Cntfr (BC050928) Mouse Tagged ORF Clone

CAT#: MG205753

Cntfr (GFP-tagged) - Mouse ciliary neurotrophic factor receptor (cDNA clone MGC:61332 IMAGE:6415187)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cntfr
• Synonyms: Cntfralpha
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205753 representing BC050928
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTTCTGTCCCATGGGCCTGCTGTGCTGTGCTTGCCGCTGCCGCCGCCGCTGTCTACACGCAGA
  AACACAGTCCACAGGAGGCACCCCACGTGCAGTATGAGCGTCTGGGCGCAGATGTGACGCTGCCGTGTGG
  GACAGCGAGCTGGGACGCAGCTGTGACATGGAGGGTAAACGGGACAGATCTGGCCCCTGACCTGCTCAAC
  GGCTCTCAGCTGATACTGCGAAGCTTAGAACTGGGCCACAGTGGCCTATACGCCTGTTTCCACCGTGACT
  CCTGGCACCTGCGCCACCAAGTCCTTCTACATGTGGGTTTGCCGCCGCGGGAGCCTGTGCTCAGCTGCCG
  CTCCAACACTTACCCCAAGGGCTTCTACTGCAGCTGGCACCTGCCCACCCCCACCTACATCCCCAATACC
  TTCAATGTGACTGTGCTGCATGGCTCCAAAATTATGGTCTGTGAGAAGGACCCAGCCCTCAAGAACCGCT
  GCCACATTCGGTACATGCACCTGTTCTCAACCATCAAGTACAAGGTCTCCATAAGTGTCAGCAACGCCTT
  GGGTCACAACACTACGGCCATCACCTTTGACGAATTCACCATTGTGAAGCCTGATCCTCCAGAAAACGTG
  GTGGCCCGGCCAGTGCCCAGCAACCCCCGTCGGCTGGAGGTGACATGGCAGACACCCTCAACTTGGCCCG
  ACCCTGAATCCTTTCCTCTCAAGTTCTTCCTGCGCTACCGGCCTCTCATCCTGGACCAATGGCAGCATGT
  GGAGCTCTCGGATGGCACAGCACACACCATCACAGATGCCTACGCAGGGAAGGAATACATCATCCAGGTG
  GCAGCCAAGGACAATGAGATTGGGACATGGAGTGACTGGAGCGTGGCTGCTCACGCCACACCCTGGACTG
  AGGAACCTCGGCATCTCACAACAGAAGCCCAGGCCCCCGAGACCACGACCAGCACCACCAGCTCATTGGC
  ACCCCCACCCACCACGAAGATCTGTGACCCTGGGGAGCTTGGCAGCGGCGGAGGACCCTCCATACTCTTC
  TTGACCAGTGTCCCAGTCACTCTGGTCCTGGCTGCCGCTGCTGCCACAGCCAACAATCTCCTGATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205753 representing BC050928
  Red=Cloning site Green=Tags(s)
  
  MAASVPWACCAVLAAAAAAVYTQKHSPQEAPHVQYERLGADVTLPCGTASWDAAVTWRVNGTDLAPDLLN
  GSQLILRSLELGHSGLYACFHRDSWHLRHQVLLHVGLPPREPVLSCRSNTYPKGFYCSWHLPTPTYIPNT
  FNVTVLHGSKIMVCEKDPALKNRCHIRYMHLFSTIKYKVSISVSNALGHNTTAITFDEFTIVKPDPPENV
  VARPVPSNPRRLEVTWQTPSTWPDPESFPLKFFLRYRPLILDQWQHVELSDGTAHTITDAYAGKEYIIQV
  AAKDNEIGTWSDWSVAAHATPWTEEPRHLTTEAQAPETTTSTTSSLAPPPTTKICDPGELGSGGGPSILF
  LTSVPVTLVLAAAAATANNLLI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC050928
• ORF Size: 1118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC050928, AAH50928
• RefSeq Size: 2017
• RefSeq ORF: 1118
• Locus ID: 12804
• Gene Summary: This gene encodes the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor that triggers the assembly of a trimolecular complex upon binding to CNTF, and initiate a downstream signaling process. The encoded preproprotein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-linked cell surface protein. Mice lacking the encoded protein die shortly after birth and exhibit a reduction of motoneuron number at birth. The transgenic disruption of this gene specifically in the skeletal muscle followed by a peripheral nerve lesion impairs motor neuron axonal regeneration across the lesion site. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015]