Atp2b2 (NM_001036684) Mouse Tagged ORF Clone

CAT#: MG223661

Atp2b2 (GFP-tagged) - Mouse ATPase Ca++ transporting plasma membrane 2 (Atp2b2) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atp2b2
• Synonyms: D6Abb2e; dfw; Gena300; jog; PMCA2; Tmy; wms; wri
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223661 representing NM_001036684
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGACATGACCAACAGCGACTTTTACTCCAAAAACCAAAGAAATGAGTCGAGCCATGGGGGCGAGT
  TCGGGTGCACCATGGAGGAGCTGCGCTCCCTCATGGAGCTGCGGGGCACCGAGGCTGTGGTCAAGATCAA
  GGAGACGTATGGGGACACTGAAGCCATCTGCCGGCGCCTCAAAACCTCGCCTGTTGAAGGTTTACCAGGC
  ACTGCTCCAGACTTGGAAAAGAGGAAACAGATTTTTGGGCAAAACTTCATACCTCCAAAGAAACCAAAAA
  CCTTCCTGCAGCTGGTGTGGGAAGCGCTACAGGACGTGACACTTATCATCCTGGAGATCGCGGCCATCAT
  CTCCCTGGGACTGTCCTTCTACCACCCACCGGGAGAGAGCAATGAAGGATGTGCCACGGCCCAGGGTGGG
  GCAGAGGATGAAGGTGAAGCAGAAGCAGGCTGGATTGAGGGGGCTGCCATCCTGCTGTCAGTCATCTGTG
  TGGTCCTGGTCACAGCCTTCAATGACTGGAGTAAGGAGAAACAGTTCCGGGGCCTGCAGAGCCGAATTGA
  GCAGGAGCAGAAGTTCACTGTGGTCCGGGCCGGCCAGGTGGTTCAGATCCCTGTGGCCGAGATCGTGGTC
  GGGGACATTGCCCAGATCAAATATGGTGACCTTCTTCCCGCTGATGGCCTCTTCATCCAGGGAAATGACC
  TCAAAATTGATGAAAGCTCACTCACAGGGGAATCTGACCAGGTGCGCAAGTCTGTGGATAAGGACCCCAT
  GTTGCTGTCAGGAACCCATGTGATGGAGGGCTCAGGACGGATGGTGGTTACTGCTGTGGGTGTGAACTCT
  CAGACTGGCATCATATTTACCCTGCTTGGGGCTGGTGGTGAAGAGGAAGAGAAGAAAGACAAAAAAGCCA
  AGCAGCAGGATGGGGCAGCTGCCATGGAGATGCAGCCTCTGAAGAGTGCAGAGGGCGGCGATGCAGATGA
  CAAGAAGAAAGCCAACATGCACAAGAAAGAGAAGTCGGTGCTTCAGGGCAAGCTCACCAAACTGGCTGTG
  CAGATAGGCAAGGCGGGCCTGGTGATGTCGGCCATCACAGTGATCATCCTGGTACTCTACTTCACCGTGG
  ACACCTTCGTGGTCAACAAGAAGCCATGGCTGACGGAATGCACACCCGTCTACGTACAGTACTTTGTCAA
  GTTCTTCATCATTGGTGTGACGGTGCTGGTGGTCGCTGTGCCCGAGGGCCTCCCTCTGGCTGTCACCATC
  TCACTGGCCTATTCTGTGAAGAAAATGATGAAGGACAACAACCTGGTACGCCACCTGGATGCCTGTGAGA
  CCATGGGCAATGCCACAGCCATCTGCTCAGACAAGACAGGAACGCTGACCACCAACCGCATGACCGTGGT
  CCAGGCCTATGTCGGTGACGTCCACTACAAGGAGATCCCCGATCCCAGCTCCATCAATGCCAAGACGCTG
  GAGCTGCTGGTCAACGCCATTGCCATCAACAGCGCCTACACCACCAAGATCCTTCCCCCAGAAAAAGAGG
  GAGCCCTGCCCCGGCAGGTGGGCAACAAGACAGAGTGCGGCCTGCTGGGCTTTGTGCTGGACTTGAGGCA
  GGACTACGAGCCGGTGCGCAGCCAGATGCCAGAGGAGAAGCTGTATAAGGTGTACACCTTCAACTCCGTG
  CGCAAGTCCATGAGCACCGTCATCAAGATGCCCGACGAGAGCTTCCGCATGTACAGCAAGGGCGCCTCGG
  AGATTGTGCTCAAAAAGTGCTGCAAGATCCTCAGTGGGGCAGGGGAAGCCCGTGTCTTCCGGCCCCGAGA
  CAGGGATGAGATGGTTAAGAAGGTGATTGAGCCCATGGCCTGTGACGGGCTCCGTACCATCTGCGTGGCC
  TATCGTGACTTCCCCAGCAGCCCTGAGCCTGACTGGGACAATGAGAATGACATTCTCAATGAACTCACGT
  GCATCTGCGTGGTGGGCATCGAAGACCCAGTACGACCTGAGGTCCCAGAAGCCATCCGCAAGTGCCAGCG
  GGCAGGTATCACAGTCCGCATGGTCACCGGTGACAATATCAACACAGCCCGGGCCATCGCCATCAAGTGT
  GGCATTATCCACCCTGGAGAGGACTTCCTGTGCCTGGAAGGCAAAGAATTCAATCGGAGGATTCGCAACG
  AGAAGGGGGAGATTGAGCAGGAGCGGATTGACAAGATCTGGCCAAAGCTGAGGGTGCTGGCTCGCTCCTC
  GCCCACGGATAAGCACACGCTGGTCAAAGGCATCATCGACAGTACACACACTGAGCAGCGGCAGGTGGTG
  GCTGTGACAGGGGATGGGACCAACGACGGGCCTGCTCTCAAGAAGGCAGATGTGGGCTTCGCAATGGGCA
  TCGCAGGCACAGATGTGGCCAAGGAGGCCTCAGACATCATCCTGACAGATGACAACTTCAGCAGCATCGT
  CAAGGCAGTGATGTGGGGCCGTAACGTCTATGACAGCATATCCAAATTCCTGCAGTTCCAGCTGACTGTC
  AACGTGGTGGCGGTGATCGTGGCCTTCACGGGCGCCTGCATTACACAGGACTCCCCTCTCAAGGCTGTGC
  AGATGCTCTGGGTGAACCTCATCATGGACACGTTTGCCTCCCTGGCCCTGGCCACAGAGCCACCTACGGA
  GACTCTGCTTCTGAGGAAACCGTACGGTCGCAACAAGCCGCTCATCTCGAGGACCATGATGAAGAACATC
  CTGGGCCACGCCGTCTACCAGCTCACCCTCATCTTCACCCTGCTCTTCGTGGGTGAGAAGATGTTCCAGA
  TCGACAGCGGAAGGAACGCCCCGCTGCACTCACCACCCTCAGAGCACTACACCATCATCTTCAACACCTT
  CGTCATGATGCAGCTTTTCAACGAGATCAACGCCCGCAAGATCCACGGCGAGCGCAACGTCTTTGACGGG
  ATCTTCCGGAACCCCATCTTCTGCACCATCGTTCTTGGCACTTTCGCCATCCAGATAGTGATCGTGCAGT
  TTGGCGGGAAGCCCTTCAGCTGCTCCCCACTCCAGCTGGACCAGTGGATGTGGTGCATCTTCATAGGCCT
  GGGAGAGCTCGTCTGGGGCCAGGTCATCGCCACCATCCCTACCAGCAGGCTCAAGTTCCTGAAAGAGGCA
  GGGCGTCTAACACAGAAGGAGGAGATCCCCGAGGAGGAGTTAAATGAGGATGTGGAAGAGATAGACCACG
  CAGAGCGGGAGCTTCGCCGTGGCCAGATCCTGTGGTTCCGGGGCCTGAATCGGATCCAGACACAGATCCG
  CGTCGTGAAGGCGTTCCGTAGCTCTCTCTATGAAGGGTTAGAAAAACCCGAGTCTCGAACCTCCATCCAT
  AACTTCATGGCTCATCCTGAATTCCGGATCGAAGATTCCCAGCCCCACATCCCCCTCATCGATGACACCG
  ACCTGGAAGAAGATGCCGCGCTCAAGCAGAACTCGAGCCCGCCGTCCTCGCTCAACAAGAACAATAGCGC
  CATCGACAGCGGGATCAACCTGACGACCGACACGAGCAAATCAGCTACCTCTTCAAGTCCAGGGAGCCCC
  ATCCACAGCCTGGAGACGTCGCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223661 representing NM_001036684
  Red=Cloning site Green=Tags(s)
  
