Ttyh1 (BC046310) Mouse Tagged ORF Clone

CAT#: MG207183

Ttyh1 (GFP-tagged) - Mouse tweety homolog 1 (Drosophila) (cDNA clone MGC:54527 IMAGE:6307420)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ttyh1
• Synonyms: tty
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207183 representing BC046310
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCACCCCCGGGCTACCGACCCTCTGCCTGGGTGCATCTCCTCCACCAGCTGCCCCGCGCCGACT
  TCCAGCTCCGCCCGGTGCCCAGCGGCTTTGCTCCCCGCGACCAAGAGTACCAGCAGGCCCTGCTGCTGGT
  GGCAGCCTTGGCTGGGCTTGGTTTAGGCCTGAGCCTCATCTTCATCGCTGTCTACCTCATCCGCTTCTGC
  TGCTGCAGGCCTCCAGAACCCCATGGGGCCAAGAGTCCCCCACCCGGAGGAGGTTGCGTCACCTGGAGCT
  GCATTGCTGCCCTTCTCGTGGGCTGTGCCGGCATTGGCATTGGTTTCTATGGTAACAGCGAGACCAGCGA
  TGGGGTGTCCCAGCTCAGCTCAGCACTGCTGCACGCCAACCACACGCTCAGCACCATCGATGACGTGGTG
  CTGGAAACGGTGGAGAGGCTGGGTGAGGCAGTGAAGACGGAGCTGACCACCCTGGAGGAAGTGCTGTCAG
  TCCGCATGGAGCTGGTGGCTGCCACCAGGGGAGCCCGAAGGCAGGCTGAAGCTGCTGCTCAGTACCTGCA
  AGGACTGGCCTTCTGGCAAGGAGTGTCCCTGAGCCCCGTACAGGTGGCTGAAGATGTGACTTTCGTGGAG
  GAGTACAGGTGGCTGGCCTATGTCCTCCTGCTGCTCTTGGTGCTTCTGGTTTGTCTCTTCACCCTCCTGG
  GCCTGGCAAAGCAGAGCAAATGGCTGGTGGTTGTGATGACTGCCATGAGTCTCCTGGTGCTTGTCCTGAG
  CTGGGGTTCTATGGGCCTAGAGGCTGCCACAGCTGTGGGCCTCAGTGACTTCTGCTCCAACCCAGACACC
  TATGTGCTGAACCTGACCCAGGAGGAGACAGGGCTCAGCTCAGACATCCTCAGCTATTATTTCCTGTGCA
  ACCAGGCGGTCTCCAACCCCTTCCAACAGAGGCTGACGCTGTCCCAGCGTGCTCTAGCCAGCATCCACTC
  TCAGCTGCAGGGCCTGGAGCGGGAAGCTATCCCTCAATTTTCTGCTGCACAGAAGCCCTTGTTATCCTTG
  GAGGAGACACTGAATGTGACAGAAAGAAGCTTCCACCAGTTGGTGGCGCTGCTGCACTGCCGCAGTCTGC
  ACAAGGACTATGGCTCAGCACTCCGAGGCCTGTGTGAAGATGCCCTAGAGGGCCTACTGTTCCTGATGCT
  CTTTTCTCTCCTGTCTGCGGGGGCCCTAGCCACCACTCTGTGCAGCCTGCCCCGCGCCTGGGCCCTCTTC
  CCACCCAGTGATGACTACGATGACACAGATGACGATGACCCTTTCAACCCTCAGGAATCCAAACGCTTTG
  TGCAGTGGCAGTCTTCCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207183 representing BC046310
  Red=Cloning site Green=Tags(s)
  
  MGAPPGYRPSAWVHLLHQLPRADFQLRPVPSGFAPRDQEYQQALLLVAALAGLGLGLSLIFIAVYLIRFC
  CCRPPEPHGAKSPPPGGGCVTWSCIAALLVGCAGIGIGFYGNSETSDGVSQLSSALLHANHTLSTIDDVV
  LETVERLGEAVKTELTTLEEVLSVRMELVAATRGARRQAEAAAQYLQGLAFWQGVSLSPVQVAEDVTFVE
  EYRWLAYVLLLLLVLLVCLFTLLGLAKQSKWLVVVMTAMSLLVLVLSWGSMGLEAATAVGLSDFCSNPDT
  YVLNLTQEETGLSSDILSYYFLCNQAVSNPFQQRLTLSQRALASIHSQLQGLEREAIPQFSAAQKPLLSL
  EETLNVTERSFHQLVALLHCRSLHKDYGSALRGLCEDALEGLLFLMLFSLLSAGALATTLCSLPRAWALF
  PPSDDYDDTDDDDPFNPQESKRFVQWQSSI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC046310
• ORF Size: 1352 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC046310, AAH46310
• RefSeq Size: 2092
• RefSeq ORF: 1352
• Locus ID: 57776
• Gene Summary: This gene encodes a member of the Tweety family of membrane proteins. Members of this family contain five predicted transmembrane regions that are arranged in a characteristic pattern. In mouse, the protein is predominantly localized to the endoplasmic reticulum and displays calcium binding activity. Targeted knock out of this gene results in early embryonic lethality prior to the blastocyst stage. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]