Pde8b (NM_001170669) Mouse Tagged ORF Clone

CAT#: MG215051

Pde8b (GFP-tagged) - Mouse phosphodiesterase 8B (Pde8b) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pde8b
• Synonyms: B230331L10Rik; C030047E14Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215051 representing NM_001170669
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCGCCCCCAGCATCCATGTCTCGCAGAGCGGTGTGATCTACTGCCGGGACTCGGATGAGTCCA
  ACTCGCCCCGCCAGACCAGCAGCGTGTCGCAGGGCCCCACGGCGCCCCTGCACGGCCTCTTCGTGCAGAC
  CGACGCCGCCGACGCCATGCCCCCGAGTCGCGCGGCGGGACCCCCGGGCGCTGTCCGAGTCCGCAGATCC
  CGGGCTGAACTGGGCAGCGGCAGCAGCACGGGGTCCTCGGGCCCCGCCACGACCACCTGCCGGGGCCGGA
  GGCGCCACTGCTGCAGCAGCGCCGAGGCCGAGACCCAGACTAGCTACACCAGCGTCAAGCAGGTGTCTTC
  TGCAGAGGTGCGCATTGGGCCCATGAGACTGACACAGGACCCTATCCAGGTTCTGCTAATCTTCGCAAAG
  GAAGATAGCCAGAGTGATGGCTTCTGGTGGGCCTGTGACAGAGCTGGCTACAGATGCAACATCGCTCGCA
  CTCCAGAGTCGGCCCTCGAGTGCTTTCTGGACAAGCATCATGAAATTATTGTGATCGATCACAGGCAGAG
  CCGGAACTTTGATGCAGAAGCCGTGTGCAGGTCAATCCGGGCCACGAACCCCTCTGAGCACACGGTGATC
  CTGGCTGTCGTTTCACAAGCATCTGATGACCATGAAGAGGCCTCAGTCCTTCCTCTTCTTCATGCAGGCT
  TCAACCGGAGATTTATGGAGAACAGCAGCATAATTGCTTGCTATAATGAACTGATTCAAATAGAACACGG
  GGAAGTTCGCTCACAGTTCAAATTACGGGCCTGTAATTCAGTGTTTACAGCGTTAGACCACTGTCACGAA
  GCCATAGAAATAACAAGTGATGACCATGTGATTCAGATTCACCGGATTCATCGTGACTCCGGGGACAACT
  CTCAGACAGAGCCTCATTCGTTCAGACACAAGAGCAGGAGGAAAGAGTCCATTGATGTGAAATCAATATC
  TTCTCGGGGCAGTGATGCGCCAAGCCTACAGAATCGCCGCTACCCCTCCATGGCCAGGATCCACTCCATG
  ACCATTGAGGCTCCCATCACAAAGGTTATAAATATAATCAATGCAGCTCAGGAGAACAGCCCAGTTACAG
  TAGCAGAGGCCTTGGACAGAGTCCTAGAAATTTTACGGACCACAGAACTGTATTCCCCTCAGCTGGGAAC
  CAAAGATGAGGATCCCCACACCAGTGACCTTGTTGGGGGCCTGATGACTGATGGCTTGAGGAGACTGTCA
  GGGAACGAGTATGTGTTTACAAAGAATGTGCACCACAGTCACAGTCACCTCTCGATGCCCATCACCATCA
  ATGATGTCCCCCCAAGTATCGCCCAGCTCCTTGATAATGAGGAAAGCTGGGACTTCAACATCTTTGAATT
  GGAAGCCGTTACACATAAAAGGCCACTTGTGTACCTGGGCTTAAAGGTCTTCTCTCGGTTTGGAGTATGT
  GAATTTTTAAACTGTACTGAAACCACTCTTCGGGCCTGGCTGCAAGTGATTGAAGCCAACTACCACTCCT
  CCAACGCCTACCACAACTCCACCCATGCTGCCGATGTACTGCATGCCACGGCTTTCTTCCTTGGAAAGGA
  AAGAGTGAAGGGAAGCCTGGACCAGCTGGATGAGGTGGCTGCTCTGATTGCTGCCACAGTCCACGACGTG
  GATCACCCCGGAAGGACCAACTCTTTCCTGTGCAACGCCGGCAGCGAGCTCGCTGTGCTCTACAATGACA
  CTGCAGTCTTGGAGAGTCACCACACAGCCCTGGCCTTCCAGCTCACAGTCAAGGACACCAAGTGCAACAT
  TTTCAAGAATATTGACAGGAACCACTATCGAACGCTGCGCCAGGCTATTATCGACATGGTTTTGGCTACA
  GAGATGACAAAACACTTCGAGCACGTGAACAAATTCGTGAACAGCATCAACAAGCCACTGGCAGCTGAGA
  GTGAGGGCAGTGATTGTGAATGCAACCCTACCGGGAAGAACTTTCCTGAGAACCAAATCCTCATCAAACG
  CATGATGATTAAGTGTGCCGACGTGGCCAACCCGTGCCGGCCCTTGGACCTGTGCATTGAATGGGCTGGA
  AGGATCTCCGAGGAGTACTTTGCTCAGACTGATGAAGAGAAGAGGCAGGGGCTGCCTGTGGTGATGCCAG
  TGTTCGACCGCAATACCTGCAGCATCCCCAAGTCCCAGATCTCCTTCATCGACTACTTCATAACAGACAT
  GTTTGATGCTTGGGATGCCTTCGCTCACCTGCCTGCCCTGATGCAGCACCTGGCCGATAACTATAAACAC
  TGGAAGACACTGGATGACCTCAAGTGCAAAACTCTGAGACTTCCATCTGACAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215051 representing NM_001170669
  Red=Cloning site Green=Tags(s)
  
  MGCAPSIHVSQSGVIYCRDSDESNSPRQTSSVSQGPTAPLHGLFVQTDAADAMPPSRAAGPPGAVRVRRS
  RAELGSGSSTGSSGPATTTCRGRRRHCCSSAEAETQTSYTSVKQVSSAEVRIGPMRLTQDPIQVLLIFAK
  EDSQSDGFWWACDRAGYRCNIARTPESALECFLDKHHEIIVIDHRQSRNFDAEAVCRSIRATNPSEHTVI
  LAVVSQASDDHEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVRSQFKLRACNSVFTALDHCHE
  AIEITSDDHVIQIHRIHRDSGDNSQTEPHSFRHKSRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSM
  TIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLS
  GNEYVFTKNVHHSHSHLSMPITINDVPPSIAQLLDNEESWDFNIFELEAVTHKRPLVYLGLKVFSRFGVC
  EFLNCTETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDV
  DHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDTKCNIFKNIDRNHYRTLRQAIIDMVLAT
  EMTKHFEHVNKFVNSINKPLAAESEGSDCECNPTGKNFPENQILIKRMMIKCADVANPCRPLDLCIEWAG
  RISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPALMQHLADNYKH
  WKTLDDLKCKTLRLPSDS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170669
• ORF Size: 2364 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001170669.1, NP_001164140.1
• RefSeq Size: 4619
• RefSeq ORF: 2367
• Locus ID: 218461