  MGDMTNSDFYSKNQRNESSHGGEFGCTMEELRSLMELRGTEAVVKIKETYGDTEAICRRLKTSPVEGLPG
  TAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYHPPGESNEGCATAQGG
  AEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVV
  GDIAQIKYGDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMVVTAVGVNS
  QTGIIFTLLGAGGEEEEKKDKKAKQQDGAAAMEMQPLKSAEGGDADDKKKANMHKKEKSVLQGKLTKLAV
  QIGKAGLVMSAITVIILVLYFTVDTFVVNKKPWLTECTPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTI
  SLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQAYVGDVHYKEIPDPSSINAKTL
  ELLVNAIAINSAYTTKILPPEKEGALPRQVGNKTECGLLGFVLDLRQDYEPVRSQMPEEKLYKVYTFNSV
  RKSMSTVIKMPDESFRMYSKGASEIVLKKCCKILSGAGEARVFRPRDRDEMVKKVIEPMACDGLRTICVA
  YRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKC
  GIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTHTEQRQVV
  AVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTV
  NVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTETLLLRKPYGRNKPLISRTMMKNI
  LGHAVYQLTLIFTLLFVGEKMFQIDSGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIHGERNVFDG
  IFRNPIFCTIVLGTFAIQIVIVQFGGKPFSCSPLQLDQWMWCIFIGLGELVWGQVIATIPTSRLKFLKEA
  GRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEGLEKPESRTSIH
  NFMAHPEFRIEDSQPHIPLIDDTDLEEDAALKQNSSPPSSLNKNNSAIDSGINLTTDTSKSATSSSPGSP
  IHSLETSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001036684
• ORF Size: 3594 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001036684.2, NP_001031761.1
• RefSeq Size: 6925 bp
• RefSeq ORF: 3597 bp
• Locus ID: 11941
• Cytogenetics: 6 52.85 cM