Cntfr (BC046974) Mouse Tagged ORF Clone
CAT#: MG205731
- TrueORF®
Cntfr (GFP-tagged) - Mouse ciliary neurotrophic factor receptor (cDNA clone MGC:54584 IMAGE:6389656)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Cntfr |
Synonyms | Cntfralpha |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205731 representing BC046974
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTTCTGTCCCATGGGCCTGCTGTGCTGTGCTTGCCGCTGCCGCCGCCGCTGTCTACACGCAGA AACACAGTCCACAGGAGGCACCCCACGTGCAGTATGAGCGTCTGGGCGCAGATGTGACGCTGCCGTGTGG GACAGCGAGCTGGGACGCAGCTGTGACATGGAGGGTAAACGGGACAGATCTGGCCCCTGACCTGCTCAAC GGCTCTCAGCTGATACTGCGAAGCTTAGAACTGGGCCACAGTGGCCTATACGCCTGTTTCCACCGTGACT CCTGGCACCTGCGCCACCAAGTCCTTCTACATGTGGGTTTGCCGCCGCGGGAGCCTGTGCTCAGCTGCCG CTCCAACACTTACCCCAAGGGCTTCTACTGCAGCTGGCACCTGCCCACCCCCACCTACATCCCCAATACC TTCAATGTGACTGTGCTGCATGGCTCCAAAATTATGGTCTGTGAGAAGGACCCAGCCCTCAAGAACCGCT GCCACATTCGGTACATGCACCTGTTCTCAACCATCAAGTACAAGGTCTCCATAAGTGTCAGCAACGCCTT GGGTCACAACACTACGGCCATCACCTTTGACGAATTCACCATTGTGAAGCCTGATCCTCCAGAAAACGTG GTGGCCCGGCCAGTGCCCAGCAACCCCCGTCGGCTGGAGGTGACATGGCAGACACCCTCAACTTGGCCCG ACCCTGAATCCTTTCCTCTCAAGTTCTTCCTGCGCTACCGGCCTCTCATCCTGGACCAATGGCAGCATGT GGAGCTCTCGGATGGCACAGCACACACCATCACAGATGCCTACGCAGGGAAGGAATACATCATCCAGGTG GCAGCCAAGGACAATGAGATTGGGACATGGAGTGACTGGAGCGTGGCTGCTCACGCCACACCCTGGACTG AGGAACCTCGGCATCTCACAACAGAAGCCCAGGCCCCCGAGACCACGACCAGCACCACCAGCTCATTGGC ACCCCCACCCACCACGAAGATCTGTGACCCTGGGGAGCTTGGCAGCGGCGGAGGACCCTCCATACTCTTC TTGACCAGTGTCCCAGTCACTCTGGTCCTGGCTGCCGCTGCTGCCACAGCCAACAATCTCCTGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205731 representing BC046974
Red=Cloning site Green=Tags(s) MAASVPWACCAVLAAAAAAVYTQKHSPQEAPHVQYERLGADVTLPCGTASWDAAVTWRVNGTDLAPDLLN GSQLILRSLELGHSGLYACFHRDSWHLRHQVLLHVGLPPREPVLSCRSNTYPKGFYCSWHLPTPTYIPNT FNVTVLHGSKIMVCEKDPALKNRCHIRYMHLFSTIKYKVSISVSNALGHNTTAITFDEFTIVKPDPPENV VARPVPSNPRRLEVTWQTPSTWPDPESFPLKFFLRYRPLILDQWQHVELSDGTAHTITDAYAGKEYIIQV AAKDNEIGTWSDWSVAAHATPWTEEPRHLTTEAQAPETTTSTTSSLAPPPTTKICDPGELGSGGGPSILF LTSVPVTLVLAAAAATANNLLI TRTRRLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC046974 |
ORF Size | 1118 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | BC046974, AAH46974 |
RefSeq Size | 2013 |
RefSeq ORF | 1118 |
Locus ID | 12804 |
Gene Summary | This gene encodes the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor that triggers the assembly of a trimolecular complex upon binding to CNTF, and initiate a downstream signaling process. The encoded preproprotein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-linked cell surface protein. Mice lacking the encoded protein die shortly after birth and exhibit a reduction of motoneuron number at birth. The transgenic disruption of this gene specifically in the skeletal muscle followed by a peripheral nerve lesion impairs motor neuron axonal regeneration across the lesion site. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015] |
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