Vps35 (BC005469) Mouse Tagged ORF Clone

CAT#: MG210707

Vps35 (GFP-tagged) - Mouse vacuolar protein sorting 35 (cDNA clone MGC:6633 IMAGE:3492842)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Vps35
• Synonyms: AI647796|Mem3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210707 representing BC005469
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTACAACACAGCAGTCACCCCAGGATGAGCAGGAAAAACTTTTGGATGAAGCCATCCAGGCTGTGA
  AGGTTCAGTCATTCCAGATGAAAAGATGCCTGGACAAAAACAAGCTGATGGATGCTCTGAAGCATGCCTC
  CAATATGCTTGGAGAGCTCCGGACCTCTATGCTGTCACCAAAGAGTTACTATGAACTTTATATGGCTATT
  TCTGATGAACTGCACTACTTGGAGGTCTACCTGACTGATGAATTTGCTAAAGGAAGAAAGGTGGCAGATC
  TCTATGAACTTGTACAGTACGCGGGAAACATTATTCCAAGGCTTTATCTCTTGATCACAGTTGGAGTTGT
  ATATGTCAAGTCATTTCCTCAATCCAGGAAAGATATTTTGAAAGATTTGGTAGAAATGTGCCGTGGTGTG
  CAGCATCCGCTAAGGGGTTTGTTTCTTCGAAATTACCTTCTTCAGTGTACTAGGAACATTTTACCTGATG
  AAGGAGAGCCAACAGATGAAGAAACAACTGGTGATATCAGTGATTCCATGGATTTTGTACTACTCAACTT
  TGCAGAAATGAATAAGCTCTGGGTGCGGATGCAGCATCAAGGACATAGTCGAGATAGAGAAAAAAGAGAA
  CGAGAGAGACAAGAACTGAGAATTTTAGTGGGAACAAATTTGGTGCGCCTTAGTCAGTTGGAAGGTGTAA
  ATGTGGAACGTTACAAACAGATTGTTCTAACAGGCATTCTGGAGCAGGTTGTGAATTGTAGGGATGCTCT
  GGCTCAGGAGTATCTCATGGAGTGCATCATTCAGGTTTTTCCTGATGAGTTTCATCTTCAGACTTTGAAT
  CCTTTTCTTAGAGCTTGTGCTGAATTACACCAAAATGTAAATGTGAAAAACATAATCATTGCTTTAATTG
  ACAGATTAGCTTTATTTGCTCATCGTGAAGATGGACCCGGAATTCCAGCTGAGATTAAACTTTTTGACAT
  ATTTTCACAACAGGTGGCTACAGTGATACAGTCCAGACAAGACATGCCATCAGAGGATGTTGTATCTTTA
  CAAGTCTCTCTCATTAATCTTGCTATGAAATGTTACCCTGATCGTGTGGACTACGTCGATAAAGTTCTAG
  AAACAACAGTGGAGATATTCAATAAGCTTAACCTTGAACATATTGCTACCAGTAGTGCAGTTTCAAAGGA
  GCTTACCAGACTTTTGAAGATACCTGTTGATACTTACAACAATATCTTAACAGTATTAAAGTTAAAGCAT
  TTCCACCCACTTTTTGAGTACTTTGACTACGAGTCCAGAAAGAGCATGAGCTGTTATGTGCTTAGTAATG
  TTCTGGATTATAACACAGAAATCGTCTCTCAGGACCAGGTAGATTCCATAATGAATTTGGTGTCCACATT
  GATTCAGGATCAGCCAGACCAACCTGTAGAAGACCCTGATCCAGAAGACTTCGCTGATGAACAGAGCCTT
  GTTGGCCGATTTATTCATCTGCTACGTTCTGATGATCCTGACCAGCAGTATTTGATTTTGAATACGGCAC
  GAAAACATTTTGGGGCTGGTGGAAATCAGCGGATTCGCTTCACACTGCCACCTTTGGTATTTGCAGCTTA
  CCAGTTGGCTTTTCGATACAAAGAGAATTCCCAAATGGATGACAAGTGGGAAAAGAAATGCCAGAAGATA
  TTTTCATTTGCTCACCAGACTATCAGTGCTTTGATTAAAGCTGAGCTGGCTGAATTACCACTGAGACTTT
  TTCTTCAAGGAGCATTAGCTGCTGGAGAAATTGGCTTTGAAAATCATGAAACAGTAGCATATGAATTTAT
  GTCCCAGGCATTTTCTCTATATGAAGATGAAATCAGTGATTCTAAAGCACAGCTGGCTGCTATCACCTTG
  ATCATCGGTACTTTTGAGAGGATGAAATGCTTCAGTGAAGAGAATCATGAACCCTTGAGAACTCAGTGTG
  CACTTGCTGCATCAAAACTTCTGAAAAAACCAGATCAAGGCCGAGCCGTGAGCACATGTGCACATCTCTT
  TTGGTCTGGCCGAAACACAGACAAAAATGGGGAAGAGCTTCATGGAGGTAAAAGGGTCATGGAGTGCCTA
  AAGAAGGCACTAAAAATAGCAAATCAGTGCATGGACCCCTCTCTACAAGTTCAGCTCTTTATAGAGATTC
  TGAACAGGTACATCTATTTCTATGAAAAAGAAAATGATGCGGTAACCATTCAAGTCTTGAACCAACTTAT
  TCAAAAGATTCGAGAAGATCTCCCAAATCTTGAGTCCAGTGAAGAAACAGAACAAATAAACAAGCATTTT
  CACAACACATTGGAGCACTTGCGCTCAAGACGGGAATCACCAGAGTCTGAGGGGCCAATCTATGAAGGTC
  TCATCCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210707 representing BC005469
  Red=Cloning site Green=Tags(s)
  
  MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAI
  SDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGV
  QHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRE
  RERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLN
  PFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSL
  QVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKH
  FHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSL
  VGRFIHLLRSDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSQMDDKWEKKCQKI
  FSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITL
  IIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECL
  KKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHF
  HNTLEHLRSRRESPESEGPIYEGLIL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC005469
• ORF Size: 2390 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC005469, AAH05469
• RefSeq Size: 3295
• RefSeq ORF: 2390
• Locus ID: 65114