Spam1 (NM_009241) Mouse Tagged ORF Clone

CAT#: MG219759

Spam1 (GFP-tagged) - Mouse sperm adhesion molecule 1 (Spam1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Spam1
• Synonyms: AV039194; Ph-20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219759 representing NM_009241
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGAGTTGAGATTTAAGCACCTCTTTTGGGGGAGCTTTGTTGAATCTGGGGGCACATTCCAAACAG
  TGTTAATCTTCCTTTTGATTCCATGCTCCTTGACTGTGGATTATAGGGCAGCACCAATTTTATCAAATAC
  AACTTTCCTTTGGATTTGGAATGTCCCAACTGAACGTTGTGTAGGAAATGTTAATGATCCAATAGATCTG
  AGCTTCTTCTCTTTAATTGGAAGCCCCCGGAAAACTGCCACAGGGCAACCTGTCACATTATTTTATGTTG
  ATCGACTTGGTTTGTATCCTCACATAGATGCAAACCAAGCAGAACATTATGGAGGAATACCTCAGAGGGG
  CGATTATCAAGCTCATTTGCGCAAAGCTAAGACTGACATAGAGCATTACATTCCAGACGACAAATTGGGC
  TTAGCTATCATTGACTGGGAAGAATGGAGGCCTACCTGGTTGAGAAACTGGAAACCTAAGGATAACTACA
  GGAATAAGTCTATTGAATTGGTCCAATCAACTAATCCAGGACTTAGTATCACAGAAGCCACCCAGAAAGC
  CATACAACAATTTGAAGAGGCAGGAAGGAAGTTTATGGAAGGAACTTTACACCTGGGGAAATTCCTTCGA
  CCAAACCAGCTATGGGGTTATTATCTATTTCCTGATTGTTATAACAATAAGTTTCAAGACCCTAAGTATG
  ATGGGCAGTGCCCTGCTGTGGAAAAGAAAAGAAATGATAATCTTAAATGGCTATGGAAAGCAAGCACCGG
  CCTTTACCCATCTGTCTATTTGAAGAAAGACTTGAAGTCCAATCGACAAGCTACCCTCTATGTCCGCTAC
  CGAGTTGTGGAAGCTATCAGAGTGTCCAAGGTTGGGAATGCATCGGATCCAGTCCCGATTTTTGTCTATA
  TCCGTCTTGTTTTTACTGATCGTACCTCTGAATACCTTCTAGAGGATGACCTTGTGAATACAATTGGTGA
  AATTGTTGCTCTGGGTACCTCTGGAATTATAATATGGGATGCTATGAGTTTAGCACAACGTGCGGCAGGT
  TGCCCAATCCTACATAAATACATGCAGACGACCCTGAATCCATACATAGTCAATGTTACCCTAGCAGCCA
  AAATGTGCAGCCAAACACTTTGTAATGAGAAAGGCATGTGTTCAAGAAGAAAAGAAAGTTCAGATGTATA
  TCTTCACTTGAACCCAAGTCATTTTGATATTATGTTAACGGAAACTGGAAAGTACGAAGTTCTTGGCAAC
  CCCAGGGTTGGAGACTTAGAATACTTTTCTGAACATTTTAAATGCAGCTGTTTTAGCAGAATGACATGTA
  AGGAGACATCTGATGTAAAAAATGTACAAGACGTGAATGTGTGCGTCGGTGACAATGTTTGTATAAAAGC
  CAAGGTAGAACCCAACCCAGCCTTCTACCTCCTACCTGGCAAAAGCCTTCTATTTATGACAACACTTGGT
  CATGTGCTGTACCATCTGCCACAAGATATTTTTGTTTTTCCACGGAAGACACTAGTCAGTACTCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219759 representing NM_009241
  Red=Cloning site Green=Tags(s)
  
  MGELRFKHLFWGSFVESGGTFQTVLIFLLIPCSLTVDYRAAPILSNTTFLWIWNVPTERCVGNVNDPIDL
  SFFSLIGSPRKTATGQPVTLFYVDRLGLYPHIDANQAEHYGGIPQRGDYQAHLRKAKTDIEHYIPDDKLG
  LAIIDWEEWRPTWLRNWKPKDNYRNKSIELVQSTNPGLSITEATQKAIQQFEEAGRKFMEGTLHLGKFLR
  PNQLWGYYLFPDCYNNKFQDPKYDGQCPAVEKKRNDNLKWLWKASTGLYPSVYLKKDLKSNRQATLYVRY
  RVVEAIRVSKVGNASDPVPIFVYIRLVFTDRTSEYLLEDDLVNTIGEIVALGTSGIIIWDAMSLAQRAAG
  CPILHKYMQTTLNPYIVNVTLAAKMCSQTLCNEKGMCSRRKESSDVYLHLNPSHFDIMLTETGKYEVLGN
  PRVGDLEYFSEHFKCSCFSRMTCKETSDVKNVQDVNVCVGDNVCIKAKVEPNPAFYLLPGKSLLFMTTLG
  HVLYHLPQDIFVFPRKTLVSTP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009241
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009241.2
• RefSeq Size: 2096
• RefSeq ORF: 1539
• Locus ID: 20690
• Gene Summary: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. [UniProtKB/Swiss-Prot Function]