Timeless (BC058641) Mouse Tagged ORF Clone

CAT#: MG211559

Timeless (GFP-tagged) - Mouse timeless homolog (Drosophila) (cDNA clone MGC:73470 IMAGE:6837105)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Timeless
• Synonyms: tim, Debt69
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211559 representing BC058641
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTGTACATGATGAACTGTGAACTTCTAGCCACGTGTAGCGCCCTTGGGTACTTGGAAGGAGGGA
  CTTACCACAAGGAGCCGGATTGCCTGGAGAGTGTGAAGGATTTGATCCGATACCTGAGGCACGAGGATGA
  GACCCGAGATGTGCGGCAGCAGCTGGGAGCTGCACAGATCCTGCAGAGCGACCTCCTGCCAATCCTCACG
  CAGCATCGCCAGGACAAGCCTCTCTTCGATGCCGTGATCAGGCTGATGGTAAATTTGACACAGCCAGCCT
  TGCTCTGTTTTGGCAGCGTGCCTAAGGACTCCAGTGTACGGCACCATTTTCTGCAGGTTCTAACGTACCT
  GCAAGCCTACAAAGAGGCCTTTGCCAGTGAGAAGGCATTTGGAGTCCTCAGCGAGACCTTGTATGAATTG
  CTACAGCTGGGCTGGGAGGATCGGCAAGAAGAAGACAACTTGCTGATCGAGCGGATCCTTCTGCTGGTCA
  GAAATATTCTCCATGTCCCGGCCAACCTTGAGCAGGAGAAGAGTATCGATGATGATGCCAGCATCCACGA
  CCGTCTCCTTTGGGCAATTCACCTCAGTGGCATGGACGACTTGCTCCTCTTCCTGTCCAGCTCATCCGCC
  GAGCAGCAGTGGAGCCTCCATGTGCTGGAGATCATCTCCCTCATGTTCCGAGACCAGACCCCTGAGCAGC
  TAGCGGGAGTAGGGCAGGGACGCTTGGCTCAGGAGCGAAGCACGGATGTGGCAGAATTGGAGGTGCTGCG
  CCAACGGGAGATGGCGGAGAAGAGAGCTCGGGCCCTCCAGCGAGGAAACAGGCACTCTCGATTTGGGGGC
  TCCTACATTGTCCAGGGGTTGAAATCTATTGGGGAGAAGGATGTCGTCTTTCACAAAGGCCTTCACAATC
  TCCAGAACTACAGCTCAGATCTGGGAAAGCAGCCCAGGAGGGTGCCCAAGCGTCGTCAGGCTGCCCAGGA
  GCTGTCTGTCCATCGCCGCTCTGTCCTGAATGTGAGACTCTTCCTCAGAGACTTCTGCTCTGAGTTCCTG
  GAGAACTGCTACAACCCGCTCATGGGCGCGGTCAAGGATCATCTGCTTCGGGAGAGAGCGCAGCAGCATG
  ACGAGACTTACTACATGTGGGCAATGGCTTTCTTCATGGCCTTCAACCGAGCTGCCACCTTCCGCCCCGG
  CCTTGTTTCTGAGACCCTCAGTATCCGTACCTTTCACTTTGTGGAGCAGAACCTCACCAACTACTACGAG
  ATGATGCTGACAGACCGCAAGGAGGCCGCCTCCTGGGCGCGCAGGATGCACCTGGCCCTGAAGGCCTACC
  AGGAGCTGCTGGCCACGGTGAACGAGATGGACATGTGCCCAGATGAGGCTGTTAGGGAGAGCAGTCGTAT
  CATCAAAAACAACATTTTCTATATGATGGAGTACCGAGAACTATTCCTGGCGCTCTTTCGAAAGTTTGAT
  GAGAGATACCATCCACGCTCATTCCTTCGAGACCTGGTGGAAACCACCCACCTCTTCCTCAAAATGTTGG
  AGCGCTTTTGCCGGAGCCGCGGGAACCTGATGGTGCAGAACAAAAGAAAAAAGAGGAAAAAGAAAAAGAA
  GGTTCAGGACCAGGGTGTTGCTTTCTCACAAAGCCCCGGGGAGCTGGAGGCCATGTGGCCAGCCCTGGCA
  GAGCAGCTGCTGCAGTGTGCCCAGGACCCTGAGCTCAGTGTGGACCCCGTCGTTCCCTTTGATGCGGCCT
  CAGAGGTGCCAGTGGAGGAGCAGCGGGTAGAAGCCATGGTGAGGATCCAAGACTGCCTTACGGCTGGCCA
  GGCCCCGCAAGCCCTGGCCCTCCTGCGGTCTGCCCGGGAAGTGTGGCCTGAAGGAAATGCGTTTGGCTCT
  CCAGTCATTTCCCCAGGGGAAGAAATGCAGTTGCTGAAACAAATCCTCTCCACGCCCCTTCCCCGGCAGC
  AGGAGCCAGAAGAAGGAGATGCAGAGGAGGAAGAGGAAGAGGAGGAGGAAGAGGAGTTACAGGTGGTCCA
