Dync1li2 (NM_001013380) Mouse Tagged ORF Clone

CAT#: MG207876

Dync1li2 (GFP-tagged) - Mouse dynein, cytoplasmic 1 light intermediate chain 2 (Dync1li2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dync1li2
• Synonyms: AA409702; C920003I06; Dncli2; Dnclic2; LIC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207876 representing NM_001013380
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGGTGGGGGTGGAGAAGAAGCTGCTGCTGGGTCCCAACGGGCCCGCAGTGGCGGCCGCCGGCG
  ACCTGACAAGTGAAGAGGAGGAAGGCCAGAGTCTATGGTCTTCGATCTTGAGCGAAGTGTCCACCCGTGC
  CAGGTCCAAGCTGCCGTCCGGCAAAAACATTCTGGTGTTCGGTGAAGATGGTTCTGGTAAGACAACTCTC
  ATGACCAAACTCCAAGGAGCAGAACACGGCAAAAAAGGAAGAGGCTTAGAGTATCTTTACCTCAGCGTTC
  ATGATGAGGACCGGGACGATCACACACGCTGCAATGTGTGGATTTTAGATGGAGACCTCTACCATAAAGG
  CCTGCTGAAATTTGCAGTTTCAGCTGAGTCTCTGCGGGAGACCCTGGTCATTTTTGTTGCCGACATGTCT
  CGACCTTGGACGATAATGGAGTCTCTGCAGAAGTGGGCTAGTGTTTTACGGGAGCACATTGACAAGATGA
  AGATTCCACCGGAGGAGATGCGGGATCTGGAACGGAAATTTATGAAAGAGTTTCAAGATTATATCGAGCC
  TGAAGAAGGTTGTCAGGGTTCCCCACAGAGAAGAGGGCCTCTGACCTCAGGCTCAGACGAAGACAGTGTT
  GCCCTCCCTCTGGGTGACAACGTGCTGACTCATAACCTGGGGATCCCAGTGTTGGTGGTGTGCACAAAGT
  GTGATGCCATGAGCGTCTTGGAGAAGGAGCACGATTACAGGGACGAGCACCTGGACTTCATCCAGGCGCA
  CCTGCGGAGATTCTGCCTCCAGTATGGAGCAGCCTTGATTTACACCTCAGTGAAGGAAGAGAAAAACCTT
  GACTTGCTGTATAAGTACATTGTTCATAAAACCTATGGCTTCCACTTTACCATACCCGCCTTAGTTGTGG
  AAAAGGACGCGGTTTTTATACCTGCAGGCTGGGACAATGAAAAGAAAATAGCTATTTTACATGAAAATTT
  TACAACCGTGAAGCCAGAAGACGCGTATGAAGATTTTATTGTAAAGCCGCCTGTGAGAAAGCTGGTCCAT
  GACAAAAAGTTGGCAGCGGAGGATGAGCAGGTGTTCCTAATGAAGCAACAGTCACTCCTTGCCAAGCAGC
  CTGCCACGCCCACAAGAACCTCCGAATCCCCTGCCAGAGGACCCTCTGGCTCTCCAAGGACGCAGGGTCG
  GGGTGGACCAGCTAGTGTACCAAGTGCCTCCCCCGGAACTTCAGTAAAGAAGCCAGATCCAAACATCAAA
  AATAATGCGGCAAGTGAAGGGGTATTGGCCAGCTTCTTCAATAGTCTGTTGAGTAAAAAAACAGGCTCTC
  CCGGAAGTCCTAGTGCTGGTGGAGTGCAGAGCACAGCCAAGAAGTCAGGACAAAAGACTGTGTTGTCAAA
  TGTTCAGGAAGAACTGGATAGAATGACTCGAAAGCCAGACTCCATGGTAACAAACTCTTCAACAGAAAAT
  GAAGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207876 representing NM_001013380
  Red=Cloning site Green=Tags(s)
  
  MAPVGVEKKLLLGPNGPAVAAAGDLTSEEEEGQSLWSSILSEVSTRARSKLPSGKNILVFGEDGSGKTTL
  MTKLQGAEHGKKGRGLEYLYLSVHDEDRDDHTRCNVWILDGDLYHKGLLKFAVSAESLRETLVIFVADMS
  RPWTIMESLQKWASVLREHIDKMKIPPEEMRDLERKFMKEFQDYIEPEEGCQGSPQRRGPLTSGSDEDSV
  ALPLGDNVLTHNLGIPVLVVCTKCDAMSVLEKEHDYRDEHLDFIQAHLRRFCLQYGAALIYTSVKEEKNL
  DLLYKYIVHKTYGFHFTIPALVVEKDAVFIPAGWDNEKKIAILHENFTTVKPEDAYEDFIVKPPVRKLVH
  DKKLAAEDEQVFLMKQQSLLAKQPATPTRTSESPARGPSGSPRTQGRGGPASVPSASPGTSVKKPDPNIK
  NNAASEGVLASFFNSLLSKKTGSPGSPSAGGVQSTAKKSGQKTVLSNVQEELDRMTRKPDSMVTNSSTEN
  EA
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013380
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013380.1, NP_001013398.1
• RefSeq Size: 3145 bp
• RefSeq ORF: 1479 bp
• Locus ID: 234663
• Cytogenetics: 8 53.04 cM