGCACCAACGTGGGCAACATGGATCTCTCCAAGAAGGCCAAGCTGCT
GGCCATTGCAGGGACCCTCCACATACTTGCTGGAGCCTGTGGGATGGTGGCTATCTCGTGGTACGCCGTC
AACATCACTACTGACTTCTTCAACCCACTGTATGCTGGAACCAAGTATGAACTGGGCCCCGCCCTCTACT
TGGGCTGGAGTGCCTCCCTGCTCTCCATCCTGGGCGGCATCTGTGTCTTCTCCACCTGCTGCTGTTCCTC
CAAGGAGGAACCAGCCACCAGGGCTGGGCTTCCCTACAAGCCTTCTACGGTTGTGATACCCCGTGCCACC
TCGGATGAGAGTGACATCAGCTTCGGTAAATATGGCAAAAACGCATACGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG202708 representing NM_021719
Red=Cloning site Green=Tags(s)

MSVAVETFGFFMSALGLLMLGLTLSNSYWRVSTVHGNVITTNTIFENLWYSCATDSLGVSNCWDFPSMLA
LSGYVQGCRALMITAILLGFLGLFLGMVGLRCTNVGNMDLSKKAKLLAIAGTLHILAGACGMVAISWYAV
NITTDFFNPLYAGTKYELGPALYLGWSASLLSILGGICVFSTCCCSSKEEPATRAGLPYKPSTVVIPRAT
SDESDISFGKYGKNAYV

TRTRRLE - GFP Tag - V
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021719
ORF Size 681 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_021719.1, NP_068365.1
RefSeq Size 1847
RefSeq ORF 684
Locus ID 60363
Gene Summary This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This protein increases permeability for sodium ions in anion-selective epithelial cell sheets. The gene deficiency leads to megaintestine and decreases in intestinal epithelial paracellular ion permeability. This gene is a direct target for hepatocyte-nuclear-factor-4alpha, a mediator of ion epithelial transport, and is down-modulated in inflammatory bowel disease. [provided by RefSeq, Aug 2010]

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