  GGTGTCAGAGAAGGAGTTTAACTTTCTGGAATACCTGAAACGCTTCGCATCCTCAACCATCGTGCGGGCC
  TACGTGCTTCTCCTGCGGAGCTACAGGCAGAACAGTGCTCACACCAACCACTGCATCGCCAAGATGCTGC
  ACCGGCTGGCCCATGGCCTGGGGATGGAAGCCCTGCTTTTCCAGCTGTCCCTGTTCTGCCTCTTCAATCG
  GCTGCTTAGTGACCCAGCTGCTGCGGCCTACAAAGAGCTAGTGACTTTTGCCAAATACATCATTGGCAAG
  TTCTTTGCGTTGGCTGCCGTGAACCAGAAAGCGTTTGTGGAGCTGCTGTTCTGGAAGAACACCGCAGTGG
  TTCGGGAAATGACCCAGGGATATGGCTCCCTCGACAGTGGGTCTTCCAGCCACAGAGCTCCTCTGTGGAG
  CCCTGAGGAAGAGGCCCAGCTTCAGGAACTATACCTCGCCCACAAGGATGTGGAAGGTCAAGATGTAGTG
  GAAACCATATTGGCGCACCTGAAAGTCGTTCCTCGAACACGCAAGCAGGTCATCCACCACCTGGTCCGGA
  TGGGCCTGGCCGACAGCGTCAAGGAGTTCCAGAAGAGGAAAGGGACCCAGATTGTCTTGTGGACGGAGGA
  CCAGGAGCTGGAGTTACAGCGGCTCTTTGAGGAGTTCCGGGACTCTGATGATGTTCTTGGTCAAATCATG
  AAGAATATCACAGCCAAACGTTCACGGGCTCGAGTAGTGGACAAACTGTTGGCCCTGGGGTTGGTGTCTG
  AGCGGAGGCAGCTATACAAGAAACGGAGAAAGAAGCTGGCGCCTTCTTGCATGAATGGAGAAAAGTCCCC
  GAGAGACCCCTGGCAGGAAGATCCGGAAGAGGAGCCAGAGCCAGGAGTCCCCGGAGAGCAGGGTCCCAGT
  GAGGAGCACCGGACAGAAGCCCTGAGAGCCCTTCTGTCAGCCCGTAAGAGGAAAGCAGGCCTGGGGCCTA
  CAGAAGAGGAGGCCACTGGGGAGGAAGAATGGAACTCAGCGCCCAAGAAGCGGCAACTGCTGGACAGCGA
  CGAAGAGGAAGATGATGAGGGGAGGAGGCAAGCAGTGTCGGGAACGCCAAGAGTCCACAGGAAGAAACGG
  TTTCAGATTGAGGATGAGGATGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211559 representing BC058641
  Red=Cloning site Green=Tags(s)
  
  MDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILT
  QHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYEL
  LQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSA
  EQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGG
  SYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFL
  ENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYE
  MMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFD
  ERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALA
  EQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGS
  PVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRA
  YVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGK
  FFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVV
  ETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIM
  KNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMNGEKSPRDPWQEDPEEEPEPGVPGEQGPS
  EEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQAVSGTPRVHRKKR
  FQIEDEDD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC058641
• ORF Size: 3176 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC058641, AAH58641
• RefSeq Size: 4002
• RefSeq ORF: 3176
• Locus ID: 21853
• Gene Summary: The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]