Dis3 (BC027357) Mouse Tagged ORF Clone

CAT#: MG208938

Dis3 (GFP-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae) (cDNA clone MGC:37123 IMAGE:4952415)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dis3
• Synonyms: 2810028N01Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208938 representing BC027357
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAGGTCCAAGACGTTCTTGAAGAAGACCCGCGCGGGCGGCGTGGTGAAGATCGTGCGCGAGCACT
  ACCTGCGGGATGACATCGGCTGCGGCGCGCCGGCTTGCTCGGCCTGCGGCGGGGCGCACGCGGGCCCGGC
  CCTGGAGCTGCAGCCCCGGGACCAGGCGAGCAGCCTCTGCCCGTGGCCGCACTACCTTCTGCCGGACACC
  AATGTGCTGCTGCACCAGATTGATGTCCTCGAACACCCGGCCATCAAAAATGTCATTGTGCTACAAACAG
  TGATGCAAGAAGTGAGAAACCGGAGCGCCCCCATCTACAAGCGAATCAGGGATGTGACCAATAACCAGGA
  AAAGCATTTCTATACCTTCACTAATGAGCACCATAAAGAAACCTACATCGAGCAAGAGCAGGGAGAGAAT
  GCCAATGACAGGAATGACAGAGCCATCCGAGTCGCAGCGAAGTGGTACAACGAGCACCTGAAGAGGGTGG
  CAGCAGACAGTCAGCTGCAAGTTATCCTGATAACCAATGACAGGAAGAACAAAGAGAAAGCTGTGCAAGA
  GGGGATACCAGCCTTCACGTGTGAAGAATACGTAAAGAGCCTGACTGCTAACCCTGAACTTATAGACCGT
  CTTGCTTACTTGTCCGATGAAATGAATGAAATAGAAAGTGGGAAAATAATATTTTCAGAGCATCTTCCCT
  TAAGCAAGCTCCAACAAGGCATAAAATCTGGTTCCTATCTTCAAGGAACATTCAGAGCTAGCAGGGAAAA
  TTATTTGGAGGCTACAGTATGGATTCATGGAGACAAAGAAGAGGAAAAAGAGATACTTATACAGGGAATT
  AAGCATCTAAACAGAGCTGTGCATGAAGACATTGTGGCCGTGGAGCTACTGCCCAGGAGCCAGTGGGTGG
  CACCGTCTTCCGTGGTTTTAGACGATGAAGGTCAAAATGAAGACGATGTGGAGAAAGATGAGGAGAGAGA
  ACTCCTGCTTAAGACTGCTGTAAGTGAAAAAATGTTACGGCCTACAGGTCGAGTTGTGGGGATAATAAAA
  AGGAACTGGAGACCGTATTGTGGCATGCTTTCCAAGTCTGATATTAAGGAGTCAAGAAGACATCTCTTTA
  CACCCGCTGATAAGAGAATTCCACGAATTCGGATAGAAACCAGACAGGCTTCTGCGTTAGAAGGACGGAG
  AATTATTGTCGCTATTGATGGTTGGCCTAGAAATTCTAGATATCCAAATGGACACTTTGTAAAGAATTTA
  GGCGATGTTGGAGAGAAGGAGACAGAAACGGAAGTGTTGCTGCTCGAGCACGATGTTCCTCATCAGCCCT
  TTTCCCAGGCTGTGCTTAGCTTCCTGCCCAGGATGCCCTGGAGCATTACTGAGGAGGACATGAAAAACCG
  AGAAGACCTGAGACATCTGTGTGTTTGCAGTGTGGACCCTCCAGGGTGCACTGACATAGATGACGCTCTG
  CATTGTAGAGAGCTCAGCAATGGAAACTTGGAGGTTGGTGTTCATATTGCGGATGTTAGCCATTTTATCA
  GGCCAGGAAATGCGTTGGATCAAGAATCTGCAAGAAGAGGAACAACTGTTTATCTTTGTGAAAAGAGGAT
  TGACATGGTTCCAGAGTTGCTCAGCTCCAACCTCTGTTCCTTAAGATCCAACGTTGACAGGCTTCTCTTA
  CGTACGCGGAAGCACAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208938 representing BC027357
  Red=Cloning site Green=Tags(s)
  
  MLRSKTFLKKTRAGGVVKIVREHYLRDDIGCGAPACSACGGAHAGPALELQPRDQASSLCPWPHYLLPDT
  NVLLHQIDVLEHPAIKNVIVLQTVMQEVRNRSAPIYKRIRDVTNNQEKHFYTFTNEHHKETYIEQEQGEN
  ANDRNDRAIRVAAKWYNEHLKRVAADSQLQVILITNDRKNKEKAVQEGIPAFTCEEYVKSLTANPELIDR
  LAYLSDEMNEIESGKIIFSEHLPLSKLQQGIKSGSYLQGTFRASRENYLEATVWIHGDKEEEKEILIQGI
  KHLNRAVHEDIVAVELLPRSQWVAPSSVVLDDEGQNEDDVEKDEERELLLKTAVSEKMLRPTGRVVGIIK
  RNWRPYCGMLSKSDIKESRRHLFTPADKRIPRIRIETRQASALEGRRIIVAIDGWPRNSRYPNGHFVKNL
  GDVGEKETETEVLLLEHDVPHQPFSQAVLSFLPRMPWSITEEDMKNREDLRHLCVCSVDPPGCTDIDDAL
  HCRELSNGNLEVGVHIADVSHFIRPGNALDQESARRGTTVYLCEKRIDMVPELLSSNLCSLRSNVDRLLL
  RTRKHR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC027357
• ORF Size: 1700 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC027357, AAH27357
• RefSeq Size: 2996
• RefSeq ORF: 1700
• Locus ID: 72662