Atp5f1 (BC087882) Mouse Tagged ORF Clone

CAT#: MG203291

Atp5f1 (GFP-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1 (cDNA clone MGC:107150

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atp5f1
• Synonyms: C76477
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203291 representing BC087882
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCCCGGGTGGTGCTTTCTGCTGCCGCCACAGCGGCCCCGTGTCTGAAGAACGCGGCCGCCCTAG
  GTCCAGGGGTATTACAGGCAACAAGGGCCTTTCACACAGGACAGCCTCGCCTTGCCCCTCTACCACCTCT
  TCCTGAATATGGAGGAAAAGTGCGTCTTGGGCTGATTCCTGAGGAATTTTTCCAGTTCCTTTACCCTAAG
  ACTGGTGTAACAGGACCTTATGTGCTTGGAACTGGACTTAGCTTGTATTTTCTATCCAAAGAAATATATG
  TGATTACCCCAGAGACCTTCTCTACCATATCAGTAGTAGGGTTGATAGTCTATGTGATTAAGAAATATGG
  CGCCTCTTTTGGAGAATTTATTGACAAACTTAATGAGGAAAAAATTGCTCAACTAGAAGAAGTAAAGCAG
  TCGAGCATGAAACAAATCCAGGATGCAATCGACATGGAGAAGGCACAGCAGGCACTGGTTCAGAAGCGCC
  ATTACCTCTTCGATGTGCAGAGGAATAACATTGCCCTGGCCTTGGAGGTCACTTACCGGGAACGGCTACA
  TAAAGCATATAAGGAGGTAAAGAATCGCCTGGACTACCACATCTCTGTACAGAACATGATGCGTCGCAAG
  GAGGAAGAACACATGATAGACTGGGTAGAAAAGCATGTGGTGAAGAGCATTTCTGTACAGCAGGAAAAGG
  AGACCATTGCCAAGTGCATTGAAGATCTAAAGCTGCTTGCAAAGAAGGCTCAAGCTCAGCCAATTATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203291 representing BC087882
  Red=Cloning site Green=Tags(s)
  
  MLSRVVLSAAATAAPCLKNAAALGPGVLQATRAFHTGQPRLAPLPPLPEYGGKVRLGLIPEEFFQFLYPK
  TGVTGPYVLGTGLSLYFLSKEIYVITPETFSTISVVGLIVYVIKKYGASFGEFIDKLNEEKIAQLEEVKQ
  SSMKQIQDAIDMEKAQQALVQKRHYLFDVQRNNIALALEVTYRERLHKAYKEVKNRLDYHISVQNMMRRK
  EEEHMIDWVEKHVVKSISVQQEKETIAKCIEDLKLLAKKAQAQPIM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC087882
• ORF Size: 770 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC087882, AAH87882
• RefSeq Size: 1487
• RefSeq ORF: 770
• Locus ID: 11950
• Gene Summary: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. [UniProtKB/Swiss-Prot Function]